Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Sun, M.; Zhu, G.; Qin, Z.; Wu, C.; Lv, M.; Liao, S.; Qi, N.; Xie, M.; Cai, J.
    Functional characterizations of malonyl-CoA:acyl carrier protein transacylase (MCAT) in Eimeria tenella (2012), Mol. Biochem. Parasitol., 184, 20-28.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.39 expressed in Escherichia coli Eimeria tenella

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.39 corytuberine
-
 Eimeria tenella

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.39 0.0228
-
 malonyl-CoA pH 6.8, 28°C Eimeria tenella

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.39 apicoplast
-
 Eimeria tenella 20011
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.39 38800
-
 calculated from cDNA Eimeria tenella

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.39 Eimeria tenella
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.3.1.39 proteolytic modification computer analysis predicts that EtMCAT protein contains a 20 aa N-terminal signal peptide plus 37 aa transit peptide for targeting and translocating into the apicoplast Eimeria tenella

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.39 malonyl-CoA + acyl-carrier protein alpha-ketoglutarate dehydrogenase (KDH)-coupled assay system is used: KDH-dependent consumption of coenzyme A (CoA) generated by MCAT is accompanied by a reduction of nicotinamide adenine dinucleotide (NAD+) to NADH Eimeria tenella CoA + malonyl-[acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.39 EtMCAT
-
 Eimeria tenella
2.3.1.39 FabD
-
 Eimeria tenella
2.3.1.39 malonyl-CoA:acyl carrier protein transacylase
-
 Eimeria tenella

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.39 28
-
 assay at Eimeria tenella

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.39 6.8
-
 assay at Eimeria tenella

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.39 0.01647
-
 pH 6.8, 28°C Eimeria tenella corytuberine

General Information

EC Number General Information Comment Organism
2.3.1.39 malfunction functional replacement of the fabD gene with amber mutation of Escherichia coli temperature-sensitive LA2-89 strain by Eimeria tenella EtMCAT demonstrates that EcFabD and EtMCAT perform the same biochemical function Eimeria tenella