Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kronberg, F.; Giacometti, R.; Ruiz-Herrera, J.; Passeron, S.
    Characterization of the regulatory subunit of Yarrowia lipolytica cAMP-dependent protein kinase. Evidences of a monomeric protein (2011), Arch. Biochem. Biophys., 509, 66-75.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.11 gene RKA1, regulatory subunit, located in the F chromosome, expression of His6-tagged R subunit in Escherichia coli strain BL21(DE3) Yarrowia lipolytica
2.7.11.11 gene TPK1 Yarrowia lipolytica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.11 Mg2+ required Yarrowia lipolytica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.11 42800
-
1 * 42800, about, regulatory subunit, + 1 x 39000, catalytic subunit, sequence calculation Yarrowia lipolytica
2.7.11.11 48000
-
1 * 48000, isolated recombinant His-tagged regulatory subunit, SDS-PAGE Yarrowia lipolytica
2.7.11.11 90000
-
holoenzyme, gel filtration Yarrowia lipolytica

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.11 Yarrowia lipolytica C3W7W0 catalytic subunit, gene TPK1; strains PO1a and AC11, genes TPK1 and RKA1 encoding catalytic and regulatory subunits
-
2.7.11.11 Yarrowia lipolytica F2XG10 regulatory subunit, gene RKA1; strains PO1a and AC11, genes TPK1 and RKA1 encoding catalytic and regulatory subunits
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.11 phosphoprotein the catalytic subunits phosphorylate the regulatory subunits Yarrowia lipolytica

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.11 native catalytic subunit from by anion exchange and cAMP affinity chromatography Yarrowia lipolytica
2.7.11.11 recombinant His6-tagged R subunit from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography Yarrowia lipolytica

Subunits

EC Number Subunits Comment Organism
2.7.11.11 dimer 1 * 42800, about, regulatory subunit, + 1 x 39000, catalytic subunit, sequence calculation Yarrowia lipolytica
2.7.11.11 monomer 1 * 48000, isolated recombinant His-tagged regulatory subunit, SDS-PAGE Yarrowia lipolytica

Synonyms

EC Number Synonyms Comment Organism
2.7.11.11 PKA
-
Yarrowia lipolytica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.11 30
-
assay at Yarrowia lipolytica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.11 7.5
-
assay at Yarrowia lipolytica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.11.11 Yarrowia lipolytica regulatory subunit, sequence calculation
-
5.18