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Literature summary extracted from

  • Menezes, S.; Gaston, K.W.; Krivos, K.L.; Apolinario, E.E.; Reich, N.O.; Sowers, K.R.; Limbach, P.A.; Perona, J.J.
    Formation of m2G6 in Methanocaldococcus jannaschii tRNA catalyzed by the novel methyltransferase Trm14 (2011), Nucleic Acids Res., 39, 7641-7655.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.256 gene MJ0438 or trm14, DNA and amino acid sequence determination, analysis and comparisons, phylogenetic analysis Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.256 additional information
-
 additional information single-turnover, kinetic analysis, overview Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.1.1.256 Mg2+ required Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.256 S-adenosyl-L-methionine + guanine6 in tRNACys Methanocaldococcus jannaschii Trm14 generates m2G at position 6 in tRNACys S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
 ?
2.1.1.256 S-adenosyl-L-methionine + guanine6 in tRNACys Methanocaldococcus jannaschii JAL-1 Trm14 generates m2G at position 6 in tRNACys S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.256 Methanocaldococcus jannaschii Q57880 DSM 2661, gene MJ0438 or trm14
-
2.1.1.256 Methanocaldococcus jannaschii JAL-1 Q57880 DSM 2661, gene MJ0438 or trm14
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.256 S-adenosyl-L-methionine + guanine6 in tRNACys Trm14 generates m2G at position 6 in tRNACys Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
 ?
2.1.1.256 S-adenosyl-L-methionine + guanine6 in tRNACys wild-type or G6A/C67U tRNACys transcripts as substrates, Trm14 generates m2G at position 6 in tRNACys, i.e. tRNACys m2G6-forming activity Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
 ?
2.1.1.256 S-adenosyl-L-methionine + guanine6 in tRNACys Trm14 generates m2G at position 6 in tRNACys Methanocaldococcus jannaschii JAL-1 S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
 ?
2.1.1.256 S-adenosyl-L-methionine + guanine6 in tRNACys wild-type or G6A/C67U tRNACys transcripts as substrates, Trm14 generates m2G at position 6 in tRNACys, i.e. tRNACys m2G6-forming activity Methanocaldococcus jannaschii JAL-1 S-adenosyl-L-homocysteine + N2-methylguanine6 in tRNACys
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.256 More the 381 amino acid Trm14 protein possesses a canonical RNA recognition THUMP domain at the N-terminus, followed by a gamma-class Rossmann fold amino-methyltransferase catalytic domain featuring the signature NPPY active site motif Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
2.1.1.256 m2G6 tRNA methylase
-
 Methanocaldococcus jannaschii
2.1.1.256 methyltransferase Trm14
-
 Methanocaldococcus jannaschii
2.1.1.256 Trm14
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.256 37
-
 assay at Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.256 7.3
-
 assay at Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
2.1.1.256 evolution Trm14 is associated with cluster of orthologous groups, COG, 0116, and most closely resembles the m2G10 tRNA methylase Trm11. Phylogenetic analysis reveals a canonical archaeal/bacterial evolutionary separation with 20-30% sequence identities between the two branches, but it is likely that the detailed functions of COG 0116 enzymes differ between the archaeal and bacterial domains. Phylogenetic distribution of Trm14-like proteins, overview Methanocaldococcus jannaschii
2.1.1.256 physiological function tRNA m2G6 formation may play a role in stabilizing the structure of the RNA and function synergistically with these core-region modifications to stabilize the molecule against thermal stress. Also, Trm14-catalyzed m2G6 formation in tRNACys may play a role in modulating the aminoacylation efficiency of phosphoseryl-tRNA synthetase, i.e. SepRS. Control of SepRS (or CysRS) activity by SAM-dependent methylation of tRNACys might provide the methanogen cell with a regulatory mechanism by which protein synthesis rates can respond to environmental sulfur levels or to concentrations of sulfur metabolites Methanocaldococcus jannaschii