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Literature summary extracted from

  • Nihira, T.; Nakai, H.; Chiku, K.; Kitaoka, M.
    Discovery of nigerose phosphorylase from Clostridium phytofermentans (2012), Appl. Microbiol. Biotechnol., 93, 1513-1522.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.279 expression in Escherichia coli Lachnoclostridium phytofermentans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.279 3-O-alpha-D-glucopyranosyl-D-glucopyranose
-
 Lachnoclostridium phytofermentans
2.4.1.279 Co2+ 1 mM, 80% inhibition Lachnoclostridium phytofermentans
2.4.1.279 Cu2+ 1 mM, 98.7% inhibition Lachnoclostridium phytofermentans
2.4.1.279 Fe2+ 1 mM, 68% inhibition Lachnoclostridium phytofermentans
2.4.1.279 additional information no inhibition by EDTA Lachnoclostridium phytofermentans
2.4.1.279 Ni2+ 1 mM, 95.4% inhibition Lachnoclostridium phytofermentans
2.4.1.279 Zn2+ 1 mM, 85% inhibition Lachnoclostridium phytofermentans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.279 0.2
-
 phosphate pH 7.0, 30°C, phosphorolysis reaction, cosubstrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose Lachnoclostridium phytofermentans
2.4.1.279 1.7
-
 3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C, phosphorolyis reaction Lachnoclostridium phytofermentans
2.4.1.279 3.3
-
 D-glucose pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 79
-
 1,5-anhydro-D-glucitol pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 86
-
 D-xylose pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 104
-
 D-galactose pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.279 cytosol
-
 Lachnoclostridium phytofermentans 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.279 additional information no divalent metal ions required Lachnoclostridium phytofermentans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.279 87170
-
 calculated from amino acid sequence Lachnoclostridium phytofermentans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.279 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway. D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.279 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Lachnoclostridium phytofermentans ATCC 700394 Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway. D-glucose + beta-D-glucose 1-phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.279 Lachnoclostridium phytofermentans A9KT32
-
-
2.4.1.279 Lachnoclostridium phytofermentans ATCC 700394 A9KT32
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.279 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate = D-glucose + beta-D-glucose 1-phosphate sequential bi-bi mechanism Lachnoclostridium phytofermentans
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.279 0.17
-
 pH 7.0, 30°C, phosphorolysis reaction, substrate 2-O-alpha-D-glucopyranosyl-D-glucopyranose Lachnoclostridium phytofermentans
2.4.1.279 0.17
-
 pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + methyl alpha-D-glucoside Lachnoclostridium phytofermentans
2.4.1.279 4.6
-
 pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-galactose Lachnoclostridium phytofermentans
2.4.1.279 8.1
-
 pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol Lachnoclostridium phytofermentans
2.4.1.279 9.3
-
 pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-xylose Lachnoclostridium phytofermentans
2.4.1.279 32
-
 pH 7.0, 30°C, phosphorolysis reaction, substrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose Lachnoclostridium phytofermentans
2.4.1.279 54
-
 pH 7.0, 30°C, reverse phosphorolysis reaction, substrates beta-D-glucose 1-phosphate + D-glucose Lachnoclostridium phytofermentans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.279 2-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate very weak phosphorolysis reaction with kojibiose, 0.5% of the activity with nigerose Lachnoclostridium phytofermentans D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.279 2-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate very weak phosphorolysis reaction with kojibiose, 0.5% of the activity with nigerose Lachnoclostridium phytofermentans ATCC 700394 D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.279 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway. Lachnoclostridium phytofermentans D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.279 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate phosphorolysis reaction with nigerose Lachnoclostridium phytofermentans D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.279 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate Nigerose may be liberated from nigeran outside the cell, transported into the cell by a sugar transporter and phosphorylyzed into beta-D-glucose 1-phosphate. This is converted into D-glucose 6-phosphate and can enter the glycolytic pathway. Lachnoclostridium phytofermentans ATCC 700394 D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.279 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate phosphorolysis reaction with nigerose Lachnoclostridium phytofermentans ATCC 700394 D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.279 beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol reverse phosphorolysis reaction 15% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate
-
 ?
2.4.1.279 beta-D-glucose 1-phosphate + 1,5-anhydro-D-glucitol reverse phosphorolysis reaction 15% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose Lachnoclostridium phytofermentans ATCC 700394 3-O-alpha-D-glucopyranosyl-1,5-anhydro-D-glucitol + phosphate
-
 ?
2.4.1.279 beta-D-glucose 1-phosphate + D-galactose reverse phosphorolysis reaction 8.5% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose, no significant preference for specific anomer of the acceptor Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-D-galactopyranose + phosphate
-
 ?
2.4.1.279 beta-D-glucose 1-phosphate + D-glucose reverse phosphorolysis reaction, no significant preference for specific anomer of the acceptor Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-D-glucopyranose + phosphate
-
 r
2.4.1.279 beta-D-glucose 1-phosphate + D-xylose reverse phosphorolysis reaction 17% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose Lachnoclostridium phytofermentans 3-O-alpha-D-glucopyranosyl-D-xylopyranose + 2-O-alpha-D-glucopyranosyl-D-xylopyranose + phosphate products are formed in the ratio 63:37% ?
2.4.1.279 beta-D-glucose 1-phosphate + methyl alpha-D-glucoside reverse phosphorolysis reaction 17% of the activity with 3-O-alpha-D-glucopyranosyl-D-glucopyranose Lachnoclostridium phytofermentans methyl-3-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside + methyl-2-O-alpha-D-glucopyranosyl-alpha-D-glucopyranoside + phosphate products are formed in the ratio 71:29% ?
2.4.1.279 additional information in the reverse phosphorolyis reaction the enzyme produces only disaccharides. The enzyme does not use any disaccharides as acceptors in the reverse phosphorolysis reaction. It does not use any D-glucose derivatives at the C2 position such as D-mannose, 2-deoxy-D-glucose, D-glucosamine, N-acetyl-D-glucosamine Lachnoclostridium phytofermentans ?
-
 ?
2.4.1.279 additional information in the reverse phosphorolyis reaction the enzyme produces only disaccharides. The enzyme does not use any disaccharides as acceptors in the reverse phosphorolysis reaction. It does not use any D-glucose derivatives at the C2 position such as D-mannose, 2-deoxy-D-glucose, D-glucosamine, N-acetyl-D-glucosamine Lachnoclostridium phytofermentans ATCC 700394 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.279 cphy1874
-
 Lachnoclostridium phytofermentans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.279 40
-
-
 Lachnoclostridium phytofermentans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.279 67
-
 phosphate pH 7.0, 30°C, cosubstrate 3-O-alpha-D-glucopyranosyl-D-glucopyranose Lachnoclostridium phytofermentans
2.4.1.279 69
-
 D-galactose pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 110
-
 D-glucose pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 118
-
 D-xylose pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 120
-
 1,5-anhydro-D-glucitol pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.279 7
-
-
 Lachnoclostridium phytofermentans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.279 5.5 9
-
 Lachnoclostridium phytofermentans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.279 6.4
-
 3-O-alpha-D-glucopyranosyl-D-glucopyranose pH 7.0, 30°C Lachnoclostridium phytofermentans

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.4.1.279 0.7
-
 D-galactose pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 1.4
-
 D-xylose pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 1.5
-
 1,5-anhydro-D-glucitol pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans
2.4.1.279 33
-
 D-glucose pH 7.0, 30°C, reverse phosphorolysis reaction, cosubstrate beta-D-glucose 1-phosphate Lachnoclostridium phytofermentans