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Literature summary extracted from

  • Pecsi, I.; Hirmondo, R.; Brown, A.C.; Lopata, A.; Parish, T.; Vertessy, B.G.; Toth, J.
    The dUTPase enzyme is essential in Mycobacterium smegmatis (2012), PLoS ONE, 7, e37461.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.6.1.23 medicine dUTPase is a target for antitubercular drugs Mycobacterium tuberculosis
3.6.1.23 medicine dUTPase is a target for antitubercular drugs Mycolicibacterium smegmatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expressed in Escherichia coli BL21(DE3) (pLysS) cells Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.6.1.23 additional information the DELTA-loop deletion mutant is created by deletion of the five (Ala133-Ser137) loop-specific amino acids and shows reduced kcat and increased Km values for dUTP Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.0009
-
 dUTP wild type enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C Mycobacterium tuberculosis
3.6.1.23 0.0011
-
 dUTP DELTA-loop mutant enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Mycobacterium tuberculosis
-
 dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O Mycolicibacterium smegmatis
-
 dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
 dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O Mycobacterium tuberculosis H37Rv
-
 dUMP + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Mycobacterium tuberculosis P9WNS5
-
-
3.6.1.23 Mycobacterium tuberculosis H37Rv P9WNS5
-
-
3.6.1.23 Mycolicibacterium smegmatis A0QW08
-
-
3.6.1.23 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 A0QW08
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 Ni-NTA column chromatography Mycobacterium tuberculosis
3.6.1.23 Ni-NTA column chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUTP + H2O
-
 Mycobacterium tuberculosis dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Mycolicibacterium smegmatis dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 dUMP + diphosphate
-
 ?
3.6.1.23 dUTP + H2O
-
 Mycobacterium tuberculosis H37Rv dUMP + diphosphate
-
 ?
3.6.1.23 additional information the enzyme cannot hydrolyze alpha-beta-imido-dUTP Mycobacterium tuberculosis ?
-
 ?
3.6.1.23 additional information the enzyme cannot hydrolyze alpha-beta-imido-dUTP Mycolicibacterium smegmatis ?
-
 ?
3.6.1.23 additional information the enzyme cannot hydrolyze alpha-beta-imido-dUTP Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 ?
-
 ?
3.6.1.23 additional information the enzyme cannot hydrolyze alpha-beta-imido-dUTP Mycobacterium tuberculosis H37Rv ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.6.1.23 deoxyuridine 5’-triphosphate nucleotidohydrolase
-
 Mycobacterium tuberculosis
3.6.1.23 deoxyuridine 5’-triphosphate nucleotidohydrolase
-
 Mycolicibacterium smegmatis
3.6.1.23 DUT
-
 Mycobacterium tuberculosis
3.6.1.23 DUT
-
 Mycolicibacterium smegmatis
3.6.1.23 dUTPase
-
 Mycobacterium tuberculosis
3.6.1.23 dUTPase
-
 Mycolicibacterium smegmatis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 0.88
-
 dUTP DELTA-loop mutant enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C Mycobacterium tuberculosis
3.6.1.23 1.22
-
 dUTP wild type enzyme, in 1 mM HEPES pH 7.5, 100 mM KCl, and 5 mM MgCl2, at 20°C Mycobacterium tuberculosis

General Information

EC Number General Information Comment Organism
3.6.1.23 malfunction dUTPase knock-out results in lethality Mycolicibacterium smegmatis