EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.9 | DNA and amino acid sequence determination and analysis, cloning in Escherichia coli TOP10 cells, and overexpression as His6-tagged protein in Escherichia coli strain BL21 (DE3)pLysS cells using pEXP5-CT/TOPO vector. Expression in Pichia pastoris strain X-33 | Triticum aestivum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.9 | AMP | - |
Triticum aestivum | |
3.6.1.9 | EDTA | activity is restorable by Zn2+ | Triticum aestivum | |
3.6.1.9 | additional information | poor inhibition by imidazole at 2 mM | Triticum aestivum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.9 | additional information | - |
additional information | Michaelis-Menten kinetics | Triticum aestivum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.1.9 | membrane | contains a putative transmembrane domain | Triticum aestivum | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.9 | Zn2+ | activates | Triticum aestivum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.9 | Triticum aestivum | D9YT79 | cf. EC 3.1.4.1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.9 | native enzyme from wheat germs, recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography | Triticum aestivum |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.6.1.9 | 188.2 | - |
purified recombinant enzyme, pH 8.7, 35°C | Triticum aestivum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.9 | 4-nitrophenyl-5'-TMP + H2O | - |
Triticum aestivum | 4-nitrophenol + 5'-TMP | - |
? | |
3.6.1.9 | ADP-ribose + H2O | best substrate | Triticum aestivum | AMP + phosphoribose | - |
? | |
3.6.1.9 | ATP + H2O | low activity | Triticum aestivum | ADP + phosphate | - |
? | |
3.6.1.9 | CTP + H2O | low activity | Triticum aestivum | CDP + phosphate | - |
? | |
3.6.1.9 | GTP + H2O | low activity | Triticum aestivum | GDP + phosphate | - |
? | |
3.6.1.9 | NAD+ + H2O | - |
Triticum aestivum | NMN + AMP | - |
? | |
3.6.1.9 | NADH + H2O | - |
Triticum aestivum | NMNH + AMP | - |
? | |
3.6.1.9 | NADP+ + H2O | - |
Triticum aestivum | NMN + adenosine 2',5'-phosphate | - |
? | |
3.6.1.9 | NADPH + H2O | high activity | Triticum aestivum | NMNH + adenosine 2',5'-phosphate | - |
? | |
3.6.1.9 | TTP + H2O | low activity | Triticum aestivum | TDP + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.9 | oligomer | - |
Triticum aestivum |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.9 | NPP | - |
Triticum aestivum |
3.6.1.9 | nucleotide pyrophosphatase/phosphodiesterase | - |
Triticum aestivum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.9 | 70 | - |
- |
Triticum aestivum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.9 | 70 | - |
purified enzyme, pH 8.7, 30 min, stable, 75% activity remaining after 90 min | Triticum aestivum |
3.6.1.9 | 80 | - |
purified enzyme, pH 8.7, half-life is 5 min | Triticum aestivum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.9 | 12 | - |
- |
Triticum aestivum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.9 | 5 | 12 | purified enzyme, 35°C, 30 min, over 75% activity remaining | Triticum aestivum |