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Literature summary extracted from

  • Chatterjee, K.; Blaby, I.K.; Thiaville, P.C.; Majumder, M.; Grosjean, H.; Yuan, Y.A.; Gupta, R.; de Crecy-Lagard, V.
    The archaeal COG1901/DUF358 SPOUT-methyltransferase members, together with pseudouridine synthase Pus10, catalyze the formation of 1-methylpseudouridine at position 54 of tRNA (2012), RNA, 18, 421-433.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.257 additional information Methanocaldococcus jannaschii TrmY requires C55 (or possibly U55) in the tRNA to produce m1PSI54 Methanocaldococcus jannaschii

Application

EC Number Application Comment Organism
5.4.99.B25 molecular biology using the COG1901 recombinant enzyme from Methanocaldococcus jannaschii (Mj1640), purified enzyme Pus10 from Methanocaldococcus jannaschii and full-size tRNA transcripts or T(PSI)-arm (17-mer) fragments as substrates, the sequential pathway of m(1)PSI54 formation in Archaea is reconstituted Methanocaldococcus jannaschii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.257 gene trmY or HVO_1989 Haloferax volcanii
2.1.1.257 gene VNG1980C, expression of the gene encoding COG1901 from Halobacterium sp. strain NRC-1 in Haloferax volcanii strain DHVO_1989 complements the m1PSI minus, enzyme-deficient phenotype of the mutant strain Halobacterium sp.

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.257 additional information expression of the gene VNG1980C encoding COG1901 from Halobacterium sp. strain NRC-1 in Haloferax volcanii strain DHVO_1989 complements the m1PSI minus, enzyme-deficient phenotype of the mutant strain Haloferax volcanii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.257 S-adenosyl-L-homocysteine
-
 Halobacterium sp.
2.1.1.257 S-adenosyl-L-homocysteine
-
 Haloferax volcanii
2.1.1.257 S-adenosyl-L-homocysteine
-
 Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in tRNA Haloferax volcanii
-
 S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
 ?
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in tRNA Halobacterium sp.
-
 S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
 ?
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in tRNA Methanocaldococcus jannaschii
-
 S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
 ?
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in tRNA Halobacterium sp. NRC-1
-
 S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
 ?
5.4.99.B25 tRNA uridine54 Methanocaldococcus jannaschii
-
 tRNA pseudouridine54
-
 ?
5.4.99.B25 tRNA uridine54 Methanocaldococcus jannaschii DSM 2661
-
 tRNA pseudouridine54
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.257 Halobacterium sp. Q9HNR6 gene VNG1980C
-
2.1.1.257 Halobacterium sp. NRC-1 Q9HNR6 gene VNG1980C
-
2.1.1.257 Haloferax volcanii D4GTL8 gene HVO_1989 or trmY
-
2.1.1.257 Methanocaldococcus jannaschii Q59034 gene Mj1640
-
5.4.99.B25 Methanocaldococcus jannaschii Q60346
-
-
5.4.99.B25 Methanocaldococcus jannaschii DSM 2661 Q60346
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.257 additional information enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview Haloferax volcanii ?
-
 ?
2.1.1.257 additional information enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview Halobacterium sp. ?
-
 ?
2.1.1.257 additional information enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview Methanocaldococcus jannaschii ?
-
 ?
2.1.1.257 additional information enzymatic post-transcriptional modifications of selected uridines in tRNA, secondary structure, U54 targets within the highly conserved 7-nt TPSI-loop, overview Halobacterium sp. NRC-1 ?
-
 ?
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in elongator tRNAMet
-
 Haloferax volcanii S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in elongator tRNAMet
-
 ?
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in tRNA
-
 Haloferax volcanii S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
 ?
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in tRNA
-
 Halobacterium sp. S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
 ?
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in tRNA
-
 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
 ?
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in tRNA methylation of pseudouridine PSI at position 54 of tRNA, in vitro and in vivo reaction, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
 ?
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in tRNA methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate Haloferax volcanii S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
 ?
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in tRNA methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate Halobacterium sp. S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
 ?
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in tRNA
-
 Halobacterium sp. NRC-1 S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
 ?
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in tRNA methylation of pseudouridine PSI at position 54 of tRNA, producing m1PSI, in vitro pseudouridylated tRNATrp is used as a substrate Halobacterium sp. NRC-1 S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNA
-
 ?
2.1.1.257 S-adenosyl-L-methionine + pseudouridine54 in tRNATrp
-
 Haloferax volcanii S-adenosyl-L-homocysteine + N1-methylpseudouridine54 in tRNATrp
-
 ?
5.4.99.B25 tRNA uridine54
-
 Methanocaldococcus jannaschii tRNA pseudouridine54
-
 ?
5.4.99.B25 tRNA uridine54
-
 Methanocaldococcus jannaschii DSM 2661 tRNA pseudouridine54
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.257 COG1901
-
 Haloferax volcanii
2.1.1.257 COG1901
-
 Halobacterium sp.
2.1.1.257 COG1901
-
 Methanocaldococcus jannaschii
2.1.1.257 DUF358
-
 Haloferax volcanii
2.1.1.257 DUF358
-
 Halobacterium sp.
2.1.1.257 DUF358
-
 Methanocaldococcus jannaschii
2.1.1.257 SAM-dependent N1-pseudouridine methyltransferase
-
 Methanocaldococcus jannaschii
2.1.1.257 TrmY protein
-
 Haloferax volcanii
2.1.1.257 TrmY protein
-
 Halobacterium sp.
2.1.1.257 TrmY protein
-
 Methanocaldococcus jannaschii
5.4.99.B25 Pus10
-
 Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
2.1.1.257 evolution m1PSI formation in archaeal tRNAs, overview. The enzyme belongs to Cluster of Orthologous Group, COG, 1901 Haloferax volcanii
2.1.1.257 evolution m1PSI formation in archaeal tRNAs, overview. The enzyme belongs to Cluster of Orthologous Group, COG, 1901 Halobacterium sp.
2.1.1.257 evolution m1PSI formation in archaeal tRNAs, overview. The enzyme belongs to Cluster of Orthologous Group, COG, 1901 Methanocaldococcus jannaschii
2.1.1.257 malfunction deletion of trmY leads to the absence of m1PSI54 in Haloferax volcanii tRNA Haloferax volcanii
2.1.1.257 metabolism Pus10-mediated PSI54 (and PSI55) formation does not require the presence of TrmY activity, overview Haloferax volcanii
2.1.1.257 metabolism Pus10-mediated PSI54 (and PSI55) formation does not require the presence of TrmY activity, overview Methanocaldococcus jannaschii
2.1.1.257 additional information Methanocaldococcus jannaschii TrmY requires C55 (or possibly U55) in the tRNA to produce m1PSI54 Methanocaldococcus jannaschii
2.1.1.257 physiological function trmY is not essential in Haloferax volcanii Haloferax volcanii