EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.151 | DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic tree, expression of C-terminally His-tagged BPS in Escherichia coli | Garcinia mangostana |
2.3.1.220 | DNA and amin acid sequence determination and analysis, sequence comparison and phylogenetic tree | Garcinia mangostana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.3.1.151 | A257G | site-directed mutagenesis, the mutant shows an altered product pattern compared to the wild-type enzyme | Garcinia mangostana |
2.3.1.151 | G339S | site-directed mutagenesis, the mutant shows an altered product pattern compared to the wild-type enzyme | Garcinia mangostana |
2.3.1.151 | G339V | site-directed mutagenesis, the mutant shows an altered product pattern compared to the wild-type enzyme | Garcinia mangostana |
2.3.1.151 | T133L | site-directed mutagenesis, the mutant shows an altered product pattern compared to the wild-type enzyme | Garcinia mangostana |
2.3.1.220 | A257G | site-directed mutagenesis, altered substrate entrance in the active site structure compared to the wild-type enzyme. The mutant A257G gives similar enzyme products as the wild type with both benzoyl-CoA and 4-coumaroyl-CoA | Garcinia mangostana |
2.3.1.220 | G339S | site-directed mutagenesis, altered substrate entrance in the active site structure compared to the wild-type enzyme. The mutant G339S yields high amounts of both benzophenone and triketide lactone type with benzoyl-CoA but shows no activity for 4-coumaroyl-CoA | Garcinia mangostana |
2.3.1.220 | G339V | site-directed mutagenesis, altered substrate entrance in the active site structure compared to the wild-type enzyme. The mutant shows no activity with either benzoyl-CoA or 4-coumaroyl-CoA | Garcinia mangostana |
2.3.1.220 | additional information | homology model of wild-type GmBPS and GmBPS mutants A257G, T133L, G339V, and G339S, active-site architectures by surface models and substrate entrances, overview | Garcinia mangostana |
2.3.1.220 | T133L | site-directed mutagenesis, altered substrate entrance in the active site structure compared to the wild-type enzyme. With benzoyl-CoA as a starter, the T133L mutant yields triketide lactone as the major enzymatic product | Garcinia mangostana |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.151 | 0.00963 | - |
benzoyl-CoA | pH 7.0, 30°C, recombinant His-tagged BPS | Garcinia mangostana | |
2.3.1.151 | 0.01638 | - |
malonyl-CoA | pH 7.0, 30°C, recombinant His-tagged BPS | Garcinia mangostana | |
2.3.1.220 | 0.00965 | - |
benzoyl-CoA | pH 7.0, 30°C | Garcinia mangostana | |
2.3.1.220 | 0.0164 | - |
malonyl-CoA | pH 7.0, 30°C | Garcinia mangostana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.151 | 44000 | - |
2 * 44000, His-tagged enzyme, SDS-PAGE; 2 * 45618, His-tagged enzyme, sequence calculation | Garcinia mangostana |
2.3.1.151 | 45618 | - |
2 * 44000, His-tagged enzyme, SDS-PAGE; 2 * 45618, His-tagged enzyme, sequence calculation | Garcinia mangostana |
2.3.1.151 | 88000 | - |
His-tagged enzyme, gel filtration | Garcinia mangostana |
2.3.1.220 | 42700 | - |
xx * 42700, about, sequence calculation | Garcinia mangostana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.220 | 3 malonyl-CoA + benzoyl-CoA | Garcinia mangostana | - |
4 CoA + 2,4,6-trihydroxybenzophenone + 3 CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.151 | Garcinia mangostana | L7NCQ3 | - |
- |
2.3.1.220 | Garcinia mangostana | L7NCQ3 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.151 | recombinant C-terminally His-tagged BPS from Escherichia coli | Garcinia mangostana |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.151 | pericarp | expression of GmBPS in fruit pericarp from an early stage | Garcinia mangostana | - |
2.3.1.220 | fruit | - |
Garcinia mangostana | - |
2.3.1.220 | pericarp | - |
Garcinia mangostana | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.151 | 2 malonyl-CoA + 4-coumaroyl-CoA | - |
Garcinia mangostana | 3 CoA + 4-hydroxy-6-[(E)-2-(4-hydroxyphenyl)ethenyl]-2H-pyran-2-one + 2 CO2 | - |
? | |
2.3.1.151 | 3 malonyl-CoA + acetyl-CoA | - |
Garcinia mangostana | 4 CoA + 4-hydroxy-6-methyl-2H-pyran-2-one + 3 CO2 | - |
? | |
2.3.1.151 | 3 malonyl-CoA + benzoyl-CoA | - |
Garcinia mangostana | 4 CoA + 2,4,6-trihydroxybenzophenone + 4-hydroxy-6-(2-oxo-2-phenylethyl)-2H-pyran-2-one + 4-hydroxy-6-phenyl-2H-pyran-2-one + 3 CO2 | the recombinant enzyme produces 2,4,6-trihydroxybenzophenone as the predominant product with benzoyl CoA as substrate but also lactones of tetraketide and triketide type | ? | |
