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Literature summary extracted from

  • Chhabra, S.; Newman, J.; Peat, T.S.; Fernley, R.T.; Caine, J.; Simpson, J.S.; Swarbrick, J.D.
    Crystallization and preliminary X-ray analysis of 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase from Staphylococcus aureus (2010), Acta Crystallogr. Sect. F, 66, 575-578.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.6.3 expressed in Escherichia coli BL21(DE3) cells Staphylococcus aureus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.6.3 sitting drop vapor diffusion method, using 1.08 M sodium malonate pH 7, 0.09 M bis-Tris pH 6.5, 0.175 M sodium formate, and 0.01 M sodium acetate pH 4.6 Staphylococcus aureus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.6.3 Mg2+ dependent on Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
2.7.6.3 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.6.3 Ni-NTA column chromatography and Superdex 75 gel filtration Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.6.3 ATP + 6-hydroxymethyl-7,8-dihydropteridine
-
 Staphylococcus aureus AMP + 6-hydroxymethyl-7,8-dihydropteridine diphosphate
-
 ir

Synonyms

EC Number Synonyms Comment Organism
2.7.6.3 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase
-
 Staphylococcus aureus
2.7.6.3 HPPK
-
 Staphylococcus aureus

General Information

EC Number General Information Comment Organism
2.7.6.3 metabolism 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinae is a committed enzyme in the folate pathway of Streptococcus aureus playing an integral role in the biosynthesis of tetrahydrofolate Staphylococcus aureus