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Literature summary extracted from
- In vitro assembly of the undecaprenylpyrophosphate-linked heptasaccharide for prokaryotic N-linked glycosylation (2005), Proc. Natl. Acad. Sci. USA, 102, 14255-14259.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.293 | additional information | PglI prefers native lipids for optimal activity | Campylobacter jejuni |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.289 | gene pglH, overexpression of His-tagged PglA in Escherichia coli | Campylobacter jejuni |
| 2.4.1.290 | gene pglA, cloning and overexpression of glycosyltransferase PglA as His-tagged enzyme in Escherichia coli | Campylobacter jejuni |
| 2.4.1.291 | gene pglJ, overexpression of His-tagged PglJ in Escherichia coli | Campylobacter jejuni |
| 2.4.1.292 | gene pglH, overexpression of His-tagged PglH in Escherichia coli | Campylobacter jejuni |
| 2.4.1.293 | gene pglI, overexpression of His-tagged PglI in Escherichia coli | Campylobacter jejuni |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 2.4.1.291 | membrane | asociated | Campylobacter jejuni | 16020 | - |
| 2.4.1.292 | membrane | asociated | Campylobacter jejuni | 16020 | - |
| 2.4.1.293 | membrane | - |
Campylobacter jejuni | 16020 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.289 | 43000 | - |
x * 43000, recombinant His-tagged PglA, SDS-PAGE | Campylobacter jejuni |
| 2.4.1.290 | 43000 | - |
x * 43000, recombinant His-tagged PglA, SDS-PAGE | Campylobacter jejuni |
| 2.4.1.291 | 41000 | - |
x * 41000, recombinant His-tagged PglJ, SDS-PAGE | Campylobacter jejuni |
| 2.4.1.292 | 41000 | - |
x * 41000, recombinant His-tagged PglH, SDS-PAGE | Campylobacter jejuni |
| 2.4.1.293 | 36000 | - |
x * 36000, recombinant His-tagged Pgli, SDS-PAGE | Campylobacter jejuni |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.289 | dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | Campylobacter jejuni | - |
dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? |  |
| 2.4.1.289 | dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | Campylobacter jejuni NCTC 11168 | - |
dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
| 2.4.1.290 | UDP-N-acetyl-alpha-D-galactosamine + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | Campylobacter jejuni | - |
UDP + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
| 2.4.1.290 | UDP-N-acetyl-alpha-D-galactosamine + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | Campylobacter jejuni NCTC 11168 | - |
UDP + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
| 2.4.1.291 | UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | Campylobacter jejuni | - |
UDP + N-acetyl-D-galactosaminyl-alpha-(1->4)-N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
| 2.4.1.291 | UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | Campylobacter jejuni NCTC 11168 | - |
UDP + N-acetyl-D-galactosaminyl-alpha-(1->4)-N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
| 2.4.1.292 | 3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | Campylobacter jejuni | - |
3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
| 2.4.1.293 | UDP-alpha-D-glucose + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | Campylobacter jejuni | - |
UDP + [GalNAc-alpha-(1->4)]2-[Glc-beta-(1->3)]-[GalNAc-alpha-(1->4)]2-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.289 | Campylobacter jejuni | - |
gene pglA, pgl gene cluster | - |
| 2.4.1.289 | Campylobacter jejuni NCTC 11168 | - |
gene pglA, pgl gene cluster | - |
| 2.4.1.290 | Campylobacter jejuni | - |
gene pglA | - |
| 2.4.1.290 | Campylobacter jejuni NCTC 11168 | - |
gene pglA | - |
| 2.4.1.291 | Campylobacter jejuni | - |
gene pglJ, pgl gene cluster | - |
| 2.4.1.291 | Campylobacter jejuni NCTC 11168 | - |
gene pglJ, pgl gene cluster | - |
| 2.4.1.292 | Campylobacter jejuni | - |
gene pglH, pgl gene cluster | - |
| 2.4.1.292 | Campylobacter jejuni NCTC 11168 | - |
gene pglH, pgl gene cluster | - |
| 2.4.1.293 | Campylobacter jejuni | - |
