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Literature summary extracted from

  • Ogata, J.; Sakamoto, T.; Yamaguchi, M.; Kawanobu, S.; Yoshitama, K.
    Isolation and characterization of anthocyanin 5-O-glucosyltransferase from flowers of Dahlia variabilis (2001), J. Plant Physiol., 158, 709-714.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.298 2-mercaptoethanol
-
 Dahlia pinnata
2.4.1.298 dithioerythritol
-
 Dahlia pinnata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.298 Al3+
-
 Dahlia pinnata
2.4.1.298 Cu2+
-
 Dahlia pinnata
2.4.1.298 Fe3+
-
 Dahlia pinnata
2.4.1.298 Hg2+
-
 Dahlia pinnata
2.4.1.298 iodoacetic acid slight inhibition Dahlia pinnata
2.4.1.298 Mn2+
-
 Dahlia pinnata
2.4.1.298 additional information poor inhibition by EDTA and ascorbic acid Dahlia pinnata
2.4.1.298 NEM
-
 Dahlia pinnata
2.4.1.298 PCMB
-
 Dahlia pinnata
2.4.1.298 Zn2+
-
 Dahlia pinnata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.298 Ca2+ slight activation Dahlia pinnata
2.4.1.298 Mg2+ slight activation Dahlia pinnata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.298 53000
-
 gel filtration Dahlia pinnata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.298 UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside Dahlia pinnata
-
 UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside Dahlia pinnata Sou-Une
-
 UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside Dahlia pinnata
-
 UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside Dahlia pinnata Sou-Une
-
 UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucoside Dahlia pinnata
-
 UDP + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucosyl-5-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside Dahlia pinnata
-
 UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside Dahlia pinnata Sou-Une
-
 UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.298 Dahlia pinnata
-
-
-
2.4.1.298 Dahlia pinnata Sou-Une
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.298 native enzyme 88fold by ammonium sulfate fractionation, anion exchange chromatography, gel filtration, and another different step of anion exchange chromatography Dahlia pinnata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.298 flower
-
 Dahlia pinnata
-
2.4.1.298 flower bud
-
 Dahlia pinnata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.298 0.18
-
 purified enzyme, pH 8.0, 35°C Dahlia pinnata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.298 additional information neither ADP-glucose nor UDP-galactose could serve as glycosyl donor Dahlia pinnata ?
-
 ?
2.4.1.298 additional information neither ADP-glucose nor UDP-galactose could serve as glycosyl donor Dahlia pinnata Sou-Une ?
-
 ?
2.4.1.298 UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
 Dahlia pinnata UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + cyanidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
 Dahlia pinnata Sou-Une UDP + cyanidin 3-O-beta-D-glucosyl-5-O-(6-O-malonyl)-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
 Dahlia pinnata UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + cyanidin 3-O-beta-D-glucoside
-
 Dahlia pinnata Sou-Une UDP + cyanidin 3,5-di-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside low activity Dahlia pinnata UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + delphinidin 3-O-beta-D-glucoside low activity Dahlia pinnata Sou-Une UDP + delphinidin 3,5-di-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucoside
-
 Dahlia pinnata UDP + pelargonidin 3-O-(6-O-malonyl)-beta-D-glucosyl-5-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
-
 Dahlia pinnata UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
 ?
2.4.1.298 UDP-alpha-D-glucose + pelargonidin 3-O-beta-D-glucoside
-
 Dahlia pinnata Sou-Une UDP + pelargonidin 3,5-di-O-beta-D-glucoside
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.298 5GT
-
 Dahlia pinnata
2.4.1.298 anthocyanin 5-O-glucosyltransferase
-
 Dahlia pinnata
2.4.1.298 UDP-glucose:anthocyanin 5-O-glucosyltransferase
-
 Dahlia pinnata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.298 35
-
 assay at Dahlia pinnata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.298 8
-
-
 Dahlia pinnata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.298 6.5 8.7 50% of maximal activity at pH 6.5 and pH 8.7 Dahlia pinnata

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.298 Dahlia pinnata
-
-
 4.2