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Literature summary extracted from

  • Robinson, P.; Neelon, K.; Schreier, H.J.; Roberts, M.F.
    beta-Glutamate as a substrate for glutamine synthetase (2001), Appl. Environ. Microbiol., 67, 4458-4463.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 overexpression in Escherichia coli Archaeoglobus fulgidus
6.3.1.2 overexpression in Escherichia coli Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 6
-
 ATP pH 7.0, 37°C Methanohalophilus portucalensis
6.3.1.2 58
-
 L-glutamate pH 7.0, 60°C Methanocaldococcus jannaschii
6.3.1.2 104
-
 L-glutamate pH 7.0, 37°C Methanohalophilus portucalensis
6.3.1.2 175
-
 3-aminopentanedioate pH 7.0, 37°C Methanohalophilus portucalensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mg2+ assay in presence of 55 mM Mg2+ Methanohalophilus portucalensis
6.3.1.2 Mg2+ assay in presence of 55 mM Mg2+ Archaeoglobus fulgidus
6.3.1.2 Mg2+ assay in presence of 55 mM Mg2+ Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Archaeoglobus fulgidus O29313
-
-
6.3.1.2 Archaeoglobus fulgidus VC-16 O29313
-
-
6.3.1.2 Methanocaldococcus jannaschii Q60182
-
-
6.3.1.2 Methanohalophilus portucalensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2
-
 Archaeoglobus fulgidus
6.3.1.2
-
 Methanocaldococcus jannaschii
6.3.1.2 partial Methanohalophilus portucalensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.1.2 7.3
-
 pH 7.0, 37°C Methanohalophilus portucalensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + 3-aminopentanedioate + hydroxylamine the activity with is 3-aminopentanedioate (beta-glutamate) is 7fold less than the rate obtained with alpha-glutamate Methanohalophilus portucalensis ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + 3-aminopentanedioate + hydroxylamine the enzyme is more selective for L-glutamate (alpha-glutamate) than for 3-aminopentanedioate (beta-glutamate) as a substrate Archaeoglobus fulgidus ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + 3-aminopentanedioate + hydroxylamine the enzyme is more selective for L-glutamate (alpha-glutamate) than for 3-aminopentanedioate (beta-glutamate) as a substrate Archaeoglobus fulgidus VC-16 ADP + phosphate + ?
-
 ?
6.3.1.2 ATP + 3-aminopentanedioate + hydroxylamine the enzyme is more selective for L-glutamate (alpha-glutamate) than 3-aminopentanedioate (beta-glutamate) as a substrate Methanocaldococcus jannaschii ADP + phosphate + gamma-glutamylhydroxamate
-
 ?
6.3.1.2 ATP + L-glutamate + hydroxylamine the activity with L-glutamate is 7fold higher than the rate obtained with 3-aminopentanedioate (beta-glutamate) Methanohalophilus portucalensis ADP + phosphate + gamma-L-glutamylhydroxamate
-
 ?
6.3.1.2 ATP + L-glutamate + hydroxylamine the enzyme is more selective for L-glutamate (alpha-glutamate) than for 3-aminopentanedioate (beta-glutamate) as a substrate Archaeoglobus fulgidus ADP + phosphate + gamma-L-glutamylhydroxamate
-
 ?
6.3.1.2 ATP + L-glutamate + hydroxylamine the enzyme is more selective for L-glutamate (alpha-glutamate) than for 3-aminopentanedioate (beta-glutamate) as a substrate Methanocaldococcus jannaschii ADP + phosphate + gamma-L-glutamylhydroxamate
-
 ?
6.3.1.2 ATP + L-glutamate + hydroxylamine the enzyme is more selective for L-glutamate (alpha-glutamate) than for 3-aminopentanedioate (beta-glutamate) as a substrate Archaeoglobus fulgidus VC-16 ADP + phosphate + gamma-L-glutamylhydroxamate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.3.1.2 GlnA
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 37
-
 assay at Methanohalophilus portucalensis
6.3.1.2 60
-
 assay at Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 7
-
 assay at Methanohalophilus portucalensis
6.3.1.2 7
-
 assay at Archaeoglobus fulgidus
6.3.1.2 7
-
 assay at Methanocaldococcus jannaschii