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Literature summary extracted from

  • Biedermannova, L.; Prokop, Z.; Gora, A.; Chovancova, E.; Kovacs, M.; Damborsky, J.; Wade, R.C.
    A single mutation in a tunnel to the active site changes the mechanism and kinetics of product release in haloalkane dehalogenase LinB (2012), J. Biol. Chem., 287, 29062-29074.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.8.1.5 L177W naturally occuring mutation, the single mutation in a tunnel to the active site changes the mechanism and kinetics of product release in LinB. Interactions of the bromide ion with the tryptophan increase free energy barrier for its passage, causing the reaction mechanism change Sphingobium indicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.8.1.5 additional information
-
 additional information steady-state and transient kinetics, kinetics of bromide ion binding, overview. Two distinct binding phases that suggest a two-step mechanism of bromide ion binding Sphingobium indicum

Organism

EC Number Organism UniProt Comment Textmining
3.8.1.5 Sphingobium indicum
-
-
-
3.8.1.5 Sphingobium indicum UT26
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.8.1.5 1-haloalkane + H2O = a primary alcohol + halide reaction mechanism, overview. The bromide release is the rate-limiting step of the catalytic cycle, overview Sphingobium indicum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.8.1.5 1-haloalkane + H2O
-
 Sphingobium indicum a primary alcohol + halide
-
 ?
3.8.1.5 1-haloalkane + H2O
-
 Sphingobium indicum UT26 a primary alcohol + halide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.8.1.5 More structural modeling using crystal structure, PDB code 1MJ5, overview Sphingobium indicum

Synonyms

EC Number Synonyms Comment Organism
3.8.1.5 LinB
-
 Sphingobium indicum

General Information

EC Number General Information Comment Organism
3.8.1.5 malfunction the single mutation in a tunnel to the active site changes the mechanism and kinetics of product release in LinB. Interactions of the bromide ion with the tryptophan increase free energy barrier for its passage, causing the reaction mechanism change Sphingobium indicum