EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.28 | gene APE_0487, expression in Escherichia coli strain Rosetta-gami 2 (DE3) | Aeropyrum pernix |
1.1.99.6 | expression in Escherichia coli | Aeropyrum pernix |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.1.1.28 | purified recombinant enzyme, hanging drop vapour diffusion method, mixing of 0.002 ml of 15 mg/ml protein in 10 mM potassium phosphate, pH 7.0, with 0.002 ml of reservoir solution containing 14.4% PEG 8000, 80 mM cacodylate pH 6.5, 160 mM calcium acetate and 20% glycerol, and equilibration against 0.1 ml of reservoir solution, 25°C, 2 weeks, X-ray diffraction structure determination and analysis at 2.0 A resolution | Aeropyrum pernix |
1.1.99.6 | hanging-drop vapour-diffusion method with polyethylene glycol 8000 as the precipitant. The crystals belong to the monoclinic space group P2(1), with unit-cell parameters a = 63.4, b = 119.4, c = 70.2 A, beta = 112.0°, and diffract to 2.0 A resolution | Aeropyrum pernix |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.28 | 59000 | - |
1 * 59000, recombinant enzyme, SDS-PAGE | Aeropyrum pernix |
1.1.1.28 | 61000 | - |
recombinant enzyme, gel filtration | Aeropyrum pernix |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.28 | (R)-lactate + NAD+ | Aeropyrum pernix | - |
pyruvate + NADH + H+ | - |
r | |
1.1.1.28 | (R)-lactate + NAD+ | Aeropyrum pernix DSM 11879 | - |
pyruvate + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.28 | Aeropyrum pernix | Q9YEU4 | gene APE_0487; gene APE_0487 | - |
1.1.99.6 | Aeropyrum pernix | Q9YEU4 | - |
- |
1.1.99.6 | Aeropyrum pernix DSM 11879 | Q9YEU4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.28 | recombinant enzyme from Escherichia coli strain Rosetta-gami 2 (DE3) by nickel affnity chromatography and gel filtration | Aeropyrum pernix |
1.1.99.6 | - |
Aeropyrum pernix |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.28 | (R)-lactate + NAD+ | - |
Aeropyrum pernix | pyruvate + NADH + H+ | - |
r | |
1.1.1.28 | (R)-lactate + NAD+ | - |
Aeropyrum pernix DSM 11879 | pyruvate + NADH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.28 | monomer | 1 * 59000, recombinant enzyme, SDS-PAGE | Aeropyrum pernix |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.28 | DLDH | - |
Aeropyrum pernix |
1.1.99.6 | APE_0487 | - |
Aeropyrum pernix |
1.1.99.6 | dye-linked D-lactate dehydrogenase | - |
Aeropyrum pernix |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.28 | NAD+ | - |
Aeropyrum pernix | |
1.1.1.28 | NADH | - |
Aeropyrum pernix |