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Literature summary extracted from

  • Buurman, E.T.; Andrews, B.; Gao, N.; Hu, J.; Keating, T.A.; Lahiri, S.; Otterbein, L.R.; Patten, A.D.; Stokes, S.S.; Shapiro, A.B.
    In vitro validation of acetyltransferase activity of GlmU as an antibacterial target in Haemophilus influenzae (2011), J. Biol. Chem., 286, 40734-40742.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.3.1.157 drug development GlmU is a target for inhibitor design Staphylococcus aureus
2.3.1.157 drug development GlmU is a target for inhibitor design Haemophilus influenzae
2.3.1.157 drug development GlmU is a target for inhibitor design Escherichia coli
2.3.1.157 drug development GlmU is a target for inhibitor design Streptococcus pneumoniae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.157 gene glmU, expression in Escherichia coli strain BL21(DE3) Staphylococcus aureus
2.3.1.157 gene glmU, expression in Escherichia coli strain BL21(DE3) Haemophilus influenzae
2.3.1.157 gene glmU, expression in Escherichia coli strain HMS174(DE3) Escherichia coli
2.3.1.157 gene glmU, expression in Escherichia coli strain HMS174(DE3) Streptococcus pneumoniae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.157 4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
-
 Escherichia coli
2.3.1.157 4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
-
 Haemophilus influenzae
2.3.1.157 4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
-
 Staphylococcus aureus
2.3.1.157 4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
-
 Streptococcus pneumoniae
2.3.1.157 4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
-
 Escherichia coli
2.3.1.157 4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
-
 Haemophilus influenzae
2.3.1.157 4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
-
 Staphylococcus aureus
2.3.1.157 4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
-
 Streptococcus pneumoniae
2.3.1.157 4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
-
 Escherichia coli
2.3.1.157 4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid substrate inhibition, competitive versus N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide Haemophilus influenzae
2.3.1.157 4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
-
 Staphylococcus aureus
2.3.1.157 4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
-
 Streptococcus pneumoniae
2.3.1.157 alpha-D-glucosamine 1-phosphate substrate inhibition, competitive versus N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide Haemophilus influenzae
2.3.1.157 additional information inhibitor design from enzyme structure, analysis of antimicrobial compounds mediating their growth inhibitory effects specifically via GlmU, antimicrobial properties of sulfonamide inhibitors of GlmU acetyl transferase, overview Escherichia coli
2.3.1.157 additional information inhibitor design from enzyme structure, analysis of antimicrobial compounds mediating their growth inhibitory effects specifically via GlmU, antimicrobial properties of sulfonamide inhibitors of GlmU acetyl transferase, overview Haemophilus influenzae
2.3.1.157 additional information inhibitor design from enzyme structure, analysis of antimicrobial compounds mediating their growth inhibitory effects specifically via GlmU, antimicrobial properties of sulfonamide inhibitors of GlmU acetyl transferase, overview Staphylococcus aureus
2.3.1.157 additional information inhibitor design from enzyme structure, analysis of antimicrobial compounds mediating their growth inhibitory effects specifically via GlmU, antimicrobial properties of sulfonamide inhibitors of GlmU acetyl transferase, overview Streptococcus pneumoniae
2.3.1.157 N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
-
 Escherichia coli
2.3.1.157 N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
-
 Haemophilus influenzae
2.3.1.157 N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
-
 Staphylococcus aureus
2.3.1.157 N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
-
 Streptococcus pneumoniae
2.3.1.157 N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
-
 Escherichia coli
2.3.1.157 N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
-
 Haemophilus influenzae
2.3.1.157 N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
-
 Staphylococcus aureus
2.3.1.157 N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
-
 Streptococcus pneumoniae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Staphylococcus aureus
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Haemophilus influenzae
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Escherichia coli
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Streptococcus pneumoniae
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Streptococcus pneumoniae NCTC 7466
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Escherichia coli ATCC 27325
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Haemophilus influenzae ATCC 51907
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate Staphylococcus aureus RN4220
-
 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.157 Escherichia coli
-
-
-
2.3.1.157 Escherichia coli ATCC 27325
-
-
-
2.3.1.157 Haemophilus influenzae
-
-
-
2.3.1.157 Haemophilus influenzae ATCC 51907
-
-
-
2.3.1.157 Staphylococcus aureus
-
-
-
2.3.1.157 Staphylococcus aureus RN4220
-
-
-
2.3.1.157 Streptococcus pneumoniae
-
-
-
2.3.1.157 Streptococcus pneumoniae NCTC 7466
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.157 recombinant GlmU from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration Staphylococcus aureus
2.3.1.157 recombinant GlmU from Escherichia coli strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration Haemophilus influenzae
2.3.1.157 recombinant GlmU from Escherichia coli strain HMS174(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration Escherichia coli
2.3.1.157 recombinant GlmU from Escherichia coli strain HMS174(DE3) by anion exchange and hydrophobic interaction chromatography and gel filtration Streptococcus pneumoniae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Staphylococcus aureus CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Haemophilus influenzae CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Escherichia coli CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Streptococcus pneumoniae CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Streptococcus pneumoniae NCTC 7466 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Escherichia coli ATCC 27325 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Haemophilus influenzae ATCC 51907 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?
2.3.1.157 acetyl-CoA + alpha-D-glucosamine 1-phosphate
-
 Staphylococcus aureus RN4220 CoA + N-acetyl-alpha-D-glucosamine 1-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.157 GlmU
-
 Staphylococcus aureus
2.3.1.157 GlmU
-
 Haemophilus influenzae
2.3.1.157 GlmU
-
 Escherichia coli
2.3.1.157 GlmU
-
 Streptococcus pneumoniae
2.3.1.157 GlmU acetyltransferase
-
 Staphylococcus aureus
2.3.1.157 GlmU acetyltransferase
-
 Haemophilus influenzae
2.3.1.157 GlmU acetyltransferase
-
 Escherichia coli
2.3.1.157 GlmU acetyltransferase
-
 Streptococcus pneumoniae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.157 7.2
-
 assay at Staphylococcus aureus
2.3.1.157 7.2
-
 assay at Haemophilus influenzae
2.3.1.157 7.2
-
 assay at Escherichia coli
2.3.1.157 7.2
-
 assay at Streptococcus pneumoniae

