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Literature summary extracted from

  • Negre, F.; Kolosova, N.; Knoll, J.; Kish, C.M.; Dudareva, N.
    Novel S-adenosyl-L-methionine:salicylic acid carboxyl methyltransferase, an enzyme responsible for biosynthesis of methyl salicylate and methyl benzoate, is not involved in floral scent production in snapdragon flowers (2002), Arch. Biochem. Biophys., 406, 261-270.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.273 subcloned into expression vector pET-28a, containing an N-terminal polyhistidine 6-His-tag, and expressed in Escherichia coli Antirrhinum majus
2.1.1.274 subcloned into expression vector pET-28a, containing an N-terminal polyhistidine 6-His-tag, and expressed in Escherichia coli Antirrhinum majus

General Stability

EC Number General Stability Organism
2.1.1.273 the enzyme is active in both Tris- and phosphate–citrate-based buffers, although its activity in phosphate-citrate buffer is about 20% lower than in Tris-buffer Antirrhinum majus
2.1.1.274 the enzyme is active in both Tris- and phosphate–citrate-based buffers, although its activity in phosphate-citrate buffer is about 20% lower than in Tris-buffer Antirrhinum majus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.273 Cu2+ 5 mM, strong inhibitory effect with more than 95% inhibition Antirrhinum majus
2.1.1.273 Fe2+ 5 mM, strong inhibitory effect with more than 95% inhibition Antirrhinum majus
2.1.1.273 Mn2+ 5 mM, inhibits activity less than 30% Antirrhinum majus
2.1.1.273 additional information presence of 5 mM Mg2+ in the assay reaction and addition of K+, NH4+ and Ca2+ does not affect activity Antirrhinum majus
2.1.1.273 Na+ 5 mM, inhibits activity less than 30% Antirrhinum majus
2.1.1.273 Zn2+ 5 mM, inhibits activity less than 30% Antirrhinum majus
2.1.1.274 Cu2+ 5 mM, strong inhibitory effect with more than 95% inhibition Antirrhinum majus
2.1.1.274 Fe2+ 5 mM, strong inhibitory effect with more than 95% inhibition Antirrhinum majus
2.1.1.274 Mn2+ 5 mM, inhibits SAMT activity less than 30% Antirrhinum majus
2.1.1.274 additional information presence of 5 mM Mg2+ in the assay reaction and addition of K+, NH4+ and Ca2+ does not affect SAMT activity Antirrhinum majus
2.1.1.274 Na+ 5 mM, inhibits SAMT activity less than 30% Antirrhinum majus
2.1.1.274 Zn2+ 5 mM, inhibits SAMT activity less than 30% Antirrhinum majus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.273 0.003
-
 S-adenosyl-L-methionine with benzoic acid, pH 7.5, 20°C Antirrhinum majus
2.1.1.273 1.72
-
 benzoic acid pH 7.5, 20°C Antirrhinum majus
2.1.1.274 0.004
-
 S-adenosyl-L-methionine with salicylic acid, pH 7.5, 20°C Antirrhinum majus
2.1.1.274 0.083
-
 salicylic acid pH 7.5, 20°C Antirrhinum majus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.273 49000
-
 SDS-PAGE Antirrhinum majus
2.1.1.273 107000
-
 with a calibrated Sephacryl 200-HR column Antirrhinum majus
2.1.1.274 49000
-
 SDS-PAGE Antirrhinum majus
2.1.1.274 107000
-
 with a calibrated Sephacryl 200-HR column Antirrhinum majus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.273 Antirrhinum majus Q8H6N2 snapdragon
-
2.1.1.274 Antirrhinum majus Q8H6N2 snapdragon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.273 by nickel-based affinity chromatography Antirrhinum majus
2.1.1.274 by nickel-based affinity chromatography Antirrhinum majus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.273 additional information transcripts are undetectable in floral and leaf tissues by RNA-blot hybridization but low levels of gene expression detected with real-time RT-PCR Antirrhinum majus
-
2.1.1.273 petal 200fold higher levels of mRNA than in the pistil where lowest level of mRNA expression is observed Antirrhinum majus
-
2.1.1.273 sepal expression of mRNA about half the level in petal Antirrhinum majus
-
2.1.1.273 stamen expression of only 2fold higher than in pistil, where lowest level of mRNA expression is observed Antirrhinum majus
-
2.1.1.274 petal 200fold higher levels of SAMT mRNA than in the pistil, where lowest level of mRNA expression is observed Antirrhinum majus
-
2.1.1.274 sepal Expression of SAMT mRNA about half the level in petal, where lowest level of mRNA expression is observed Antirrhinum majus
-
2.1.1.274 stamen expression of SAMT only 2fold higher than in pistil, where lowest level of mRNA expression is observed Antirrhinum majus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.273 0.000054
-
 benzoic acid, pH 7.5, 20°C Antirrhinum majus
2.1.1.274 0.000126
-
 salicylic acid, pH 7.5, 20°C Antirrhinum majus

