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Literature summary extracted from

  • Borisova, S.A.; Christman, H.D.; Metcalf, M.E.; Zulkepli, N.A.; Zhang, J.K.; van der Donk, W.A.; Metcalf, W.W.
    Genetic and biochemical characterization of a pathway for the degradation of 2-aminoethylphosphonate in Sinorhizobium meliloti 1021 (2011), J. Biol. Chem., 286, 22283-22290.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.3 ascorbate required Sinorhizobium meliloti

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.3 expressed in Escherichia coli BL21(DE3) cells Sinorhizobium meliloti
3.11.1.2 gene phnA, expression of N-terminally His6-tagged PhnA in Escherichia coli Sinorhizobium meliloti

Protein Variants

EC Number Protein Variants Comment Organism
3.11.1.2 additional information construction of a phnA deletion mutant Sinorhizobium meliloti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.3 0.5
-
 phosphonoacetaldehyde at pH 7.5 and 30°C Sinorhizobium meliloti
1.2.1.3 1
-
 NAD+ at pH 7.5 and 30°C Sinorhizobium meliloti
3.11.1.2 additional information
-
 additional information steady-state kinetics analysis Sinorhizobium meliloti
3.11.1.2 0.0221
-
 Phosphonoacetate pH 7.5, 30°C, recombinant enzyme Sinorhizobium meliloti

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.3 Fe2+ required Sinorhizobium meliloti
3.11.1.2 Zn2+ increases kcat and Km, decreasing kcat/KM, in the reaction Sinorhizobium meliloti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.3 55076
-
 x * 55076, His-tagged enzyme, calculated from amino acid sequence Sinorhizobium meliloti
1.2.1.3 55100
-
 x * 55100, His-tagged enzyme, SDS-PAGE Sinorhizobium meliloti
3.11.1.2 48400
-
 x * 48400, recombinant His6-tagged PhnA, SDS-PAGE Sinorhizobium meliloti

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.3 phosphonoacetaldehyde + NAD+ Sinorhizobium meliloti
-
 ?
-
 ?
3.11.1.2 Phosphonoacetate + H2O Sinorhizobium meliloti
-
 acetate + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.3 Sinorhizobium meliloti
-
-
-
3.11.1.2 Sinorhizobium meliloti
-
 gene phnA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.3 Ni-NTA resin column chromatography, and gel filtration Sinorhizobium meliloti
3.11.1.2 recombinant N-terminally His6-tagged PhnA from Escherichia coli by nickel affinity chromatography Sinorhizobium meliloti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.3 phosphonoacetaldehyde + NAD+
-
 Sinorhizobium meliloti ?
-
 ?
3.11.1.2 Phosphonoacetate + H2O
-
 Sinorhizobium meliloti acetate + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.3 ? x * 55076, His-tagged enzyme, calculated from amino acid sequence Sinorhizobium meliloti
1.2.1.3 ? x * 55100, His-tagged enzyme, SDS-PAGE Sinorhizobium meliloti
3.11.1.2 ? x * 48400, recombinant His6-tagged PhnA, SDS-PAGE Sinorhizobium meliloti

Synonyms

EC Number Synonyms Comment Organism
1.2.1.3 phnY
-
 Sinorhizobium meliloti
1.2.1.3 phosphonoacetaldehyde dehydrogenase
-
 Sinorhizobium meliloti
1.2.1.3 PnAA dehydrogenase
-
 Sinorhizobium meliloti
3.11.1.2 PhnA
-
 Sinorhizobium meliloti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.11.1.2 30
-
 assay at Sinorhizobium meliloti

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.11.1.2 0.9
-
 Phosphonoacetate pH 7.5, 30°C, recombinant enzyme Sinorhizobium meliloti
3.11.1.2 1.1
-
 Phosphonoacetate pH 7.5, 30°C, recombinant enzyme, with 0.01 mM Zn2+ Sinorhizobium meliloti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.11.1.2 7.5
-
 assay at Sinorhizobium meliloti

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.3 NAD+
-
 Sinorhizobium meliloti

General Information

EC Number General Information Comment Organism
3.11.1.2 metabolism the enzyme catalyzes a step in the 2-aminoethylphosphonate degradation, conversion of 2-aminoethylphosphonate to phosphate and degradation of phosphonate compounds, pathway overview Sinorhizobium meliloti

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.11.1.2 4100
-
 Phosphonoacetate pH 7.5, 30°C, recombinant enzyme Sinorhizobium meliloti