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Literature summary extracted from

  • Lindner, S.N.; Niederholtmeyer, H.; Schmitz, K.; Schoberth, S.M.; Wendisch, V.F.
    Polyphosphate/ATP-dependent NAD kinase of Corynebacterium glutamicum: biochemical properties and impact of ppnK overexpression on lysine production (2010), Appl. Microbiol. Biotechnol., 87, 583-593.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.23 expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.23 Ca2+ ATP-dependent activity is reduced to 72% in the presence of 20 mM Ca2+ Corynebacterium glutamicum
2.7.1.23 KCl 200 mM KCl decreases the polyphosphate-dependent activity to 75% Corynebacterium glutamicum
2.7.1.23 NaCl 200 mM NaCl decreases the polyphosphate-dependent activity to 75% Corynebacterium glutamicum
2.7.1.23 NADH
-
 Corynebacterium glutamicum
2.7.1.23 NADP+
-
 Corynebacterium glutamicum
2.7.1.23 Ni2+ ATP-dependent activity is reduced to 60% or 7% in the presence of 5 or 20 mM Ni2+ Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.23 0.49
-
 ATP in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 0.67
-
 NAD+ in the presence of ATP, in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 1.1
-
 NAD+ in the presence of polyphosphate(20), in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 2.1
-
 Tetraphosphate in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 2.4
-
 Triphosphate in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 3.1
-
 polyphosphate(75) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 4.3
-
 ADP in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 5.9
-
 polyphosphate(20) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 14.1
-
 polyphosphate(45) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 17.2
-
 polyphosphate(25) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 18.4
-
 polyphosphate(65) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.23 Mg2+ enzyme activity is enhanced by Mg2+. The highest activity for ATP is reached with 20 mM Mg2+ (2.6fold higher than with ATP, but without addition of bivalent cations) Corynebacterium glutamicum
2.7.1.23 Mn2+ enzyme activity is enhanced by Mn2+. When using polyphosphate, the enzyme requires bivalent cations, preferably manganese ions, for activity. With polyphosphate, the enzyme is most active in the presence of 15 mM Mn2+ (60% activity compared to ATP with 20 mM Mg2+). 20 mM Mn2+ increases ATP-dependent activity 1.9fold Corynebacterium glutamicum
2.7.1.23 additional information addition of NaCl or KCl does not alter ATP-dependent activity Corynebacterium glutamicum
2.7.1.23 additional information enzyme activity is independent of bivalent cations when using ATP Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.23 34300
-
 2 * 34300, SDS-PAGE Corynebacterium glutamicum
2.7.1.23 167500
-
 gel filtration Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.23 Corynebacterium glutamicum
-
-
-
2.7.1.23 Corynebacterium glutamicum ATCC 13032
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.23 ADP + NAD+
-
 Corynebacterium glutamicum AMP + NADP+
-
 ?
2.7.1.23 ADP + NAD+
-
 Corynebacterium glutamicum ATCC 13032 AMP + NADP+
-
 ?
2.7.1.23 ATP + NAD+ the catalytic efficiency with ATP as phosphate donor for phosphorylation of NAD is higher than with polyphosphate. The enzyme prefers ATP over ADP and polyphosphate(20) Corynebacterium glutamicum ADP + NADP+
-
 ?
2.7.1.23 ATP + NAD+ the catalytic efficiency with ATP as phosphate donor for phosphorylation of NAD is higher than with polyphosphate. The enzyme prefers ATP over ADP and polyphosphate(20) Corynebacterium glutamicum ATCC 13032 ADP + NADP+
-
 ?
2.7.1.23 polyphosphate(20) + NAD+
-
 Corynebacterium glutamicum polyphosphate(19) + NADP+
-
 ?
2.7.1.23 polyphosphate(20) + NAD+
-
 Corynebacterium glutamicum ATCC 13032 polyphosphate(19) + NADP+
-
 ?
2.7.1.23 polyphosphate(25) + NAD+
-
 Corynebacterium glutamicum polyphosphate(24) + NADP+
-
 ?
2.7.1.23 polyphosphate(25) + NAD+
-
 Corynebacterium glutamicum ATCC 13032 polyphosphate(24) + NADP+
-
 ?
2.7.1.23 polyphosphate(45) + NAD+
-
 Corynebacterium glutamicum polyphosphate(44) + NADP+
-
 ?
2.7.1.23 polyphosphate(45) + NAD+
-
 Corynebacterium glutamicum ATCC 13032 polyphosphate(44) + NADP+
-
 ?
2.7.1.23 polyphosphate(65) + NAD+
-
 Corynebacterium glutamicum polyphosphate(64) + NADP+
-
 ?
2.7.1.23 polyphosphate(75) + NAD+
-
 Corynebacterium glutamicum polyphosphate(74) + NADP+
-
 ?
2.7.1.23 tetraphosphate + NAD+
-
 Corynebacterium glutamicum triphosphate + NADP+
-
 ?
2.7.1.23 triphosphate + NAD+
-
 Corynebacterium glutamicum diphosphate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.23 homotetramer 2 * 34300, SDS-PAGE Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
2.7.1.23 polyP/ATP-dependent NAD kinase
-
 Corynebacterium glutamicum
2.7.1.23 Ppnk
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.23 55 65
-
 Corynebacterium glutamicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.23 45 60 preheating the enzyme for 1 h at temperatures up to 45°C does not alter the activity. When preheated at 50°C for 1 h, enzyme activity measured at 30°C decreases by 30% and preincubation at 56°C and 60°C leads to a fast and complete loss of activity after 10 and 5 min, respectively Corynebacterium glutamicum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.1.23 1.8
-
 polyphosphate(75) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 2.3
-
 ADP in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 3
-
 Triphosphate in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 3.4
-
 polyphosphate(45) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 3.4
-
 polyphosphate(65) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 7.3
-
 polyphosphate(25) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 7.8
-
 polyphosphate(20) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 7.8
-
 NAD+ in the presence of polyphosphate(20), in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 8.7
-
 Tetraphosphate in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 27.1
-
 NAD+ in the presence of ATP, in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 29.2
-
 ATP in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.23 7.1
-
 for polyphosphate-dependent activity Corynebacterium glutamicum
2.7.1.23 7.3
-
 for ATP-dependent activity Corynebacterium glutamicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.23 0.11
-
 NADH in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 0.34
-
 NADP+ in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.1.23 0.19
-
 polyphosphate(65) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 0.24
-
 polyphosphate(45) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 0.42
-
 polyphosphate(25) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 0.5
-
 ADP in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 0.58
-
 polyphosphate(75) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 1.2
-
 Triphosphate in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 1.3
-
 polyphosphate(20) in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 4.1
-
 Tetraphosphate in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 7.05
-
 NAD+ in the presence of polyphosphate(20), in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 40.5
-
 NAD+ in the presence of ATP, in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum
2.7.1.23 59.6
-
 ATP in 100 mM HEPES, pH 7.3, containing 20 mM MgCl2, at 30°C Corynebacterium glutamicum