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Literature summary extracted from

  • Lambrecht, J.A.; Downs, D.M.
    Anthranilate phosphoribosyl transferase (TrpD) generates phosphoribosylamine for thiamine synthesis from enamines and phosphoribosyl pyrophosphate (2013), ACS Chem. Biol., 8, 242-248.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.18 His-tagged protein expressed in Escherichia coli Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.18 D282A mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine, mutant enzyme does synthesize sufficient tryptophan to allow growth (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica
2.4.2.18 G308L mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine, mutant enzyme does synthesize sufficient tryptophan to allow growth (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica
2.4.2.18 H307A mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine, mutant enzyme does synthesize sufficient tryptophan to allow growth (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica
2.4.2.18 K306A mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica
2.4.2.18 N309A mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine, mutant enzyme does synthesize sufficient tryptophan to allow growth (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica
2.4.2.18 R364A mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica
2.4.2.18 T279A mutant enzyme does not generate sufficient phosphoribosyl amine to allow growth in the absence of thiamine, mutant enzyme does synthesize sufficient tryptophan to allow growth (in vivo analysis using the Salmonella enterica strain DM9813 (purF gnd ridA trpD, auxotrophic requirement for purines, thiamine and tryptophan)) Salmonella enterica

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.18 Salmonella enterica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.18 immobilized metal ion affinity chromatography (Ni2+) Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.18 2-aminocrotonate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Salmonella enterica N-(5-phospho-D-ribosyl)-2-aminocrotonate + diphosphate reaction product predicted to be a phosphoribosyl-enamine adduct, which breaks down directly into phosphoribosyl amine and 2-ketobutyrate (a reaction mediated by TrpD or non-enzymatic), or which breaks down non-enzymatically into ribose 5'-phosphate, 2-ketobutyrate and NH3. Ribose 5'-phosphate and NH3 combine non-enzymatically to form phosphoribosyl amine ?
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate also 4- and 5-carbon enamines (2-aminocrotonate, 2-amino-2-pentenoate, also 2-aminoacrylate) serve as substrates, 2-aminobutyrate is not used as substrate Salmonella enterica N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.18 anthranilate phosphoribosyl transferase bifunctional enzyme EC 4.1.3.27, 2.4.2.18 Salmonella enterica
2.4.2.18 Trp D
-
 Salmonella enterica

General Information

EC Number General Information Comment Organism
2.4.2.18 physiological function tryptophan biosynthetic pathway Salmonella enterica