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Literature summary extracted from

  • Zhao, Y.; Kim, Y.; Oh, K.; Nguyen, V.; Park, R.
    Production and characterization of extracellular chitin deacetylase from Absidia corymbifera DY-9 (2010), J. Korean Soc. Appl. Biol. Chem., 53, 119-126.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.41 acetate the enzyme is strongly inhibited by acetate with a concentration higher than 1 mM Lichtheimia corymbifera
3.5.1.41 Ag+ 70% residual activity at 10 mM Lichtheimia corymbifera
3.5.1.41 Cu2+ 14% residual activity at 10 mM Lichtheimia corymbifera
3.5.1.41 EDTA complete inhibition at 10 mM Lichtheimia corymbifera
3.5.1.41 Fe2+ 10% residual activity at 10 mM Lichtheimia corymbifera
3.5.1.41 Fe3+ 80% residual activity at 10 mM Lichtheimia corymbifera
3.5.1.41 Hg2+ complete inhibition at 10 mM Lichtheimia corymbifera
3.5.1.41 Ni2+ 24% residual activity at 10 mM Lichtheimia corymbifera
3.5.1.41 Pb2+ 6% residual activity at 10 mM Lichtheimia corymbifera
3.5.1.41 Sn2+ complete inhibition at 10 mM Lichtheimia corymbifera
3.5.1.41 Zn2+ 30% residual activity at 10 mM Lichtheimia corymbifera

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.41 extracellular
-
 Lichtheimia corymbifera
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.41 Ca2+ 164% activity at 1 mM Lichtheimia corymbifera
3.5.1.41 Co2+ 184% activity at 10 mM Lichtheimia corymbifera
3.5.1.41 Mg2+ 130% activity at 1 or 10 mM Lichtheimia corymbifera
3.5.1.41 Mn2+ 117% activity at 10 mM Lichtheimia corymbifera

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.41 Lichtheimia corymbifera
-
-
-
3.5.1.41 Lichtheimia corymbifera DY-9
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.41 chitin + H2O 19% activity compared to WSCT-50 Lichtheimia corymbifera chitosan + acetate
-
 ?
3.5.1.41 chitin + H2O 19% activity compared to WSCT-50 Lichtheimia corymbifera DY-9 chitosan + acetate
-
 ?
3.5.1.41 CM chitin + H2O 8% degree of deacetylation with 13% activity compared to water-soluble chitin with degree of deacetylation 50% Lichtheimia corymbifera ?
-
 ?
3.5.1.41 crab chitosan + H2O 71-88% degree of deacetylation with 21-10% activity compared to water-soluble chitin with degree of deacetylation 50% Lichtheimia corymbifera ?
-
 ?
3.5.1.41 di-N-acetylchitobiose + H2O 35% activity compared to hepta-N-acetylchitoheptaose Lichtheimia corymbifera ?
-
 ?
3.5.1.41 di-N-acetylchitobiose + H2O 35% activity compared to hepta-N-acetylchitoheptaose Lichtheimia corymbifera DY-9 ?
-
 ?
3.5.1.41 hepta-N-acetylchitoheptaose + H2O 100% activity Lichtheimia corymbifera ?
-
 ?
3.5.1.41 hexa-N-acetylchitohexaose + H2O 95.3% activity compared to hepta-N-acetylchitoheptaose Lichtheimia corymbifera ?
-
 ?
3.5.1.41 hexa-N-acetylchitohexaose + H2O 95.3% activity compared to hepta-N-acetylchitoheptaose Lichtheimia corymbifera DY-9 ?
-
 ?
3.5.1.41 additional information GlcNAc, colloidal chitin, regenerated chitin, beta-chitin and crystal crab chitins with different particle size are not deacetylated by the crude enzyme Lichtheimia corymbifera ?
-
 ?
3.5.1.41 additional information GlcNAc, colloidal chitin, regenerated chitin, beta-chitin and crystal crab chitins with different particle size are not deacetylated by the crude enzyme Lichtheimia corymbifera DY-9 ?
-
 ?
3.5.1.41 penta-N-acetylchitopentaose + H2O 75.7% activity compared to hepta-N-acetylchitoheptaose Lichtheimia corymbifera ?
-
 ?
3.5.1.41 penta-N-acetylchitopentaose + H2O 75.7% activity compared to hepta-N-acetylchitoheptaose Lichtheimia corymbifera DY-9 ?
-
 ?
3.5.1.41 swollen chitin + H2O 8% degree of deacetylation with 6% activity compared to water-soluble chitin with degree of deacetylation 50% Lichtheimia corymbifera ?
-
 ?
3.5.1.41 tetra-N-acetylchitotetraose + H2O 41.8% activity compared to hepta-N-acetylchitoheptaose Lichtheimia corymbifera ?
-
 ?
3.5.1.41 tri-N-acetylchitotriose + H2O 38.9% activity compared to hepta-N-acetylchitoheptaose Lichtheimia corymbifera ?
-
 ?
3.5.1.41 WSCT-50 + H2O 100% activity, water-soluble chitin with degree of deacetylation 50% Lichtheimia corymbifera ? + acetate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.1.41 CDA
-
 Lichtheimia corymbifera

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.41 55
-
-
 Lichtheimia corymbifera

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.41 20 45 the enzyme is stable below 40°C but the activity significantly declines above 45°C Lichtheimia corymbifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.41 6.5
-
-
 Lichtheimia corymbifera

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.41 8 9
-
 Lichtheimia corymbifera

Expression

EC Number Organism Comment Expression
3.5.1.41 Lichtheimia corymbifera the highest yield of enzyme is achieved in a liquid medium containing 2% powder chitin at pH 9.0, 28°C and 150 rpm up