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Literature summary extracted from

  • Katsuyama, Y.; Kita, T.; Funa, N.; Horinouchi, S.
    Curcuminoid biosynthesis by two type III polyketide synthases in the herb Curcuma longa (2009), J. Biol. Chem., 284, 11160-11170.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.217 DNA and amino acid sequence determination and analysis, expression analysis by quantitative real-time PCR, recombinant expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Curcuma longa
2.3.1.218 DNA and amino acid sequence determination and analysis, expression analysis by quantitative real-time PCR, recombinant expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Curcuma longa
2.3.1.219 DNA and amino acid sequence determination and analysis, expression analysis by quantitative real-time PCR, recombinant expression as N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Curcuma longa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.217 additional information
-
 additional information kinetic analysis of CURS, overview Curcuma longa
2.3.1.217 0.018
-
 feruloyl-CoA pH 8.0, 37°C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA Curcuma longa
2.3.1.218 additional information
-
 additional information DCS is an allosteric enzyme. Homotropic activation decreases the activity as the substrate concentration decreases more quickly than those exhibiting Michaelis-Menten-type kinetics. Kinetic analysis of DCS, overview Curcuma longa
2.3.1.218 0.0084
-
 malonyl-CoA pH 7.5, 37°C, recombinant enzyme Curcuma longa
2.3.1.219 additional information
-
 additional information kinetic analysis of CURS, overview Curcuma longa
2.3.1.219 0.085
-
 4-coumaroyl-CoA pH 8.0, 37°C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA Curcuma longa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.217 feruloyl-CoA + feruloylacetyl-CoA + H2O Curcuma longa
-
 2 CoA + curcumin + CO2
-
 ?
2.3.1.218 4-coumaroyl-CoA + malonyl-CoA Curcuma longa
-
 (4-coumaroyl)acetyl-CoA + CO2 + CoA
-
 ?
2.3.1.218 feruloyl-CoA + malonyl-CoA Curcuma longa
-
 feruloylacetyl-CoA + CO2 + CoA
-
 ?
2.3.1.219 (4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O Curcuma longa
-
 bisdemethoxycurcumin + CO2 + CoA
-
 ?
2.3.1.219 (4-coumaroyl)acetyl-CoA + feruloyl-CoA + H2O Curcuma longa
-
 demethoxycurcumin + CO2 + CoA
-
 ?
2.3.1.219 4-coumaroyl-CoA + feruloylacetyl-CoA Curcuma longa
-
 demethoxycurcumin + CO2 + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.217 Curcuma longa
-
-
-
2.3.1.218 Curcuma longa
-
-
-
2.3.1.219 Curcuma longa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.217 leaf
-
 Curcuma longa
-
2.3.1.217 rhizome
-
 Curcuma longa
-
2.3.1.218 leaf
-
 Curcuma longa
-
2.3.1.218 rhizome
-
 Curcuma longa
-
2.3.1.219 leaf
-
 Curcuma longa
-
2.3.1.219 rhizome
-
 Curcuma longa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.217 cinnamoyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O low activity Curcuma longa CoA + dicinnamoylmethane + N-acetylcysteamine
-
 ?
2.3.1.217 feruloyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O
-
 Curcuma longa CoA + cinnamoylferuloylmethane + N-acetylcysteamine + CO2
-
 ?
2.3.1.217 feruloyl-CoA + feruloylacetyl-CoA + H2O
-
 Curcuma longa 2 CoA + curcumin + CO2
-
 ?
2.3.1.217 feruloyl-CoA + feruloylacetyl-CoA + H2O feruloyl-CoA is the preferred starter substrate Curcuma longa 2 CoA + curcumin + CO2 product identification by LC-ESI MS/MS ?
2.3.1.217 additional information the enzyme can also convert diketide-CoA esters into 2-oxoacids and CoA, which are then converted into curcuminoids. For activity assays, cinnamoyl-CoA, 4-coumaroyl-CoA, or feruloyl-CoA are used as starter substrates and malonyl-CoA or cinnamoyl-diketide-N-acetylcysteamine as extender substrates, cf. EC 2.3.1.218 and EC 2.31.219 Curcuma longa ?
-
 ?
2.3.1.218 4-coumaroyl-CoA + malonyl-CoA
-
 Curcuma longa (4-coumaroyl)acetyl-CoA + CO2 + CoA
-
 ?
2.3.1.218 feruloyl-CoA + malonyl-CoA
-
 Curcuma longa feruloylacetyl-CoA + CO2 + CoA
-
 ?
2.3.1.218 feruloyl-CoA + malonyl-CoA
-
 Curcuma longa feruloylacetyl-CoA + CO2 + CoA product identification by LC-ESI MS/MS ?
2.3.1.219 (4-coumaroyl)acetyl-CoA + 4-coumaroyl-CoA + H2O
-
 Curcuma longa bisdemethoxycurcumin + CO2 + CoA
-
 ?
2.3.1.219 (4-coumaroyl)acetyl-CoA + feruloyl-CoA + H2O
-
 Curcuma longa demethoxycurcumin + CO2 + CoA
-
 ?
2.3.1.219 4-coumaroyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O
-
 Curcuma longa CoA + cinnamoyl-4-coumaroylmethane + N-acetylcysteamine
-
 ?
2.3.1.219 4-coumaroyl-CoA + feruloylacetyl-CoA
-
 Curcuma longa demethoxycurcumin + CO2 + CoA
-
 ?
2.3.1.219 cinnamoyl-CoA + cinnamoyl-diketide-N-acetylcysteamine + H2O low activity Curcuma longa CoA + dicinnamoylmethane + N-acetylcysteamine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.217 curcumin synthase
-
 Curcuma longa
2.3.1.217 CURS
-
 Curcuma longa
2.3.1.217 CURS1
-
 Curcuma longa
2.3.1.217 CURS2
-
 Curcuma longa
2.3.1.217 CURS3
-
 Curcuma longa
2.3.1.218 DCS
-
 Curcuma longa
2.3.1.218 diketide-CoA synthase
-
 Curcuma longa
2.3.1.218 phenylpropanoyl-diketide-CoA synthase
-
 Curcuma longa
2.3.1.219 curcumin synthase
-
 Curcuma longa
2.3.1.219 CURS
-
 Curcuma longa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.217 37
-
 assay at Curcuma longa
2.3.1.218 37
-
 assay at Curcuma longa
2.3.1.219 37
-
 assay at Curcuma longa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.3.1.217 0.0183
-
 feruloyl-CoA pH 8.0, 37°C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA Curcuma longa
2.3.1.218 0.0112
-
 malonyl-CoA pH 7.5, 37°C, recombinant enzyme Curcuma longa
2.3.1.218 0.02
-
 feruloyl-CoA pH 7.5, 37°C, recombinant enzyme Curcuma longa
2.3.1.219 3.15
-
 4-coumaroyl-CoA pH 8.0, 37°C, with cinnamoyl-diketide-N-acetylcysteaminyl-CoA Curcuma longa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.217 8
-
 assay at Curcuma longa
2.3.1.218 7.5
-
 assay at Curcuma longa
2.3.1.219 8
-
 assay at Curcuma longa

