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Literature summary extracted from

  • Sucipto, H.; Kudo, F.; Eguchi, T.
    The last step of kanamycin biosynthesis: unique deamination reaction catalyzed by the alpha-ketoglutarate-dependent nonheme iron dioxygenase KanJ and the NADPH-dependent reductase KanK (2012), Angew. Chem. Int. Ed. Engl., 51, 3428-3431.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.11.37 2-oxoglutarate dependent on Streptomyces kanamyceticus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.355 expression in Escherichia coli Streptomyces kanamyceticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.11.37 Fe2+ the enzyme is dependent on exogenous ferrous ions, although residual activity is also observed in the absence of exogenous Fe2+ Streptomyces kanamyceticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.355 2'-dehydrokanamycin A + NADPH + H+ Streptomyces kanamyceticus the enzyme is involved in the conversion of kanamycin B to kanamycin A. Kanamycin is a clinically important aminoglycoside antibiotic kanamycin A + NADP+
-
 ?
1.14.11.37 kanamycin B + 2-oxoglutarate + O2 Streptomyces kanamyceticus
-
 2'-dehydrokanamycin A + succinate + NH3 + CO2
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.355 Streptomyces kanamyceticus Q2MFU6
-
-
1.14.11.37 Streptomyces kanamyceticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.355
-
 Streptomyces kanamyceticus
1.14.11.37 nickel-affinity column chromatography Streptomyces kanamyceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.355 2'-dehydrokanamycin A + NADPH + H+ the enzyme is involved in the conversion of kanamycin B to kanamycin A. Kanamycin is a clinically important aminoglycoside antibiotic Streptomyces kanamyceticus kanamycin A + NADP+
-
 ?
1.1.1.355 2'-dehydrokanamycin A + NADPH + H+ i.e. (1S,2R,3R,4S,6R)-4,6-diamino-3-(6-amino-6-deoxy-a-D-glucopyranosyloxy)-2-hydroxycyclohexyl 3-amino-3-deoxy-alpha-D-glucopyranoside Streptomyces kanamyceticus kanamycin A + NADP+ i.e. (1S,2R,3R,4S,6R)-4,6-diamino-3-[(6-amino-6-deoxy-a-D-arabino-hexopyranosyl-2-ulose)oxy]-2-hydroxycyclohexyl 3-amino-3-deoxy-alpha-D-glucopyranoside ?
1.14.11.37 kanamycin B + 2-oxoglutarate + O2
-
 Streptomyces kanamyceticus 2'-dehydrokanamycin A + succinate + NH3 + CO2
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.355 kanK
-
 Streptomyces kanamyceticus
1.14.11.37 kanJ
-
 Streptomyces kanamyceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.355 NADPH
-
 Streptomyces kanamyceticus