EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.46 | reduced glutathione | required for activity | Claviceps purpurea |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.46 | expressed in Escherichia coli BXL1 Blue MRF cells | Claviceps purpurea |
1.5.1.46 | expressed in Escherichia coli strain XL1 Blue MRF | Claviceps purpurea |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.46 | 30000 | - |
1 * 30000, His-tagged enzyme, SDS-PAGE | Claviceps purpurea |
1.5.1.46 | 30000 | - |
x * 30000, His-tagged enzyme, SDS-PAGE | Claviceps purpurea |
1.5.1.46 | 31900 | - |
1 * 31900, native enzyme, calculated from amino acid sequence | Claviceps purpurea |
1.5.1.46 | 31900 | - |
x * 31900, calculated from amino acid sequence | Claviceps purpurea |
1.5.1.46 | 33300 | - |
1 * 33300, His-tagged enzyme, calculated from amino acid sequence | Claviceps purpurea |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.44 | Aspergillus fumigatus | - |
- |
- |
1.5.1.46 | Claviceps purpurea | - |
- |
- |
1.5.1.46 | Claviceps purpurea | P0CT21 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.46 | Ni-NTA agarose column chromatography | Claviceps purpurea |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.44 | chanoclavine-I aldehyde + NADH + H+ | this reaction occurs in the presence of the old yellow enzyme FgaOx3. Together with GSH and NADPH, the enzyme also catalyses the conversion of chanoclavine-I aldehyde to agroclavine in the absence of the old yellow enzyme FgaOx3 | Aspergillus fumigatus | festuclavine + NAD+ | - |
? | |
1.5.1.46 | chanoclavine-I aldehyde + NADH + H+ | 54% conversion compared to NADPH, in the presence of reduced glutathione | Claviceps purpurea | agroclavine + NAD+ | - |
? | |
1.5.1.46 | chanoclavine-I aldehyde + NADH + H+ | the reaction occurs only in the presence of reduced glutathione (conversion rate of 54%) | Claviceps purpurea | agroclavine + NAD+ | - |
? | |
1.5.1.46 | chanoclavine-I aldehyde + NADPH + H+ | the reaction occurs only in the presence of reduced glutathione or beta-mercaptoethanol or dithiothreitol | Claviceps purpurea | agroclavine + NADP+ | - |
? | |
1.5.1.46 | chanoclavine-I aldehyde + NADPH + H+ | the reaction occurs only in the presence of the thiolation agents reduced glutathione (conversion rate of 59%) or 2-mercaptoethanol (conversion rate of 99%) and dithiothreitol (conversion rate of 67%). No product formation is detected in the incubation mixture with L-ascorbic acid instead of GSH | Claviceps purpurea | agroclavine + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.5.1.46 | ? | x * 30000, His-tagged enzyme, SDS-PAGE | Claviceps purpurea |
1.5.1.46 | ? | x * 31900, calculated from amino acid sequence | Claviceps purpurea |
1.5.1.46 | monomer | 1 * 30000, His-tagged enzyme, SDS-PAGE | Claviceps purpurea |
1.5.1.46 | monomer | 1 * 31900, native enzyme, calculated from amino acid sequence | Claviceps purpurea |
1.5.1.46 | monomer | 1 * 33300, His-tagged enzyme, calculated from amino acid sequence | Claviceps purpurea |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.1.44 | festuclavine synthase | - |
Aspergillus fumigatus |
1.5.1.44 | FgaFS | - |
Aspergillus fumigatus |
1.5.1.46 | agroclavine synthase | - |
Claviceps purpurea |
1.5.1.46 | EasG | - |
Claviceps purpurea |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.46 | NADH | - |
Claviceps purpurea | |
1.5.1.46 | NADPH | - |
Claviceps purpurea |