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.157 additional information
-
 additional information inhibition kinetics, ping pong bisubstrate rate, overview Haemophilus influenzae

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.157 0.000007
-
 pH 7.2, temperature not specified in the publication Escherichia coli 4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
2.3.1.157 0.000018
-
 pH 7.2, temperature not specified in the publication Escherichia coli 4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
2.3.1.157 0.000023
-
 pH 7.2, temperature not specified in the publication Haemophilus influenzae 4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
2.3.1.157 0.000058
-
 pH 7.2, temperature not specified in the publication Escherichia coli 4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
2.3.1.157 0.00028
-
 pH 7.2, temperature not specified in the publication Haemophilus influenzae 4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
2.3.1.157 0.0004
-
 pH 7.2, temperature not specified in the publication Haemophilus influenzae 4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
2.3.1.157 0.0021
-
 pH 7.2, temperature not specified in the publication Escherichia coli N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
2.3.1.157 0.0033
-
 pH 7.2, temperature not specified in the publication Escherichia coli N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
2.3.1.157 0.0047
-
 pH 7.2, temperature not specified in the publication Streptococcus pneumoniae 4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid
2.3.1.157 0.0064
-
 pH 7.2, temperature not specified in the publication Haemophilus influenzae N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
2.3.1.157 0.0078
-
 pH 7.2, temperature not specified in the publication Haemophilus influenzae N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
2.3.1.157 0.015
-
 pH 7.2, temperature not specified in the publication Streptococcus pneumoniae 4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
2.3.1.157 0.04
-
 pH 7.2, temperature not specified in the publication Streptococcus pneumoniae 4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
2.3.1.157 0.2
-
 above, pH 7.2, temperature not specified in the publication Staphylococcus aureus N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
2.3.1.157 0.2
-
 above, pH 7.2, temperature not specified in the publication Streptococcus pneumoniae N-[2,4-dimethoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]acetamide
2.3.1.157 0.2
-
 above, pH 7.2, temperature not specified in the publication Staphylococcus aureus N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
2.3.1.157 0.2
-
 above, pH 7.2, temperature not specified in the publication Streptococcus pneumoniae N-[2,4-dimethoxy-5-[methyl(phenyl)sulfamoyl]phenyl]acetamide
2.3.1.157 0.2
-
 above, pH 7.2, temperature not specified in the publication Staphylococcus aureus 4-[[2,4-dimethoxy-5-(10H-phenoxazin-10-ylsulfonyl)phenyl]amino]-4-oxobutanoic acid
2.3.1.157 0.2
-
 above, pH 7.2, temperature not specified in the publication Staphylococcus aureus 4-([5-[(4-aminophenyl)(phenyl)sulfamoyl]-2,4-dimethoxyphenyl]amino)-4-oxobutanoic acid
2.3.1.157 0.2
-
 above, pH 7.2, temperature not specified in the publication Staphylococcus aureus 4-([4-hydroxy-2-methoxy-5-[(2-methyl-3,4-dihydroquinolin-1(2H)-yl)sulfonyl]phenyl]amino)-4-oxobutanoic acid

General Information

EC Number General Information Comment Organism
2.3.1.157 physiological function GlmU is a bifunctional enzyme that is essential for bacterial growth, converting D-glucosamine 1-phosphate into UDPGlcNAc via acetylation and subsequent uridyl transfer Staphylococcus aureus
2.3.1.157 physiological function GlmU is a bifunctional enzyme that is essential for bacterial growth, converting D-glucosamine 1-phosphate into UDPGlcNAc via acetylation and subsequent uridyl transfer Haemophilus influenzae
2.3.1.157 physiological function GlmU is a bifunctional enzyme that is essential for bacterial growth, converting D-glucosamine 1-phosphate into UDPGlcNAc via acetylation and subsequent uridyl transfer Escherichia coli
2.3.1.157 physiological function GlmU is a bifunctional enzyme that is essential for bacterial growth, converting D-glucosamine 1-phosphate into UDPGlcNAc via acetylation and subsequent uridyl transfer Streptococcus pneumoniae