Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kamiya, H.; Iida, E.; Harashima, H.
    Important amino acids in the phosphohydrolase module of Escherichia coli Orf135 (2004), Biochem. Biophys. Res. Commun., 323, 1063-1068.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.65 E43A substrate 5-methyl-dCTP, about 20% of wild-type activity, substrate dCTP, 1%of wild-type activity Escherichia coli
3.6.1.65 E52A complete loss of activity Escherichia coli
3.6.1.65 E52D complete loss of activity Escherichia coli
3.6.1.65 E52Q complete loss of activity Escherichia coli
3.6.1.65 E55A substrate 5-methyl-dCTP, about 20% of wild-type activity, substrate dCTP, 1%of wild-type activity Escherichia coli
3.6.1.65 E55D substrate 5-methyl-dCTP, about 40% of wild-type activity, substrate dCTP, 2% of wild-type activity Escherichia coli
3.6.1.65 E55Q substrate 5-methyl-dCTP, about 110% of wild-type activity, substrate dCTP, 10% of wild-type activity Escherichia coli
3.6.1.65 E56A complete loss of activity Escherichia coli
3.6.1.65 E56D complete loss of activity Escherichia coli
3.6.1.65 E56Q complete loss of activity Escherichia coli
3.6.1.65 G36A complete loss of activity Escherichia coli
3.6.1.65 G37A complete loss of activity Escherichia coli
3.6.1.65 K38A substrate 5-methyl-dCTP, about 30% of wild-type activity, substrate dCTP, 10% of wild-type activity Escherichia coli
3.6.1.65 K38R substrate 5-methyl-dCTP, about 40% of wild-type activity, substrate dCTP, 5% of wild-type activity Escherichia coli
3.6.1.65 L53A substrate 5-methyl-dCTP, about 30% of wild-type activity, substrate dCTP, 30% of wild-type activity Escherichia coli
3.6.1.65 R51A substrate 5-methyl-dCTP, about 5% of wild-type activity, substrate dCTP, 1% of wild-type activity Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.65 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.65 2-hydroxy-dATP + H2O
-
 Escherichia coli 2-hydroxy-dAMP + diphosphate
-
 ?
3.6.1.65 5-methyl-dCTP + H2O
-
 Escherichia coli 5-methyl-dCMP + diphosphate
-
 ?
3.6.1.65 8-hydroxy-dGTP + H2O
-
 Escherichia coli 8-hydroxy-dGMP + diphosphate
-
 ?
3.6.1.65 CTP + H2O
-
 Escherichia coli CMP + diphosphate
-
 ?
3.6.1.65 dCTP + H2O
-
 Escherichia coli dCMP + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.65 Orf135
-
 Escherichia coli