EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.250 | gene tm0297, located in an operon with tm0298 encoding a mannitol dehydrogenase, phylogenetic analysis, expression of the Strep-tagged enzyme in Escherichia coli | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.250 | 0.027 | - |
NADH | with D-ribulose, pH 7.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
1.1.1.250 | 0.031 | - |
NADH | with D-ribulose, pH 7.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
1.1.1.250 | 0.059 | - |
NAD+ | with D-arabinitol, pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
1.1.1.250 | 0.069 | - |
NAD+ | with D-arabinitol, pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
1.1.1.250 | 2.6 | - |
D-arabinitol | pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
1.1.1.250 | 7.1 | - |
D-arabinitol | pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
1.1.1.250 | 7.2 | - |
D-ribulose | pH 7.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
1.1.1.250 | 13.9 | - |
D-ribulose | pH 7.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
1.1.1.250 | 20 | - |
D-xylitol | pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
1.1.1.250 | 21.7 | - |
D-xylitol | pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.250 | K+ | twofold activation at 60 mM, contributes to the overall stability of the protein and ise not required for catalytic activity | Thermotoga maritima | |
1.1.1.250 | Mg2+ | twofold activation at 55 mM, contributes to the overall stability of the protein and is not required for catalytic activity | Thermotoga maritima | |
1.1.1.250 | additional information | no effect on the activity by CaCl2 or NaCl | Thermotoga maritima |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.250 | 27600 | - |
6 * 27600, SDS-PAGE | Thermotoga maritima |
1.1.1.250 | 164000 | - |
native PAGE | Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.250 | Thermotoga maritima | - |
gene tm0297 | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.250 | additional information | Thermotoga maritima cannot use D-arabitol as a sole carbon source. Optimal growth temperature of Thermotoga maritima is 80°C | Thermotoga maritima | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.250 | D-arabinitol + NAD+ | - |
Thermotoga maritima | D-ribulose + NADH + H+ | - |
r | |
1.1.1.250 | D-xylitol + NAD+ | - |
Thermotoga maritima | D-xylulose + NADH + H+ | - |
r | |
1.1.1.250 | additional information | the enzyme exclusively catalyzed the NAD(H)-dependent oxidoreduction of D-arabitol, D-xylitol, D-ribulose, or D-xylulose | Thermotoga maritima | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.250 | homohexamer | 6 * 27600, SDS-PAGE | Thermotoga maritima |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.250 | arabitol 2-dehydrogenase | - |
Thermotoga maritima |
1.1.1.250 | ArDH | - |
Thermotoga maritima |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.250 | 85 | - |
- |
Thermotoga maritima |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.250 | 50 | 85 | inactive below 50°C, optimal activity at 85°C | Thermotoga maritima |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.250 | additional information | - |
the addition of the Strep-tag led to a destabilization of tmArDH resulting in the loss of thermostability | Thermotoga maritima |
1.1.1.250 | 85 | - |
rapid inactivation of the recombinant Strep-tagged enzyme, while the detagged enzyme retains over 90% activity after 90 min at 85°C | Thermotoga maritima |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.250 | 11 | - |
D-arabinitol | pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
1.1.1.250 | 11.2 | - |
D-xylitol | pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
1.1.1.250 | 16.6 | - |
D-xylitol | pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
1.1.1.250 | 18.7 | - |
D-arabinitol | pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
1.1.1.250 | 165.6 | - |
D-ribulose | pH 7.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
1.1.1.250 | 193.2 | - |
D-ribulose | pH 7.5, 85°C, recombinant detagged enzyme | Thermotoga maritima |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.250 | 7.5 | - |
reduction reaction | Thermotoga maritima |
1.1.1.250 | 8.5 | - |
oxidation reaction | Thermotoga maritima |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.250 | 4.5 | 9.5 | 40% and 10% of maximal activity at pH 5.5 and pH 8.5, no activity below pH 4.5 and above pH 9.5 | Thermotoga maritima |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.250 | NAD+ | dependent on | Thermotoga maritima | |
1.1.1.250 | NADH | dependent on | Thermotoga maritima |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.250 | 0.56 | - |
D-xylitol | pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
1.1.1.250 | 0.74 | - |
D-xylitol | pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
1.1.1.250 | 1.5 | - |
D-arabinitol | pH 8.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
1.1.1.250 | 7.2 | - |
D-arabinitol | pH 8.5, 85°C, recombinant detagged enzyme | Thermotoga maritima | |
1.1.1.250 | 12 | - |
D-ribulose | pH 7.5, 85°C, recombinant Strep-tagged enzyme | Thermotoga maritima | |
1.1.1.250 | 27 | - |
D-ribulose | pH 7.5, 85°C, recombinant detagged enzyme | Thermotoga maritima |