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Literature summary extracted from

  • Tang, H.; Yao, Y.; Zhang, D.; Meng, X.; Wang, L.; Yu, H.; Ma, L.; Xu, P.
    A novel NADH-dependent and FAD-containing hydroxylase is crucial for nicotine degradation by Pseudomonas putida (2011), J. Biol. Chem., 286, 39179-39187.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.163 6-hydroxy-3-succinoylpyridine acts as an initial effector and strongly stimulates NADH oxidation Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.163 additional information deletion of a GADGA motif in the FAD binding-domain results in complete loss of activity in the mutant protein Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.163 0.104
-
4-(6-hydroxypyridin-3-yl)-4-oxobutanoate pH 8.0, 25°C Pseudomonas putida
1.14.13.163 0.175
-
NADH pH 8.0, 25°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.163 Cu2+ 2 mM, complete loss of activity Pseudomonas putida
1.14.13.163 Zn2+ 2 mM, complete loss of activity Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.163 40000
-
2 * 40000, SDS-PAGE Pseudomonas putida
1.14.13.163 80000
-
PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.163 Pseudomonas putida F8G0M4
-
-
1.14.13.163 Pseudomonas putida S16 F8G0M4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.163
-
Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.163 4.8
-
pH 8.0, 25°C Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.14.13.163 -20°C, Tris-HCl buffer, stable for several months Pseudomonas putida
1.14.13.163 4°C Tris-HCl buffer, stable for at least one week Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.163 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
-
Pseudomonas putida 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
?
1.14.13.163 4-(6-hydroxypyridin-3-yl)-4-oxobutanoate + 2 NADH + 2 H+ + O2
-
Pseudomonas putida S16 2,5-dihydroxypyridine + succinate semialdehyde + 2 NAD+ + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.14.13.163 dimer 2 * 40000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.13.163 HSP hydroxylase
-
Pseudomonas putida
1.14.13.163 hspB
-
Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.163 25
-
-
Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.13.163 25
-
30 min, stable up to Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.163 2
-
NADH pH 8.0, 25°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.163 8
-
-
Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.13.163 7 9 incubation overnight, 20% loss of activity Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.163 FAD
-
Pseudomonas putida
1.14.13.163 additional information no cofactor: NADPH Pseudomonas putida
1.14.13.163 NADH specific for NADH Pseudomonas putida

General Information

EC Number General Information Comment Organism
1.14.13.163 physiological function gene is essential for nicotine degradation Pseudomonas putida