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Literature summary extracted from

  • Christian, T.; Lahoud, G.; Liu, C.; Hoffmann, K.; Perona, J.J.; Hou, Y.M.
    Mechanism of N-methylation by the tRNA m1G37 methyltransferase Trm5 (2010), RNA, 16, 2484-2492.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.228 analysis of the crystal structure of the Trm5-tRNA-AdoMet ternary complex Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.228 D223A site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 D223E site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 D223L site-directed mutagenesis, the mutant shows complete loss of activity Methanocaldococcus jannaschii
2.1.1.228 D223N site-directed mutagenesis, the mutant shows complete loss of activity Methanocaldococcus jannaschii
2.1.1.228 E185A site-directed mutagenesis, the mutant shows complete loss of activity Methanocaldococcus jannaschii
2.1.1.228 E185D site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 E185Q site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 K137A site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 K318A site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 N265A site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 N265H site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 N265Q site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 P267A site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 R145A site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 R181A site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 R186A site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 Y177A site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii
2.1.1.228 Y177F site-directed mutagenesis, the mutant shows altered single turnover kinetics compared to the wild-type enzyme Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.228 additional information
-
 additional information wild-type and mutant enzymes pH-dependence of the single-turnover rate constant: the pH dependence of kobs in single-turnover analysis corresponds to proton ransfer during a slower process of induced fit, rather than the bond-breaking and bond-forming steps of methyl transfer, detailed analysis and overview Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA Methanocaldococcus jannaschii
-
 S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.228 Methanocaldococcus jannaschii Q58293
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA mechanism of N-methylation by the tRNA m1G37 methyltransferase Trm5 involving proton abstraction during docking of G37 in the active site by a general base , E185 Methanocaldococcus jannaschii
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.228 additional information 85°C is the optimal growth temperature Methanocaldococcus jannaschii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.228 additional information structure of Trm5 active site bound to tRNA and S-adenosyl-L-methionine, induced fit for active-site assembly, detailed overview. E185 is crucial both for general base catalysis and for the conformational change that precedes catalysis Methanocaldococcus jannaschii ?
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNA
-
 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in tRNACys Methanococcus jannaschii tRNACys Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACys
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.228 TRM5
-
 Methanocaldococcus jannaschii
2.1.1.228 tRNA m1G37 methyltransferase
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.228 55
-
 assay at Methanocaldococcus jannaschii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.228 6 9.8 preincubation of Trm5 at pH 6.0, followed by adjustment of the solution to pH 8.0, does not cause irreversible inactivation of the enzyme. Enzyme treated in this manner retained equivalent activity in single-turnover assay Methanocaldococcus jannaschii

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.228 S-adenosyl-L-methionine
-
 Methanocaldococcus jannaschii