EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.163 | 6-hydroxy-3-succinoylpyridine | acts as an initial effector and strongly stimulates NADH oxidation | Pseudomonas putida |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.13.163 | additional information | deletion of a GADGA motif in the FAD binding-domain results in complete loss of activity in the mutant protein | Pseudomonas putida |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.163 | 0.104 | - |
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate | pH 8.0, 25°C | Pseudomonas putida | |
1.14.13.163 | 0.175 | - |
NADH | pH 8.0, 25°C | Pseudomonas putida |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.163 | Cu2+ | 2 mM, complete loss of activity | Pseudomonas putida | |
1.14.13.163 | Zn2+ | 2 mM, complete loss of activity | Pseudomonas putida |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.14.13.163 | 40000 | - |
2 * 40000, SDS-PAGE | Pseudomonas putida |
1.14.13.163 | 80000 | - |
PAGE | Pseudomonas putida |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.13.163 | Pseudomonas putida | F8G0M4 | - |
- |
1.14.13.163 | Pseudomonas putida S16 | F8G0M4 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.14.13.163 | - |
Pseudomonas putida |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.14.13.163 | 4.8 | - |
pH 8.0, 25°C | Pseudomonas putida |
EC Number | Storage Stability | Organism |
---|---|---|
1.14.13.163 | -20°C, Tris-HCl buffer, stable for several months | Pseudomonas putida |
1.14.13.163 | 4°C Tris-HCl buffer, stable for at least one week | Pseudomonas putida |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.13.163 | 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 | - |
Pseudomonas putida | 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O | - |
? | |
1.14.13.163 | 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2 | - |
Pseudomonas putida S16 | 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.14.13.163 | dimer | 2 * 40000, SDS-PAGE | Pseudomonas putida |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.14.13.163 | HSP hydroxylase | - |
Pseudomonas putida |
1.14.13.163 | hspB | - |
Pseudomonas putida |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.163 | 25 | - |
- |
Pseudomonas putida |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.14.13.163 | 25 | - |
30 min, stable up to | Pseudomonas putida |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.14.13.163 | 2 | - |
NADH | pH 8.0, 25°C | Pseudomonas putida |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.163 | 8 | - |
- |
Pseudomonas putida |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.14.13.163 | 7 | 9 | incubation overnight, 20% loss of activity | Pseudomonas putida |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.14.13.163 | FAD | - |
Pseudomonas putida | |
1.14.13.163 | additional information | no cofactor: NADPH | Pseudomonas putida | |
1.14.13.163 | NADH | specific for NADH | Pseudomonas putida |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.14.13.163 | physiological function | gene is essential for nicotine degradation | Pseudomonas putida |