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Literature summary extracted from

  • Chen, Z.G.; Fujii, I.; Ebizuka, Y.; Sankawa, U.
    Emodin O-methyltransferase from Aspergillus terreus (1992), Arch. Microbiol., 158, 29-34.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.283 4-chloromercuribenzene sulfonate potent inhibition Aspergillus terreus
2.1.1.283 iodoacetate slight inhibition Aspergillus terreus
2.1.1.283 additional information no inhibition by metal chelating agents Aspergillus terreus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.283 0.00034
-
 emodin pH 8.0, 30°C Aspergillus terreus
2.1.1.283 0.0041
-
 S-adenosyl-L-methionine pH 8.0, 30°C Aspergillus terreus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.283 additional information Mg2+ or Mn2+ is not an absolute requirement, nor increases the enzyme activity Aspergillus terreus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.283 53600
-
 6 * 53600, SDS-PAGE Aspergillus terreus
2.1.1.283 322000
-
 gel filtration Aspergillus terreus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.283 S-adenosyl-L-methionine + emodin Aspergillus terreus the enzyme is involved in biosynthesis of the seco-anthraquinone (+)-geodin S-adenosyl-L-homocysteine + questin
-
 ?
2.1.1.283 S-adenosyl-L-methionine + emodin Aspergillus terreus IMI 16043 the enzyme is involved in biosynthesis of the seco-anthraquinone (+)-geodin S-adenosyl-L-homocysteine + questin
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.283 Aspergillus terreus
-
-
-
2.1.1.283 Aspergillus terreus IMI 16043
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.283
-
 Aspergillus terreus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.283 mycelium
-
 Aspergillus terreus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.283 3.18
-
 pH 8.0, 30°C Aspergillus terreus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.283 S-adenosyl-L-methionine + 1,3,8-trihydroxy-6-methoxy-9,10-anthraquinone i.e. citreorosein, 22% of the activity compared to emodin Aspergillus terreus ?
-
 ?
2.1.1.283 S-adenosyl-L-methionine + 1,3,8-trihydroxy-6-methoxy-9,10-anthraquinone i.e. citreorosein, 22% of the activity compared to emodin Aspergillus terreus IMI 16043 ?
-
 ?
2.1.1.283 S-adenosyl-L-methionine + 7-chloroemodin 18% of the activity compared to emodin Aspergillus terreus ?
-
 ?
2.1.1.283 S-adenosyl-L-methionine + 7-chloroemodin 18% of the activity compared to emodin Aspergillus terreus IMI 16043 ?
-
 ?
2.1.1.283 S-adenosyl-L-methionine + catenarin 80% of the activity compared to emodin Aspergillus terreus ?
-
 ?
2.1.1.283 S-adenosyl-L-methionine + catenarin 80% of the activity compared to emodin Aspergillus terreus IMI 16043 ?
-
 ?
2.1.1.283 S-adenosyl-L-methionine + emodin
-
 Aspergillus terreus S-adenosyl-L-homocysteine + questin
-
 ?
2.1.1.283 S-adenosyl-L-methionine + emodin the enzyme is involved in biosynthesis of the seco-anthraquinone (+)-geodin Aspergillus terreus S-adenosyl-L-homocysteine + questin
-
 ?
2.1.1.283 S-adenosyl-L-methionine + emodin
-
 Aspergillus terreus IMI 16043 S-adenosyl-L-homocysteine + questin
-
 ?
2.1.1.283 S-adenosyl-L-methionine + emodin the enzyme is involved in biosynthesis of the seco-anthraquinone (+)-geodin Aspergillus terreus IMI 16043 S-adenosyl-L-homocysteine + questin
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.283 homohexamer 6 * 53600, SDS-PAGE Aspergillus terreus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.283 EOMT
-
 Aspergillus terreus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.283 30
-
 assay at Aspergillus terreus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.283 7 8
-
 Aspergillus terreus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.283 5.7 9 half maximal activity at pH 5.7 and pH 9.0 Aspergillus terreus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.283 Aspergillus terreus isoelectric focusing
-
 4.4