Storage Stability

EC Number Storage Stability Organism
2.1.1.273 the recombinant protein is catalytically stable for more than a year when stored at -80°C Antirrhinum majus
2.1.1.274 the recombinant protein is catalytically stable for more than a year when stored at -80°C Antirrhinum majus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.273 additional information enzyme is also active with salicylic acid resulting in methyl salicylate formation Antirrhinum majus ?
-
 ?
2.1.1.273 additional information no activity with 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, trans-cinnamic acid, p-coumaric acid, m-coumaric acid, o-coumaric acid and benzyl alcohol Antirrhinum majus ?
-
 ?
2.1.1.273 S-adenosyl-L-methionine + benzoic acid
-
 Antirrhinum majus S-adenosyl-L-homocysteine + methyl benzoate
-
 ?
2.1.1.274 additional information enzyme is also active with benzoic acid resulting in methyl benzoate formation Antirrhinum majus ?
-
 ?
2.1.1.274 additional information no activity with 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, trans-cinnamic acid, p-coumaric acid, m-coumaric acid, o-coumaric acid and benzyl alcohol Antirrhinum majus ?
-
 ?
2.1.1.274 S-adenosyl-L-methionine + salicylic acid
-
 Antirrhinum majus S-adenosyl-L-homocysteine + methyl salicylate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.273 homodimer 2 * 49000, SDS-PAGE Antirrhinum majus
2.1.1.274 homodimer 2 * 49000, SDS-PAGE Antirrhinum majus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.273 BAMT
-
 Antirrhinum majus
2.1.1.273 benzoic acid carboxyl methyltransferase
-
 Antirrhinum majus
2.1.1.274 salicylic acid carboxyl methyltransferase
-
 Antirrhinum majus
2.1.1.274 SAMT
-
 Antirrhinum majus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.1.273 additional information
-
 enzyme is stable at temperatures up to 42°C for 30 min but 30 min incubation at 65°C leads to a 70% loss of activity Antirrhinum majus
2.1.1.274 42
-
 enzyme is stable at temperatures up to 42°C for 30 min but 30-min incubation at 65°C leads to a 70% loss of activity Antirrhinum majus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.273 0.0058
-
 benzoic acid pH 7.5, 20°C Antirrhinum majus
2.1.1.273 0.006
-
 S-adenosyl-L-methionine with benzoic acid, pH 7.5, 20°C Antirrhinum majus
2.1.1.274 0.011
-
 salicylic acid pH 7.5, 20°C Antirrhinum majus
2.1.1.274 0.013
-
 S-adenosyl-L-methionine with salicylic acid, pH 7.5, 20°C Antirrhinum majus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.273 7.5
-
-
 Antirrhinum majus
2.1.1.274 7.5
-
-
 Antirrhinum majus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.273 additional information
-
 at pH 4.0 and 9.5 the enzyme activity falls to about 20% of the maximal activity Antirrhinum majus
2.1.1.273 5 8 with 80–90% of maximum activity Antirrhinum majus
2.1.1.274 additional information
-
 at pH 4.0 and 9.5 the enzyme activity falls to about 20% of the maximal activity Antirrhinum majus
2.1.1.274 5 8 with 80–90% of maximum activity Antirrhinum majus