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Literature summary extracted from

  • Phillips, G.; Chikwana, V.; Maxwell, A.; El-Yacoubi, B.; Swairjo, M.; Iwata-Reuyl, D.; De Crecy-Lagard, V.
    Discovery and characterization of an amidinotransferase involved in the modification of archaeal tRNA (2010), J. Biol. Chem., 285, 12706-12713.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.97 gene mj1022 Methanocaldococcus jannaschii
2.6.1.97 gene tgtA2 Haloferax volcanii

Protein Variants

EC Number Protein Variants Comment Organism
2.6.1.97 additional information construction of and genotype and phenotype of the DELTA tgtA2 strain VDC5203 Haloferax volcanii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O Haloferax volcanii
-
 L-glutamate + archaeine15 in tRNA
-
 ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O Methanocaldococcus jannaschii
-
 L-glutamate + archaeine15 in tRNA
-
 ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O Methanocaldococcus jannaschii DSM 2661
-
 L-glutamate + archaeine15 in tRNA
-
 ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O Haloferax volcanii H26
-
 L-glutamate + archaeine15 in tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.97 Haloferax volcanii
-
 gene tgtA2
-
2.6.1.97 Haloferax volcanii H26
-
 gene tgtA2
-
2.6.1.97 Methanocaldococcus jannaschii Q58428 gene mj1022
-
2.6.1.97 Methanocaldococcus jannaschii DSM 2661 Q58428 gene mj1022
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
 Haloferax volcanii L-glutamate + archaeine15 in tRNA
-
 ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
 Methanocaldococcus jannaschii L-glutamate + archaeine15 in tRNA
-
 ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O preQ0-tRNASer is used as substrate Haloferax volcanii L-glutamate + archaeine15 in tRNA
-
 ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O preQ0-tRNASer is used as substrate Methanocaldococcus jannaschii L-glutamate + archaeine15 in tRNA
-
 ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
 Methanocaldococcus jannaschii DSM 2661 L-glutamate + archaeine15 in tRNA
-
 ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O preQ0-tRNASer is used as substrate Methanocaldococcus jannaschii DSM 2661 L-glutamate + archaeine15 in tRNA
-
 ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O
-
 Haloferax volcanii H26 L-glutamate + archaeine15 in tRNA
-
 ?
2.6.1.97 L-glutamine + 7-cyano-7-carbaguanine15 in tRNA + H2O preQ0-tRNASer is used as substrate Haloferax volcanii H26 L-glutamate + archaeine15 in tRNA
-
 ?
2.6.1.97 additional information recombinant MjTgtA2 converts preQ0-tRNA to G+-tRNA using several nitrogen sources and to do so in an ATP-independent process. MjTgtA2 shows in vitro glutaminase activity Methanocaldococcus jannaschii ?
-
 ?
2.6.1.97 additional information recombinant MjTgtA2 converts preQ0-tRNA to G+-tRNA using several nitrogen sources and to do so in an ATP-independent process. MjTgtA2 shows in vitro glutaminase activity Methanocaldococcus jannaschii DSM 2661 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.6.1.97 TgtA2
-
 Methanocaldococcus jannaschii
2.6.1.97 TgtA2
-
 Haloferax volcanii
2.6.1.97 tRNA-guanine transglycosylase
-
 Haloferax volcanii
2.6.1.97 tRNA-guanine transglycosylase
-
 Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.97 7
-
 glutaminase activity assay at Methanocaldococcus jannaschii

General Information

EC Number General Information Comment Organism
2.6.1.97 evolution structure-based alignments comparing arcTGT and TgtA2 reveal that TgtA2 lacks key arcTGT catalytic residues and contains an additional module. Members of the TgtA2 and arcTGT family do not perfectly co-distribute Haloferax volcanii
2.6.1.97 evolution structure-based alignments comparing arcTGT and TgtA2 reveal that TgtA2 lacks key arcTGT catalytic residues and contains an additional module. Members of the TgtA2 and arcTGT family do not perfectly co-distribute Methanocaldococcus jannaschii
2.6.1.97 malfunction a Haloferax volcanii DELTAtgtA2 derivative demonstrates that tRNA from the mutant strain lacks G+ and instead accumulates preQ0 Haloferax volcanii
2.6.1.97 malfunction a Haloferax volcanii DELTAtgtA2 derivative demonstrates that tRNA from the mutant strain lacks G+ and instead accumulates preQ0 Methanocaldococcus jannaschii
2.6.1.97 metabolism TgtA2 is involved in archaeosine biosynthesis in vivo. Archaeosine biosynthesis is especially complex, involving the initial production of 7-cyano-7-deazaguanine (preQ0), an advanced precursor that is produced in a tRNA-independent portion of the biosynthesis, followed by its insertion into the tRNA by the enzyme tRNA-guanine transglycosylase, which replaces the target guanine base yielding preQ0-tRNA Haloferax volcanii
2.6.1.97 metabolism TgtA2 is involved in archaeosine biosynthesis in vivo. Archaeosine biosynthesis is especially complex, involving the initial production of 7-cyano-7-deazaguanine (preQ0), an advanced precursor that is produced in a tRNA-independent portion of the biosynthesis, followed by its insertion into the tRNA by the enzyme tRNA-guanine transglycosylase, which replaces the target guanine base yielding preQ0-tRNA Methanocaldococcus jannaschii
2.6.1.97 additional information presence of the 7-deazaguanosine derivative archaeosine, i.e. G+, at position 15 in tRNA is one of the diagnostic molecular characteristics of the archaea Haloferax volcanii
2.6.1.97 additional information presence of the 7-deazaguanosine derivative archaeosine, i.e. G+, at position 15 in tRNA is one of the diagnostic molecular characteristics of the archaea Methanocaldococcus jannaschii