gene pglI, pgl gene cluster | - |
| 2.4.1.293 | Campylobacter jejuni NCTC 11168 | - |
gene pglI, pgl gene cluster | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.289 | recombinant His-tagged PglA from Escherichia coli by nickel affinity chromatography | Campylobacter jejuni |
| 2.4.1.290 | recombinant His-tagged PglA from Escherichia coli | Campylobacter jejuni |
| 2.4.1.291 | recombinant His-tagged PglJ from Escherichia coli by nickel affinity chromatography | Campylobacter jejuni |
| 2.4.1.292 | recombinant His-tagged PglH from Escherichia coli by nickel affinity chromatography | Campylobacter jejuni |
| 2.4.1.293 | recombinant His-tagged PglI from Escherichia coli by nickel affinity chromatography | Campylobacter jejuni |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.289 | dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
Campylobacter jejuni | dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
| 2.4.1.289 | dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
Campylobacter jejuni NCTC 11168 | dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol | - |
? | |
| 2.4.1.289 | additional information | in vitro, trisaccharide is prepared by using PglA and PglJ and then reacts singly with PglH | Campylobacter jejuni | ? | - |
? | |
| 2.4.1.289 | additional information | in vitro, trisaccharide is prepared by using PglA and PglJ and then reacts singly with PglH | Campylobacter jejuni NCTC 11168 | ? | - |
? | |
| 2.4.1.290 | UDP-N-acetyl-alpha-D-galactosamine + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
Campylobacter jejuni | UDP + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
| 2.4.1.290 | UDP-N-acetyl-alpha-D-galactosamine + N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
Campylobacter jejuni NCTC 11168 | UDP + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
| 2.4.1.291 | UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
Campylobacter jejuni | UDP + N-acetyl-D-galactosaminyl-alpha-(1->4)-N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
| 2.4.1.291 | UDP-N-acetyl-alpha-D-galactosamine + N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
Campylobacter jejuni NCTC 11168 | UDP + N-acetyl-D-galactosaminyl-alpha-(1->4)-N-acetyl-D-galactosaminyl-alpha-(1->3)-N,N'-diacetyl-alpha-D-bacillosaminyl-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
| 2.4.1.292 | 3 UDP-N-acetyl-alpha-D-galactosamine + GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
Campylobacter jejuni | 3 UDP + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
| 2.4.1.292 | additional information | in vitro, trisaccharide is prepared by using PglA and PglJ and then reacts singly with PglH | Campylobacter jejuni | ? | - |
? | |
| 2.4.1.292 | additional information | in vitro, trisaccharide is prepared by using PglA and PglJ and then reacts singly with PglH | Campylobacter jejuni NCTC 11168 | ? | - |
? | |
| 2.4.1.293 | UDP-alpha-D-glucose + [GalNAc-alpha-(1->4)]4-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
Campylobacter jejuni | UDP + [GalNAc-alpha-(1->4)]2-[Glc-beta-(1->3)]-[GalNAc-alpha-(1->4)]2-GalNAc-alpha-(1->3)-Bac2,4diNAc-diphospho-tritrans,heptacis-undecaprenol | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.289 | ? | x * 43000, recombinant His-tagged PglA, SDS-PAGE | Campylobacter jejuni |
| 2.4.1.290 | ? | x * 43000, recombinant His-tagged PglA, SDS-PAGE | Campylobacter jejuni |
| 2.4.1.291 | ? | x * 41000, recombinant His-tagged PglJ, SDS-PAGE | Campylobacter jejuni |
| 2.4.1.292 | ? | x * 41000, recombinant His-tagged PglH, SDS-PAGE | Campylobacter jejuni |
| 2.4.1.293 | ? | x * 36000, recombinant His-tagged Pgli, SDS-PAGE | Campylobacter jejuni |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 2.4.1.289 | PglA | - |
Campylobacter jejuni |
| 2.4.1.290 | PglA | - |
Campylobacter jejuni |
| 2.4.1.291 | PglJ | - |
Campylobacter jejuni |
| 2.4.1.292 | PglH | - |
Campylobacter jejuni |
| 2.4.1.293 | PglI | - |
Campylobacter jejuni |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.289 | 7 | 8 | assay at | Campylobacter jejuni |
