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Literature summary extracted from

  • Neelakandan, A.K.; Nguyen, H.T.; Kumar, R.; Tran, L.S.; Guttikonda, S.K.; Quach, T.N.; Aldrich, D.L.; Nes, W.D.; Nguyen, H.T.
    Molecular characterization and functional analysis of Glycine max sterol methyl transferase 2 genes involved in plant membrane sterol biosynthesis (2010), Plant Mol. Biol., 74, 503-518.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.143 genes SMT2-1 and SMT2-2, generation of a beta-conglycinin-AtHMGR1:glycinin-GmSMT1 construct and a glycinin-GmSMT1-pZY101-Asc construct, and of a a three gene casette vector beta-conglycinin-AtHMGR1:glycinin-GmSMT1:beta-phaseolin-GmSMT2-2, overview. cis-Acting elements occur in SMT2 upstream sequences, motifs responsible for basal and regulated expression, overview. Expression of SMT2 in Arabidopsis thaliana ecotype Columbia plants, expression of genes SMT2-1 and SMT2-2 complements the SMT deficient cvp1 mutant Arabidopsis plants. The transgenic plants harboring the SMT2-1 promoter show enzyme-GUS expression predominantly in the cotyledons and the base of the hypocotyls in seedlings, and the tips of young leaves. Transgenic plants with the SMT2-2 promoter had a similar expression pattern, but with strong enzyme-Gus expression throughout the leaves and stem, specifically in the vascular tissue, in both etiolated and light-grown plants Glycine max

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.143 additional information generation of transgenic Arabidopsis thaliana plants expressing gene SMT-2 from Glycine max under control of the CaMV S35 promoter, functional complementation of SMT deficient cvp1 mutant Arabidopsis plants. Role of SMT2 in Arabidopsis seed sterol accumulation, changes in the total sterol content in transgenic seeds, phenotypes, overview Glycine max

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.142 Arabidopsis thaliana
-
-
-
2.1.1.142 Glycine max
-
-
-
2.1.1.143 Glycine max D2D5G3 SMT2-1
-
2.1.1.143 Glycine max D2D5G4 SMT2-2
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.143 seedling
-
 Glycine max
-

Synonyms

EC Number Synonyms Comment Organism
2.1.1.142 SMT2-1
-
 Glycine max
2.1.1.142 sterol C24-methyltransferase
-
 Glycine max
2.1.1.142 sterol C24-methyltransferase
-
 Arabidopsis thaliana
2.1.1.143 SMT2
-
 Glycine max
2.1.1.143 sterol methyl transferase 2
-
 Glycine max

Expression

EC Number Organism Comment Expression
2.1.1.142 Glycine max transcriptional regulation of soybean SMT2 genes is influenced by environmental conditions to some extent: analysis of transcript abundance by qRT-PCR reveals that both the genes SMT2-1 and SMT2-2 are down-regulated after dehydration and markedly induced under cold conditions. ABA treatment elicits an increase in transcript levels for both the genes. A reduction in expression is associated with the salinity treatment for both the genes additional information
2.1.1.143 Glycine max environmental cues, including dehydration, cold, and abscisic acid induce differential changes in transcript levels of the SMT2 during soybean seedling growth additional information

General Information

EC Number General Information Comment Organism
2.1.1.142 malfunction the SMT2 genes of soybean (SMT2-1 and SMT2-2) are shown to complement the SMT deficient cvp1 mutant Arabidopsis plants Arabidopsis thaliana
2.1.1.142 malfunction transgenic Arabidopsis seeds expressing GmSMT1 or GmSMT2 show modifications in the ratio of 24-methyl to 24-ethyl sterol in the direction of sitosterol formation Glycine max
2.1.1.143 metabolism SMT2 is a rate-limiting enzyme in channeling the carbon flux downstream towards C-24 alkyl sterol synthesis Glycine max
2.1.1.143 physiological function SMT-1 and SMT-2 play a role in regulation of 24-alkyl sterol-controlled plant physiology. The reaction catalyzed by the SMT2 enzyme diverts carbon flux towards the C-24 ethyl sterols, which represents the final segment of phytosterol biosynthesis. SMT-2 plays a role in seed sterol accumulation Glycine max