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Literature summary extracted from

  • Wu, J.F.; Sun, C.W.; Jiang, C.Y.; Liu, Z.P.; Liu, S.J.
    A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1: purification, properties, genetic cloning and expression in Escherichia coli (2005), Arch. Microbiol., 183, 1-8.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.74 expressed in Escherichia coli BL21(DE3) cells Comamonas testosteroni
1.13.11.76 expression in Escherichia coli Comamonas testosteroni

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.74 Ca2+ 79.4% residual activity at 2 mM Comamonas testosteroni
1.13.11.74 Cd2+ abolishes enzyme activity completely at 2 mM Comamonas testosteroni
1.13.11.74 Co2+ abolishes enzyme activity completely at 2 mM Comamonas testosteroni
1.13.11.74 Cu2+ 2.9% residual activity at 2 mM Comamonas testosteroni
1.13.11.74 EDTA
-
 Comamonas testosteroni
1.13.11.74 Fe2+ 57.4% residual activity at 2 mM Comamonas testosteroni
1.13.11.74 Fe3+ 4.4% residual activity at 2 mM Comamonas testosteroni
1.13.11.74 H2O2 abolishes enzyme activity completely at 2 mM Comamonas testosteroni
1.13.11.74 K3Fe(CN)6 41.2% residual activity at 2 mM Comamonas testosteroni
1.13.11.74 Mg2+ 91.2% residual activity at 2 mM Comamonas testosteroni
1.13.11.74 Mn2+ 30.9% residual activity at 2 mM Comamonas testosteroni
1.13.11.74 Ni2+ abolishes enzyme activity completely at 2 mM Comamonas testosteroni
1.13.11.74 Zn2+ abolishes enzyme activity completely at 2 mM Comamonas testosteroni
1.13.11.76 Ca2+ 2 mM, 79% residual activity Comamonas testosteroni
1.13.11.76 Cd2+ 2 mM abolishes enzyme activity completely Comamonas testosteroni
1.13.11.76 Co2+ 2 mM abolishes enzyme activity completely Comamonas testosteroni
1.13.11.76 Cu2+ 2 mM, 2.9% residual activity Comamonas testosteroni
1.13.11.76 EDTA 2 mM, 50% residual activity Comamonas testosteroni
1.13.11.76 Fe2+ 2 mM, 57% residual activity. Not inhibitory in presence of ascorbate Comamonas testosteroni
1.13.11.76 H2O2 2 mM, complete loss of activity Comamonas testosteroni
1.13.11.76 Mg2+ 2 mM, 91% residual activity Comamonas testosteroni
1.13.11.76 Mn2+ 2 mM, 31% residual activity Comamonas testosteroni
1.13.11.76 Ni2+ 2 mM abolishes enzyme activity completely Comamonas testosteroni
1.13.11.76 Zn2+ 2 mM abolishes enzyme activity completely Comamonas testosteroni

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.74 0.00077
-
 2-amino-5-chlorophenol in 10 mM sodium phosphate buffer, at pH 8.0 and 22°C Comamonas testosteroni
1.13.11.74 0.00089
-
 2-Aminophenol in 10 mM sodium phosphate buffer, at pH 8.0 and 22°C Comamonas testosteroni
1.13.11.76 0.00077
-
 2-amino-5-chlorophenol pH 8.0, 22°C Comamonas testosteroni
1.13.11.76 0.00089
-
 2-Aminophenol pH 8.0, 22°C Comamonas testosteroni

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.74 33000
-
 2 * 38000 + 2 * 33000, SDS-PAGE Comamonas testosteroni
1.13.11.74 38000
-
 2 * 38000 + 2 * 33000, SDS-PAGE Comamonas testosteroni
1.13.11.74 130000
-
 gel filtration Comamonas testosteroni
1.13.11.76 33000
-
 2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE Comamonas testosteroni
1.13.11.76 38000
-
 2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE Comamonas testosteroni
1.13.11.76 130000
-
 gel filtration Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.74 2-amino-5-chlorophenol + O2 Comamonas testosteroni 68% activity compared to 2-aminophenol ?
-
 ?
1.13.11.74 2-amino-5-chlorophenol + O2 Comamonas testosteroni CNB-1 68% activity compared to 2-aminophenol ?
-
 ?
1.13.11.74 2-aminophenol + O2 Comamonas testosteroni 100% activity 2-aminomuconate 6-semialdehyde
-
 ?
1.13.11.74 2-aminophenol + O2 Comamonas testosteroni CNB-1 100% activity 2-aminomuconate 6-semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.74 Comamonas testosteroni Q6J1Z5 and Q6J1Z6 subunit alpha and catalytic subunit beta
-
1.13.11.74 Comamonas testosteroni CNB-1 Q6J1Z5 and Q6J1Z6 subunit alpha and catalytic subunit beta
-
1.13.11.76 Comamonas testosteroni Q6J1Z5 subunit alpha
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.74 ammonium sulfate precipitation, Mono Q column chromatography, and Superdex 200 gel filtration Comamonas testosteroni
1.13.11.76
-
 Comamonas testosteroni

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.76 29.7
-
 pH 8.0, 22°C Comamonas testosteroni

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.74 2-amino-5-chlorophenol + O2 68% activity compared to 2-aminophenol Comamonas testosteroni ?
-
 ?
1.13.11.74 2-amino-5-chlorophenol + O2 68% activity compared to 2-aminophenol Comamonas testosteroni CNB-1 ?
-
 ?
1.13.11.74 2-aminophenol + O2 100% activity Comamonas testosteroni 2-aminomuconate 6-semialdehyde
-
 ?
1.13.11.74 2-aminophenol + O2 100% activity Comamonas testosteroni CNB-1 2-aminomuconate 6-semialdehyde
-
 ?
1.13.11.74 additional information 5% activity with catechol Comamonas testosteroni ?
-
 ?
1.13.11.74 additional information 5% activity with catechol Comamonas testosteroni CNB-1 ?
-
 ?
1.13.11.74 protocatechuate + O2 33% activity compared to 2-aminophenol Comamonas testosteroni ?
-
 ?
1.13.11.74 protocatechuate + O2 33% activity compared to 2-aminophenol Comamonas testosteroni CNB-1 ?
-
 ?
1.13.11.76 2-amino-5-chlorophenol + O2 68% of the activity with 2-aminophenol Comamonas testosteroni 2-amino-5-chloromuconate 6-semialdehyde
-
 ?
1.13.11.76 2-aminophenol + O2
-
 Comamonas testosteroni ?
-
 ?
1.13.11.76 catechol + O2 5% of the activity with 2-aminophenol Comamonas testosteroni ?
-
 ?
1.13.11.76 additional information no substrates: 4-methylcatechol, 4-chlorocatechol, 2,4-dihydroxybenzoic acid, o-nitrophenol, p-nitrophenol, and 4-nitrocatechol Comamonas testosteroni ?
-
 ?
1.13.11.76 protocatechuate + O2 33% of the activity with 2-aminophenol Comamonas testosteroni ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.74 heterotetramer 2 * 38000 + 2 * 33000, SDS-PAGE Comamonas testosteroni
1.13.11.76 tetramer 2 * 33000, alpha-subunit, + 2 * 38000, beta-subunit, SDS-PAGE Comamonas testosteroni

Synonyms

EC Number Synonyms Comment Organism
1.13.11.76 CnbcA
-
 Comamonas testosteroni

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.13.11.74 2
-
 in 10 mM sodium phosphate buffer, at pH 8.0 and 22°C Comamonas testosteroni EDTA