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Literature summary extracted from

  • Sharma, A.; Nair, D.T.
    Cloning, expression, purification, crystallization and preliminary crystallographic analysis of MsDpo4: a Y-family DNA polymerase from Mycobacterium smegmatis (2011), Acta Crystallogr. Sect. F, 67, 812-816.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7 Rv1537 and Rv3056, expression of GST-tagged MsDpo4 in Escherichia coli strain C41(DE3) Mycolicibacterium smegmatis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.7 purified native and selenomethionine-labeled MsDpo4, hanging drop vapour diffusion method, 1 mM protein is mmixed with 2.4 M NaCl, and 0.1 M sodium/potassium phosphate, pH 6.4, 72 h, 25°C, optimization of crystallization and cryoprotection method, X-ray diffraction structure determination and analysis at 26 A resolution, molecular replacement Mycolicibacterium smegmatis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.7.7 39146
-
 x * 39146, purified recombinant detagged enzyme, mass spectrometry Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Mycolicibacterium smegmatis A0QR77 two family Y DNA polymerases Rv1537 and Rv3056
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7 GST-tagged MsDpo4 from Escherichia coli strain C41(DE3) by glutathione affinity chromatography, the tag is cleaved off Mycolicibacterium smegmatis

Subunits

EC Number Subunits Comment Organism
2.7.7.7 ? x * 39146, purified recombinant detagged enzyme, mass spectrometry Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
2.7.7.7 DNA polymerase IV
-
 Mycolicibacterium smegmatis
2.7.7.7 MsDpo4
-
 Mycolicibacterium smegmatis
2.7.7.7 Rv1537
-
 Mycolicibacterium smegmatis
2.7.7.7 Rv3056
-
 Mycolicibacterium smegmatis

Expression

EC Number Organism Comment Expression
2.7.7.7 Mycolicibacterium smegmatis the expression of Rv1537 and Rv3056 is not upregulated under conditions of stress additional information

General Information

EC Number General Information Comment Organism
2.7.7.7 physiological function error-prone DNA polymerases belonging to the Y-family in mycobacteria do not participate in adaptive mutagenesis, in contrast to the representatives of theis faamily in other prokaryotes. Escherichia coli gene dinB homologues in mycobacteria code for nonfunctional molecules that are devoid of enzyme activity Mycolicibacterium smegmatis