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Literature summary extracted from

  • Dommaraju, S.R.; Dogovski, C.; Czabotar, P.E.; Hor, L.; Smith, B.J.; Perugini, M.A.
    Catalytic mechanism and cofactor preference of dihydrodipicolinate reductase from methicillin-resistant Staphylococcus aureus (2011), Arch. Biochem. Biophys., 512, 167-174.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.17.1.8 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.8 dihydrodipicolinate the enzyme is inhibited by high concentrations of dihydrodipicolinate when using NADPH as a cofactor, but not with NADH Staphylococcus aureus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.8 0.012
-
 NADPH in HEPES buffer, pH 8.0, at 30°C Staphylococcus aureus
1.17.1.8 0.026
-
 NADH in HEPES buffer, pH 8.0, at 30°C Staphylococcus aureus
1.17.1.8 0.028
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate using NADPH as cosubstrate, in HEPES buffer, pH 8.0, at 30°C Staphylococcus aureus
1.17.1.8 0.039
-
 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate using NADH as cosubstrate, in HEPES buffer, pH 8.0, at 30°C Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.8 Staphylococcus aureus
-
-
-
1.17.1.8 Staphylococcus aureus MRSA
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.8 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+
-
 Staphylococcus aureus (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O
-
 ir
1.17.1.8 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADH + H+
-
 Staphylococcus aureus MRSA (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD+ + H2O
-
 ir
1.17.1.8 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+
-
 Staphylococcus aureus (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O
-
 ir
1.17.1.8 (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NADPH + H+
-
 Staphylococcus aureus MRSA (S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NADP+ + H2O
-
 ir

Synonyms

EC Number Synonyms Comment Organism
1.17.1.8 DHDPR
-
 Staphylococcus aureus
1.17.1.8 dihydrodipicolinate reductase
-
 Staphylococcus aureus
1.17.1.8 EC 1.3.1.26 formerly Staphylococcus aureus

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.8 NADH
-
 Staphylococcus aureus
1.17.1.8 NADPH NADPH is a more efficient cofactor than NADH Staphylococcus aureus