| 2.4.1.290 | 8 | - |
assay at | Campylobacter jejuni |
| 2.4.1.291 | 7 | 8 | assay at | Campylobacter jejuni |
| 2.4.1.292 | 7 | 8 | assay at | Campylobacter jejuni |
| 2.4.1.293 | 7 | 8 | assay at | Campylobacter jejuni |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 2.4.1.289 | metabolism | PglA is part of a general N-linked glycosylation pathway, encoded by the pgl gene cluster, which culminates in the transfer of a heptasaccharide: GalNAc-alpha1,4-GalNAc-alpha1,4-(Glcbeta1,3)-GalNAc-alpha1,4-GalNAc-alpha1,4-GalNAc-alpha1,3-Bac, where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose), from a membrane-anchored undecaprenyldiphosphate-linked donor to the asparagine side chain of proteins at the Asn-X-Ser/Thr motif. The glycosyltransferases, PglA, PglH, PglI, and PglJ, responsible for the biosynthesis of the Und-PP-linked heptasaccharide. PglA adds the first GalNAc residue on to the isoprenoid-linked Bac carrier | Campylobacter jejuni |
| 2.4.1.290 | metabolism | a general N-linked glycosylation pathway, encoded by the pgl gene cluster, culminates in the transfer of a heptasaccharide: GalNAc-alpha1,4-GalNAc-alpha1,4-(Glcalpha1,3)-GalNAc-alpha1,4-GalNAc-alpha1,4-GalNAc-alpha1,3-Bac, where Bac is bacillosamine, i.e. 2,4-diacetamido-2,4,6-trideoxyglucose, from a membrane-anchored undecaprenylpyrophosphate (Und-PP)-linked donor to the asparagine side chain of proteins at the Asn-X-Ser/Thr motif. For biosynthesis of Und-PP-linked heptasaccharide, PglA and PglJ add the first two GalNAc residues on to the isoprenoid-linked Bac carrier, respectively. Elongation of the trisaccharide with PglH results in a hexasaccharide revealing the polymerase activity of PglH. The final branching glucose is then added by PglI, which prefers native lipids for optimal activity. Pathway overview | Campylobacter jejuni |
| 2.4.1.291 | metabolism | PglJ is part of a general N-linked glycosylation pathway, encoded by the pgl gene cluster, which culminates in the transfer of a heptasaccharide: GalNAc-alpha1,4-GalNAc-alpha1,4-(Glcbeta1,3)-GalNAc-alpha1,4-GalNAc-alpha1,4-GalNAc-alpha1,3-Bac, where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose), from a membrane-anchored undecaprenylpyrophosphate-linked donor to the asparagine side chain of proteins at the Asn-X-Ser/Thr motif. PglJ adds the second GalNAc residues on to the isoprenoid-linked Bac carrier. PglJ transfers a single sugar | Campylobacter jejuni |
| 2.4.1.292 | metabolism | PglH is part of a general N-linked glycosylation pathway, encoded by the pgl gene cluster, which culminates in the transfer of a heptasaccharide: GalNAc-alpha1,4-GalNAc-alpha1,4-(Glcbeta1,3)-GalNAc-alpha1,4-GalNAc-alpha1,4-GalNAc-alpha1,3-Bac, where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose), from a membrane-anchored undecaprenylpyrophosphate-linked donor to the asparagine side chain of proteins at the Asn-X-Ser/Thr motif. The glycosyltransferases, PglA, PglH, PglI, and PglJ, responsible for the biosynthesis of the Und-PP-linked heptasaccharide. Elongation of the trisaccharide with PglH results in a hexasaccharide revealing the polymerase activity of PglH | Campylobacter jejuni |
| 2.4.1.293 | metabolism | PglI is part of a general N-linked glycosylation pathway, encoded by the pgl gene cluster, which culminates in the transfer of a heptasaccharide: GalNAc-alpha1,4-GalNAc-alpha1,4-(Glcbeta1,3)-GalNAc-alpha1,4-GalNAc-alpha1,4-GalNAc-alpha1,3-Bac, where Bac is bacillosamine (2,4-diacetamido-2,4,6-trideoxyglucose), from a membrane-anchored undecaprenyldiphosphate-linked donor to the asparagine side chain of proteins at the Asn-X-Ser/Thr motif. The glycosyltransferases, PglA, PglH, PglI, and PglJ, responsible for the biosynthesis of the undecaprenyl diphoshate-linked heptasaccharide. The final branching glucose is added by PglI, and the flippase, WlaB, then flips the completed lipid-linked heptasaccharide into the periplasm | Campylobacter jejuni |
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