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Literature summary extracted from

  • Chittori, S.; Savithri, H.S.; Murthy, M.R.
    Crystal structure of Salmonella typhimurium 2-methylcitrate synthase: Insights on domain movement and substrate specificity (2011), J. Struct. Biol., 174, 58-68.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.5 ATP
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.3.5 pyruvate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.5 0.013
-
 oxaloacetate using acetyl-CoA as cosubstrate, in 50 mM HEPES pH 8.0, at 30°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.3.5 0.015
-
 oxaloacetate using propanoyl-CoA as cosubstrate, in 50 mM HEPES pH 8.0, at 30°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.3.5 0.045
-
 propanoyl-CoA in 50 mM HEPES pH 8.0, at 30°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.3.5 0.265
-
 acetyl-CoA in 50 mM HEPES pH 8.0, at 30°C Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.5 Salmonella enterica subsp. enterica serovar Typhimurium Q56063
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.5 acetyl-CoA + H2O + oxaloacetate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.3.3.5 butyryl-CoA + H2O + oxaloacetate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.3.3.5 propanoyl-CoA + H2O + oxaloacetate propanoyl-CoA is the most efficient substrate Salmonella enterica subsp. enterica serovar Typhimurium 2-methylcitrate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.5 decamer
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Synonyms

EC Number Synonyms Comment Organism
2.3.3.5 2-MCS
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.3.3.5 PrpC
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.5 52 60 the enzyme has a melting temperature of 52°C. Tm increases to 60°C when the enzyme is preincubated with 0.1 mM oxaloacetate Salmonella enterica subsp. enterica serovar Typhimurium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.3.5 1.87
-
 acetyl-CoA in 50 mM HEPES pH 8.0, at 30°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.3.5 8.25
-
 propanoyl-CoA in 50 mM HEPES pH 8.0, at 30°C Salmonella enterica subsp. enterica serovar Typhimurium

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.3.3.5 7.05
-
 acetyl-CoA in 50 mM HEPES pH 8.0, at 30°C Salmonella enterica subsp. enterica serovar Typhimurium
2.3.3.5 183
-
 propanoyl-CoA in 50 mM HEPES pH 8.0, at 30°C Salmonella enterica subsp. enterica serovar Typhimurium