2.3.1.151 | 3 malonyl-CoA + cinnamoyl-CoA | - |
Garcinia mangostana | 4 CoA + 4-hydroxy-6-[(E)-2-phenylethenyl]-2H-pyran-2-one + 3 CO2 | - |
? | |
2.3.1.151 | 3 malonyl-CoA + hexanoyl-CoA | - |
Garcinia mangostana | 4 CoA + 4-hydroxy-6-pentyl-2H-pyran-2-one + 5-hexanoyl-4-hydroxy-6-methyl-2H-pyran-2-one + 3 CO2 | - |
? | |
2.3.1.151 | 3 malonyl-CoA + phenylacetyl-CoA | - |
Garcinia mangostana | 4 CoA + 4-hydroxy-6-(2-oxo-3-phenylpropyl)-2H-pyran-2-one + 6-benzyl-4-hydroxy-2H-pyran-2-one + 2-phenyl-1-(2,4,6-trihydroxyphenyl)ethanone + 3 CO2 | - |
? | |
2.3.1.151 | 3 malonyl-CoA + salicyl-CoA | - |
Garcinia mangostana | 4 CoA + 4-hydroxy-2H-chromen-2-one + 3 CO2 | - |
? | |
2.3.1.151 | 4 malonyl-CoA | - |
Garcinia mangostana | 4 CoA + 4-hydroxy-6-methyl-2H-pyran-2-one + 3 CO2 | - |
? | |
2.3.1.151 | additional information | with acetyl CoA or malonyl CoA as a starter substrate, the enzyme only produces a triacetic lactone of triketide lactone type, with phenylacetyl-Coa also lactones of tetraketide and triketide type, and with hexanoyl-CoA phloroglucinol and triketide type lactones, substrate specificity, overview | Garcinia mangostana | ? | - |
? | |
2.3.1.220 | 3 malonyl-CoA + benzoyl-CoA | - |
Garcinia mangostana | 4 CoA + 2,4,6-trihydroxybenzophenone + 3 CO2 | - |
? | |
2.3.1.220 | 3 malonyl-CoA + benzoyl-CoA | a minor amount of tetraketide lactone and triketide lactone are also obtained | Garcinia mangostana | 4 CoA + 2,4,6-trihydroxybenzophenone + 3 CO2 | - |
? | |
2.3.1.220 | additional information | the recombinant enzyme produces 2,4,6-trihydroxybenzophenone as the predominant product with benzoyl CoA as substrate. It also accepts other substrates, such as other plant PKSs, and used 1-3 molecules of malonyl CoA to form various phloroglucinol-type and polyketide lactone-type compounds | Garcinia mangostana | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.151 | dimer | 2 * 44000, His-tagged enzyme, SDS-PAGE; 2 * 45618, His-tagged enzyme, sequence calculation | Garcinia mangostana |
2.3.1.220 | ? | xx * 42700, about, sequence calculation | Garcinia mangostana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.151 | BPS | - |
Garcinia mangostana |
2.3.1.220 | benzophenone synthase | - |
Garcinia mangostana |
2.3.1.220 | BPS | - |
Garcinia mangostana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.3.1.151 | 30 | - |
recombinant His-tagged BPS | Garcinia mangostana |
2.3.1.220 | 30 | - |
- |
Garcinia mangostana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.151 | 0.049 | - |
benzoyl-CoA | pH 7.0, 30°C, recombinant His-tagged BPS | Garcinia mangostana | |
2.3.1.151 | 0.058 | - |
malonyl-CoA | pH 7.0, 30°C, recombinant His-tagged BPS | Garcinia mangostana | |
2.3.1.220 | 0.0495 | - |
benzoyl-CoA | pH 7.0, 30°C | Garcinia mangostana | |
2.3.1.220 | 0.0582 | - |
malonyl-CoA | pH 7.0, 30°C | Garcinia mangostana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.3.1.151 | 6.5 | 7 | recombinant His-tagged BPS | Garcinia mangostana |
2.3.1.220 | 6.5 | 7 | - |
Garcinia mangostana |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.3.1.151 | Garcinia mangostana | sequence calculation | - |
5.97 |
2.3.1.220 | Garcinia mangostana | sequence calculation | - |
5.97 |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.151 | evolution | Garcinia mangostana BPS is an enzyme in the family of type III polyketide synthases and shows an amino acid sequence identity of 77-78% with other plant BPSs belonging to the same family (Clusiaceae). The three BPSs from the xanthone-rich Clusiaceae plants, Garcinia mangostana, Hypericum androsaemum, and Hypericum perforatum, suggest a close evolutionary relationship among these enzymes | Garcinia mangostana |
2.3.1.151 | additional information | the catalytic triad is formed by Cys164, His303, and Asn336 | Garcinia mangostana |
2.3.1.220 | evolution | phylogenetic tree | Garcinia mangostana |
2.3.1.220 | metabolism | the enzyme catalyzes the formation of an intermediate, 2,4,6-trihydroxybenzophenone, in the biosynthetic pathway of alpha-mangostin | Garcinia mangostana |
2.3.1.220 | additional information | the catalytic triad is formed by Cys164, His303, and Asn336 | Garcinia mangostana |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.220 | 3.55 | - |
malonyl-CoA | pH 7.0, 30°C | Garcinia mangostana | |
2.3.1.220 | 5.14 | - |
benzoyl-CoA | pH 7.0, 30°C | Garcinia mangostana |