Literature summary extracted from
Kronberg, F.; Giacometti, R.; Ruiz-Herrera, J.; Passeron, S.
Characterization of the regulatory subunit of Yarrowia lipolytica cAMP-dependent protein kinase. Evidences of a monomeric protein (2011), Arch. Biochem. Biophys., 509, 66-75.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.11.11 |
gene RKA1, regulatory subunit, located in the F chromosome, expression of His6-tagged R subunit in Escherichia coli strain BL21(DE3) |
Yarrowia lipolytica |
2.7.11.11 |
gene TPK1 |
Yarrowia lipolytica |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.11.11 |
Mg2+ |
required |
Yarrowia lipolytica |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.7.11.11 |
42800 |
- |
1 * 42800, about, regulatory subunit, + 1 x 39000, catalytic subunit, sequence calculation |
Yarrowia lipolytica |
2.7.11.11 |
48000 |
- |
1 * 48000, isolated recombinant His-tagged regulatory subunit, SDS-PAGE |
Yarrowia lipolytica |
2.7.11.11 |
90000 |
- |
holoenzyme, gel filtration |
Yarrowia lipolytica |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.11.11 |
Yarrowia lipolytica |
C3W7W0 |
catalytic subunit, gene TPK1; strains PO1a and AC11, genes TPK1 and RKA1 encoding catalytic and regulatory subunits |
- |
2.7.11.11 |
Yarrowia lipolytica |
F2XG10 |
regulatory subunit, gene RKA1; strains PO1a and AC11, genes TPK1 and RKA1 encoding catalytic and regulatory subunits |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
2.7.11.11 |
phosphoprotein |
the catalytic subunits phosphorylate the regulatory subunits |
Yarrowia lipolytica |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.11.11 |
native catalytic subunit from by anion exchange and cAMP affinity chromatography |
Yarrowia lipolytica |
2.7.11.11 |
recombinant His6-tagged R subunit from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography |
Yarrowia lipolytica |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.11.11 |
dimer |
1 * 42800, about, regulatory subunit, + 1 x 39000, catalytic subunit, sequence calculation |
Yarrowia lipolytica |
2.7.11.11 |
monomer |
1 * 48000, isolated recombinant His-tagged regulatory subunit, SDS-PAGE |
Yarrowia lipolytica |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.7.11.11 |
PKA |
- |
Yarrowia lipolytica |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.7.11.11 |
30 |
- |
assay at |
Yarrowia lipolytica |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
2.7.11.11 |
7.5 |
- |
assay at |
Yarrowia lipolytica |
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
2.7.11.11 |
Yarrowia lipolytica |
regulatory subunit, sequence calculation |
- |
5.18 |