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Literature summary extracted from

  • Lendrihas, T.; Hunter, G.A.; Ferreira, G.C.
    Targeting the active site gate to yield hyperactive variants of 5-aminolevulinate synthase (2010), J. Biol. Chem., 285, 13704-13711.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.37 A425G/Y428H/R433H/G434N/E435K/L437K hexa variant, kcat increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type Escherichia coli
2.3.1.37 A425T single variant, kcat slightly increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type Escherichia coli
2.3.1.37 L437Q single variant, kcat comparable to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type Escherichia coli
2.3.1.37 N427H single variant, kcat slightly increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type Escherichia coli
2.3.1.37 R433K/G434K/E435Q/L437Q quadruple variant, kcat increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type Escherichia coli
2.3.1.37 V423I/A425P/Y428C/P432R/R433K/E435N hexa variant, kcat increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type Escherichia coli
2.3.1.37 V423L/Y428R/P432E/R433I/G434N/E435Q/L437K hepta variant, kcat increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type Escherichia coli
2.3.1.37 Y428I/R433Q /G434N/E435T/L437N penta variant, kcat increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type Escherichia coli
2.3.1.37 Y428N/P432N/R433I/G434E/E435K/L437K hexa variant, kcat increased compared to wild-type, Km (Gly) comparable to wild-type, Km (succinyl-CoA) decreased compared to wild-type Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.37 0.001
-
 glycine mutant Y428I/R433Q /G434N/E435T/L437N, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.0015
-
 glycine mutant N427H, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.0017
-
 glycine mutant L437Q, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.002
-
 glycine mutant Y428N/P432N/R433I/G434E/E435K/L437K, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.0023
-
 glycine mutant A425T, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.0023
-
 glycine mutant V423I/A425P/Y428C/P432R/R433K/E435N, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.0026
-
 glycine mutant A425G/Y428H/R433H/G434N/E435K/L437K, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.0029
-
 glycine mutant R433K/G434K/E435Q/L437Q, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.003
-
 glycine mutant V423L/Y428R/P432E/R433I/G434N/E435Q/L437K, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.011
-
 glycine wild-type, pH 7.5, 20°C Escherichia coli
2.3.1.37 12
-
 glycine mutant V423L/Y428R/P432E/R433I/G434N/E435Q/L437K, pH 7.5, 20°C Escherichia coli
2.3.1.37 13
-
 glycine mutant A425G/Y428H/R433H/G434N/E435K/L437K, pH 7.5, 20°C Escherichia coli
2.3.1.37 13
-
 glycine mutant Y428N/P432N/R433I/G434E/E435K/L437K, pH 7.5, 20°C Escherichia coli
2.3.1.37 14
-
 glycine mutant V423I/A425P/Y428C/P432R/R433K/E435N, pH 7.5, 20°C Escherichia coli
2.3.1.37 14
-
 glycine wild-type, pH 7.5, 20°C Escherichia coli
2.3.1.37 15
-
 glycine mutant N427H, pH 7.5, 20°C Escherichia coli
2.3.1.37 16
-
 glycine mutant R433K/G434K/E435Q/L437Q, pH 7.5, 20°C Escherichia coli
2.3.1.37 16
-
 glycine mutant Y428I/R433Q /G434N/E435T/L437N, pH 7.5, 20°C Escherichia coli
2.3.1.37 24
-
 glycine mutant L437Q, pH 7.5, 20°C Escherichia coli
2.3.1.37 25
-
 glycine mutant A425T, pH 7.5, 20°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.37 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.37 succinyl-CoA + glycine
-
 Escherichia coli 5-aminolevulinate + CoA + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.37 ALAS
-
 Escherichia coli
2.3.1.37 sigma-aminolevulinate synthase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.37 20
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.37 0.02
-
 glycine wild-type, co-substrate: succinyl-CoA, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.03
-
 glycine co-substrate: succinyl-CoA, mutant L437Q, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.07
-
 glycine co-substrate: succinyl-CoA, mutant A425T, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.07
-
 glycine co-substrate: succinyl-CoA, mutant N427H, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.16
-
 glycine co-substrate: succinyl-CoA, mutant V423I/A425P/Y428C/P432R/R433K/E435N, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.17
-
 glycine co-substrate: succinyl-CoA, mutant Y428I/R433Q /G434N/E435T/L437N, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.2
-
 glycine co-substrate: succinyl-CoA, mutant A425G/Y428H/R433H/G434N/E435K/L437K, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.2
-
 glycine co-substrate: succinyl-CoA, mutant R433K/G434K/E435Q/L437Q, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.23
-
 glycine co-substrate: succinyl-CoA, mutant Y428N/P432N/R433I/G434E/E435K/L437K, pH 7.5, 20°C Escherichia coli
2.3.1.37 0.31
-
 glycine co-substrate: succinyl-CoA, mutant V423L/Y428R/P432E/R433I/G434N/E435Q/L437K, pH 7.5, 20°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.37 7.5
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.3.1.37 pyridoxal 5'-phosphate
-
 Escherichia coli