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Literature summary extracted from

  • Wang, K.; Wang, Q.; Chen, J.; Chen, L.; Jiang, H.; Shen, X.
    Crystal structure and enzymatic characterization of thymidylate synthase X from Helicobacter pylori strain SS1 (2011), Protein Sci., 20, 1398-1410.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.1.1.148 NADPH 0.1 mM NADPH saturates the enzymatic reaction Helicobacter pylori

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.148 expressed in Escherichia coli M15 cells Helicobacter pylori

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.148 hanging drop vapor diffusion method, using 0.2 M sodium chloride, 0.1 M MES, pH 6.0, and 20% (w/v) PEG2000 monomethyl ether Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.45 10-methylfolate
-
 Helicobacter pylori
2.1.1.45 5-fluoro-2'-deoxyuridine monophosphate
-
 Helicobacter pylori
2.1.1.45 8-azapurinone deoxyribonucleoside 5'-phosphate
-
 Helicobacter pylori
2.1.1.45 N-(4-{[(2-amino-4-oxo-1,4-dihydroquinazolin-6-yl)methyl](prop-2-ynoyl)amino}benzoyl)-L-glutamic acid
-
 Helicobacter pylori
2.1.1.148 NADPH excess NADPH decreases enzyme activity Helicobacter pylori

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.148 0.0153
-
 dUMP in 50 mM Tris-HCl pH 8.0, 10 mM MgCl2, 2 mM NADPH, 0.5 mM FAD, at 22°C Helicobacter pylori
2.1.1.148 0.35
-
 5,10-methylenetetrahydrofolate in 50 mM Tris-HCl pH 8.0, 10 mM MgCl2, 2 mM NADPH, 0.5 mM FAD, at 22°C Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.148 25000
-
 4 * 25000, SDS-PAGE Helicobacter pylori
2.1.1.148 25020
-
 4 * 25020, calculated from amino acid sequence Helicobacter pylori
2.1.1.148 25066
-
 4 * 25066, MALDI TOF mass spectrometry Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.45 Helicobacter pylori
-
-
-
2.1.1.45 Helicobacter pylori SS1
-
-
-
2.1.1.148 Helicobacter pylori Q5UVJ4
-
-
2.1.1.148 Helicobacter pylori SS1 Q5UVJ4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.148 nickel chelating column chromatography, GSH-Sepharose column chromatography, and Superdex 200 gel filtration Helicobacter pylori

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Helicobacter pylori dihydrofolate + dTMP
-
 ?
2.1.1.45 5,10-methylenetetrahydrofolate + dUMP
-
 Helicobacter pylori SS1 dihydrofolate + dTMP
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Helicobacter pylori dTMP + tetrahydrofolate + FAD
-
 ?
2.1.1.148 5,10-methylenetetrahydrofolate + dUMP + FADH2
-
 Helicobacter pylori SS1 dTMP + tetrahydrofolate + FAD
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.148 homotetramer 4 * 25000, SDS-PAGE Helicobacter pylori
2.1.1.148 homotetramer 4 * 25020, calculated from amino acid sequence Helicobacter pylori
2.1.1.148 homotetramer 4 * 25066, MALDI TOF mass spectrometry Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
2.1.1.45 ThyA
-
 Helicobacter pylori
2.1.1.45 thymidylate synthase A
-
 Helicobacter pylori
2.1.1.148 thymidylate synthase X
-
 Helicobacter pylori
2.1.1.148 ThyX
-
 Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.148 8.5
-
-
 Helicobacter pylori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.148 6.5 10.5
-
 Helicobacter pylori

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.1.148 FAD the enzyme is bound to 4 FAD molecules Helicobacter pylori