General Information

EC Number General Information Comment Organism
2.3.1.217 evolution the enzyme belongs to the Type III polyketide synthase family Curcuma longa
2.3.1.217 metabolism the enzyme catalyzes a step in the curcuminoid biosynthesis, pathway overview Curcuma longa
2.3.1.217 additional information CURS can collaborate with curcumin synthase, DCS, EC 2.3.1.218, which provides the substrate feruloylacetyl-CoA from condensation of feruloyl-CoA and malonyl-CoA, overview Curcuma longa
2.3.1.218 evolution the enzyme belongs to the Type III polyketide synthase family, but DCS is a unique type III PKS that releases the product as a CoA-bound form Curcuma longa
2.3.1.218 metabolism the enzyme catalyzes a step in the curcuminoid biosynthesis, pathway overview Curcuma longa
2.3.1.218 additional information DCS can collaborate with curcumin synthase, CURS, EC 2.3.1.217, which uses the reaction product feruloylacetyl-CoA for formation of curcumin, overview Curcuma longa
2.3.1.219 evolution the enzyme belongs to the Type III polyketide synthase family Curcuma longa
2.3.1.219 metabolism the enzyme catalyzes a step in the curcuminoid biosynthesis, pathway overview Curcuma longa
2.3.1.219 additional information CURS can collaborate with curcumin synthase, DCS, EC 2.3.1.218, which provides the substrate feruloylacetyl-CoA from condensation of feruloyl-CoA and malonyl-CoA, overview Curcuma longa