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Literature summary extracted from

  • Pumplin, N.; Zhang, X.; Noar, R.D.; Harrison, M.J.
    Polar localization of a symbiosis-specific phosphate transporter is mediated by a transient reorientation of secretion (2012), Proc. Natl. Acad. Sci. USA, 109, E665-E672.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.3.2.1 MtPT4-GFP and MtPT1 expression from the MtPT4 and MtPT1 promoter, respectively, in Medicago truncatula plants in the periarbuscular membrane, and from the 35S promoter in roots and in the vacuole of vascular tissue cells. The plasma membrane GFP signal appears substantially weaker in cells harboring arbuscules than in other cortical cells. MtPT1-GFP fusions were expressed from the MtBcp1 promoter, which is active in cortical cells both before and during arbuscule formation. Phenotype of roots expressing pMtBcp1:MtPT4-GFP, overview Medicago truncatula

Protein Variants

EC Number Protein Variants Comment Organism
7.3.2.1 S115F mutant MtPT4S115F is retained in the endomembrane system and colocalizes with endoplasmic reticulum and trans-Golgi network markers. Mutation of this residue disrupts a conserved process in phosphate transporter trafficking Medicago truncatula
7.3.2.1 S117F mutant MtPT1S117F is retained in the endomembrane system and colocalizes with endoplasmic reticulum and trans-Golgi network markers. Mutation of this residue disrupts a conserved process in phosphate transporter trafficking Medicago truncatula

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.3.2.1 additional information during arbuscule formation, secretion of newly synthesized phosphate transporters is directed predominantly toward the developing periarbuscular membrane and not toward the plasma membrane. Subcellular enzyme expression patterns, overview. Correct localization of MtPT4 is regulated by a polarization of the bulk secretory pathway favoring vesicle fusion with the developing periarbuscular membrane rather than with the plasma membrane, and that there is a coincident change in the newly synthesized protein cargo entering into the secretion system Medicago truncatula
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7.3.2.1 periarbuscular membrane MtPT4 and MtPT1, physically continuous with the plasma membrane of the cortical cell, MtPT4 is located only in the domain around the arbuscule branches, targeting of MtPT4 to the periarbuscular membrane, mechanism by precise temporal expression coupled with a transient reorientation of secretion and alterations in the protein cargo entering the secretory system of the colonized root cell, overview Medicago truncatula 85042
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Organism

EC Number Organism UniProt Comment Textmining
7.3.2.1 Medicago truncatula
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 cv. Jemalong
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7.3.2.1 Medicago truncatula A17
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 cv. Jemalong
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Synonyms

EC Number Synonyms Comment Organism
7.3.2.1 mTPT1
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 Medicago truncatula
7.3.2.1 MtPT4
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 Medicago truncatula
7.3.2.1 symbiosis-specific phosphate transporter
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 Medicago truncatula

General Information

EC Number General Information Comment Organism
7.3.2.1 metabolism the enzyme is involved in arbuscular mycorrhizal, AM, symbiosis,. During arbuscular mycorrhizal symbiosis, the AM fungus colonizes the root cortical cells where it forms branched hyphae called arbuscules that function in nutrient exchange with the plant. Each arbuscule is enveloped in a plant membrane, the periarbuscular membrane, that contains a unique set of proteins including phosphate transporters such as MtPT4 that are essential for symbiotic phosphate transport Medicago truncatula