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Literature summary extracted from

  • Schuman, B.; Persson, M.; Landry, R.C.; Polakowski, R.; Weadge, J.T.; Seto, N.O.; Borisova, S.N.; Palcic, M.M.; Evans, S.V.
    Cysteine-to-serine mutants dramatically reorder the active site of human ABO(H) blood group B glycosyltransferase without affecting activity: structural insights into cooperative substrate binding (2010), J. Mol. Biol., 402, 399-411.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.37 expressed in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.37 C80S/C196S both the double and triple mutants show differing levels of disorder depending on their liganded state, with the level of disorder increasing when substrates are bound.The double mutant shows disorder over residues 177-180 in the unliganded and H-antigen bound forms, with disorder increasing to residues 176-185 for the UDP-, UDP + H-, and UDP-alpha-D-galactose + 3-deoxy-Gal inactive acceptor analog-bound structures. The double mutant has a reduction in Km for both donor and acceptor substrates. Mutation shows little effect over kcat Homo sapiens
2.4.1.37 C80S/C196S/C209S both the double and triple mutants show differing levels of disorder depending on their liganded state, with the level of disorder increasing when substrates are bound.The unliganded triple mutant shows a nearly complete internal loop, with residues 176-181 disordered for the H-antigen-bound structure and 177-179 disordered for the UDP-bound structure. The UDP + H- and UDPGal + 3-deoxy-Gal inactive acceptor analog-bound structures of the triple mutant show an internal loop completely disordered over residues 176-184. The triple Cys-to-Ser mutant has an acceptor Km elevated approximately 5times over wild type, while the donor Km has doubled. Mutation shows little effect over kcat Homo sapiens
2.4.1.37 DELTA68-354 construct in which the N-terminal transmembrane domain is deleted. Deletion results in a more crystallizable protein Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.37 0.023
-
 UDP-GalNAc mutant C80S/C196S, pH 7.0, temperature not specified in the publication Homo sapiens
2.4.1.37 0.023
-
 UDP-alpha-D-galactose mutant C80S/C196S, pH 7.0, temperature not specified in the publication Homo sapiens
2.4.1.37 0.032
-
 beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide mutant C80S/C196S, pH 7.0, temperature not specified in the publication, donor UDP-alpha-D-galactose Homo sapiens
2.4.1.37 0.045
-
 UDP-GalNAc deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication Homo sapiens
2.4.1.37 0.045
-
 UDP-alpha-D-galactose deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication Homo sapiens
2.4.1.37 0.055
-
 beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication, donor UDP-alpha-D-galactose Homo sapiens
2.4.1.37 0.2
-
 beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication, donor UDP-GalNAc Homo sapiens
2.4.1.37 0.3
-
 beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication, donor UDP-GalNAc Homo sapiens
2.4.1.37 0.3
-
 beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide mutant C80S/C196S, pH 7.0, temperature not specified in the publication, donor UDP-GalNAc Homo sapiens
2.4.1.37 0.35
-
 beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication, donor UDP-alpha-D-galactose Homo sapiens
2.4.1.37 0.78
-
 UDP-GalNAc mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication Homo sapiens
2.4.1.37 0.78
-
 UDP-alpha-D-galactose mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.37 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.37 beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide + UDP-GalNAc
-
 Homo sapiens ?
-
 ?
2.4.1.37 UDP-alpha-D-galactose + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 Homo sapiens UDP + alpha-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.37 blood group B glycosyltransferase GTB
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.37 0.15
-
 beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication, co-substrate: donor UDP-GalNAc Homo sapiens
2.4.1.37 0.41
-
 beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication, co-substrate: donor UDP-GalNAc Homo sapiens
2.4.1.37 0.7
-
 beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide mutant C80S/C196S, pH 7.0, temperature not specified in the publication, co-substrate: donor UDP-GalNAc Homo sapiens
2.4.1.37 5.6
-
 beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide mutant C80S/C196S, pH 7.0, temperature not specified in the publication, co-substrate: donor UDP-alpha-D-galactose Homo sapiens
2.4.1.37 7.3
-
 beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide deletion construct with amino acids 68-354, pH 7.0, temperature not specified in the publication, co-substrate: donor UDP-alpha-D-galactose Homo sapiens
2.4.1.37 8
-
 beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide mutant C89S/C196S/C209S, pH 7.0, temperature not specified in the publication, co-substrate: donor UDP-alpha-D-galactose Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.37 7
-
 assay at Homo sapiens