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Literature summary extracted from

  • Benitez-Paez, A.; Villarroya, M.; Armengod, M.E.
    The Escherichia coli RlmN methyltransferase is a dual-specificity enzyme that modifies both rRNA and tRNA and controls translational accuracy (2012), RNA, 18, 1783-1795.
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.192 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Escherichia coli
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 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
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 ?
2.1.1.192 additional information Escherichia coli RlmN introduces m2A at position 2503 in the peptidyl transferase center of 23S RNA. RlmN is a dual-specificity enzyme that catalyzes methylation of both rRNA and tRNA, see for EC 2.1.1.66, substrate specificity analysis, overview ?
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Organism

EC Number Organism UniProt Comment Textmining
2.1.1.192 Escherichia coli
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.192 2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
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 Escherichia coli S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
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 ?
2.1.1.192 additional information RlmN introduces m2A at position 2503 in the peptidyl transferase center of 23S RNA. RlmN is a dual-specificity enzyme that catalyzes methylation of both rRNA and tRNA, see for EC 2.1.1.66, substrate specificity analysis, overview Escherichia coli ?
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 ?
2.1.1.192 additional information recombinant His-RlmN protein is able to catalyze the synthesis of m2A in a SAM-dependent manner on tRNAchimera UUG purified from DELTArlmN cells Escherichia coli ?
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Synonyms

EC Number Synonyms Comment Organism
2.1.1.192 radical S-adenosyl-L-methionine enzyme
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 Escherichia coli
2.1.1.192 radical SAM enzyme
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 Escherichia coli
2.1.1.192 RlmN
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 Escherichia coli
2.1.1.192 RlmN methyltransferase
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 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.192 S-adenosyl-L-methionine dependent on Escherichia coli

General Information

EC Number General Information Comment Organism
2.1.1.192 malfunction the DrlmN mutant lacks m2A in both RNA types, whereas the expression of recombinant RlmN from a plasmid introduced into this mutant restores tRNA modification. RlmN inactivation increases the misreading of a UAG stop codon. Since loss of m2A37 from tRNA is expected to produce a hyperaccurate phenotype, the error-prone phenotype exhibited by the DrlmN mutant is due to loss of m2A from 23S rRNA Escherichia coli
2.1.1.192 physiological function RlmN introduces m2A at position 2503 in the peptidyl transferase center of 23S RNA. RlmN is a dual-specificity enzyme that catalyzes methylation of both rRNA and tRNA. RlmN works in a late step during tRNA maturation by recognizing a precise 3D structure of tRNA, see for EC 2.1.1.66. The m2A2503 modification plays a crucial role in the proofreading step occurring at the peptidyl transferase center Escherichia coli