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Literature summary extracted from

  • Brito, J.A.; Borges, N.; Santos, H.; Archer, M.
    Production, crystallization and preliminary X-ray analysis of CTP:inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus (2010), Acta Crystallogr. Sect. F, 66, 1463-1465.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.74 the CTP:inositol-1-phosphate cytidylyltransferase domain of the bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase (EC 2.7.7.74/EC 2.7.8.34) is cloned and expressed in Escherichia coli Archaeoglobus fulgidus
2.7.8.34 expressed in Escherichia coli BL21(DE3)pLyS cells Archaeoglobus fulgidus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.7.74 crystallization of the CTP:inositol-1-phosphate cytidylyltransferase domain in the apo form. The crystals diffracted to 2.4 A resolution using a synchrotron source and belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 154.7, b = 83.9, c = 127.7 A Archaeoglobus fulgidus
2.7.8.34 apo enzyme, sitting drop vapor diffusion method, using 1.2-1.4 M trisodium citrate dihydrate, 100 mM HEPES pH 6.8-7.2 and 10 mM MgCl2 Archaeoglobus fulgidus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.8.34 soluble
-
 Archaeoglobus fulgidus
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.74 Archaeoglobus fulgidus O29976 CTP:inositol-1-phosphate cytidylyltransferase domain of the bifunctional enzyme CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase (EC 2.7.7.74/EC 2.7.8.34)
-
2.7.8.34 Archaeoglobus fulgidus
-
-
-
2.7.8.34 Archaeoglobus fulgidus AF0263
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.74 purification CTP:inositol-1-phosphate cytidylyltransferase domain Archaeoglobus fulgidus
2.7.8.34 His-Trap column chromatography and Superdex G75 gel filtration Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.8.34 CDP-1L-myo-inositol + 1L-myo-inositol 1-phosphate
-
 Archaeoglobus fulgidus CMP + bis(1L-myo-inositol) 3,1'-phosphate 1-phosphate
-
 ?
2.7.8.34 CDP-1L-myo-inositol + 1L-myo-inositol 1-phosphate
-
 Archaeoglobus fulgidus AF0263 CMP + bis(1L-myo-inositol) 3,1'-phosphate 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.74 CTP:inositol-1-phosphate cytidylyltransferase
-
 Archaeoglobus fulgidus
2.7.7.74 IPCT
-
 Archaeoglobus fulgidus
2.7.8.34 CTP:inositol-1-phosphate cytidylyltransferase bifunctional enzyme Archaeoglobus fulgidus
2.7.8.34 IPCT
-
 Archaeoglobus fulgidus

General Information

EC Number General Information Comment Organism
2.7.7.74 physiological function the bifunctional CTP:inositol-1-phosphate cytidylyltransferase/CDP-inositol:inositol-1-phosphate transferase (EC 2.7.7.74/EC 2.7.8.34) is involved in biosynthesis of bis(1L-myo-inositol) 1,3-phosphate Archaeoglobus fulgidus