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Literature summary extracted from

  • Rose, A.; Glassgen, W.E.; Hopp, W.; Seitz, H.U.
    Purification and characterization of glycosyltransferases involved in anthocyanin biosynthesis in cell-suspension cultures of Daucus carota L. (1996), Planta, 198, 397-403.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.50 0.1
-
 cyanidin 3-O-beta-D-galactoside pH 7.5, 32°C, crude enzyme Daucus carota
2.4.2.50 0.2
-
 UDP-alpha-D-xylose pH 7.5, 32°C, crude enzyme Daucus carota

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.294 soluble
-
 Daucus carota
-
-
2.4.2.50 soluble
-
 Daucus carota
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.294 additional information divalent cations have no influence on the enzyme activity Daucus carota
2.4.2.50 additional information divalent cations have no influence on the enzyme activity Daucus carota

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.294 UDP-alpha-D-galactose + cyanidin Daucus carota
-
 UDP + cyanidin 3-O-beta-D-galactoside
-
 ?
2.4.2.50 UDP-alpha-D-xylose + cyanidin 3-O-beta-D-galactoside Daucus carota
-
 UDP + cyanidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-galactoside)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.294 Daucus carota
-
 Afghan cultivar carrot
-
2.4.1.294 Daucus carota
-
 Afghan cultivar
-
2.4.2.50 Daucus carota
-
 Afghan cultivar carrot
-
2.4.2.50 Daucus carota
-
 Afghan cultivar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.294 native enzyme 526fold by ammonium sulfate fractionation and ultracentrifugation, the enzyme is then separated from the UDP-xylose:cyanidin 3-galactoside xylosyltransferase, CGXT, by anion exchange and affinity chromatography, followed by gel filtation Daucus carota
2.4.2.50 partially, by ammonium sulfate fractionation and ultracentrifugation, the enzyme is then separated from the UDP-galactose:cyanidin galactosyltransferase, CGT, by anion exchange and affinity chromatography. CGXT activity is lost after another step of gel filtration Daucus carota

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.294 cell suspension culture
-
 Daucus carota
-
2.4.2.50 cell suspension culture
-
 Daucus carota
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.294 UDP-alpha-D-galactose + cyanidin
-
 Daucus carota UDP + cyanidin 3-O-beta-D-galactoside
-
 ?
2.4.2.50 UDP-alpha-D-xylose + cyanidin 3-O-beta-D-galactoside
-
 Daucus carota UDP + cyanidin 3-O-(beta-D-xylosyl-(1->2)-beta-D-galactoside)
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.294 CGT
-
 Daucus carota
2.4.1.294 UDP-galactose:cyanidin galactosyltransferase
-
 Daucus carota
2.4.2.50 CGXT
-
 Daucus carota
2.4.2.50 UDP-xylose: cyanidin 3-galactoside xylosyltransferase
-
 Daucus carota

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.294 23
-
-
 Daucus carota
2.4.2.50 32
-
-
 Daucus carota

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.294 6.8
-
-
 Daucus carota
2.4.2.50 6.9
-
-
 Daucus carota