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Literature summary extracted from

  • Cacciapuoti, G.; Porcelli, M.; Moretti, M.A.; Sorrentino, F.; Concilio, L.; Zappia, V.; Liu, Z.J.; Tempel, W.; Schubot, F.; Rose, J.P.; Wang, B.C.; Brereton, P.S.; Jenney, F.E.; Adams, M.W.
    The first agmatine/cadaverine aminopropyl transferase: biochemical and structural characterization of an enzyme involved in polyamine biosynthesis in the hyperthermophilic archaeon Pyrococcus furiosus (2007), J. Bacteriol., 189, 6057-6067.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.104 expression in Escherichia coli Pyrococcus furiosus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.5.1.104 sitting drop vapor diffusion method, 1.8 A resolution Pyrococcus furiosus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.104 0.0076
-
 agmatine pH 7.4, 70°C Pyrococcus furiosus
2.5.1.104 1.88
-
 propane-1,3-diamine pH 7.4, 70°C Pyrococcus furiosus
2.5.1.104 3.77
-
 cadaverine pH 7.4, 70°C Pyrococcus furiosus
2.5.1.104 7.7
-
 putrescine pH 7.4, 70°C Pyrococcus furiosus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.104 32334
-
 2 * 32334, calculated from sequence Pyrococcus furiosus
2.5.1.104 32600
-
 2 * 32600, SDS-PAGE Pyrococcus furiosus
2.5.1.104 65000
-
 gel filtration Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + agmatine Pyrococcus furiosus agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
 ?
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + cadaverine Pyrococcus furiosus agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required 5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.104 Pyrococcus furiosus Q8U4G1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.104
-
 Pyrococcus furiosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + agmatine agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
 ?
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + agmatine 4.9% of the activity compared to cadaverine Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + N1-aminopropylagmatine
-
 ?
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + cadaverine
-
 Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
 ?
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + cadaverine agmatine is the physiological substrate in the constitutive synthesis of spermidine, while cadaverine acts as an aminopropyl acceptor under stress conditions when an active and rapid synthesis of N-(3-aminopropyl)cadaverine could be required Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + N-(3-aminopropyl)cadaverine
-
 ?
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + propane-1,3-diamine 2.9% of the activity compared to cadaverine Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + bis(3-aminopropyl)amine
-
 ?
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + putrescine 8.7% of the activity compared to cadaverine Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + spermidine
-
 ?
2.5.1.104 S-adenosyl 3-(methylthio)propylamine + spermidine 0.8% of the activity compared to cadaverine Pyrococcus furiosus 5'-S-methyl-5'-thioadenosine + spermine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.104 homodimer 2 * 32600, SDS-PAGE Pyrococcus furiosus
2.5.1.104 homodimer 2 * 32334, calculated from sequence Pyrococcus furiosus

Synonyms

EC Number Synonyms Comment Organism
2.5.1.104 ACAPT
-
 Pyrococcus furiosus
2.5.1.104 agmatine/cadaverine aminopropyl transferase
-
 Pyrococcus furiosus
2.5.1.104 PF0127
-
 Pyrococcus furiosus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.104 70
-
 assay at Pyrococcus furiosus
2.5.1.104 90
-
-
 Pyrococcus furiosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.5.1.104 70 103 70°C: about 50% of maximal activity, 103°C: about 50% of maximal activity Pyrococcus furiosus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.104 108
-
 transition temperature Pyrococcus furiosus
2.5.1.104 112
-
 transition temperature in presence of 5 mM cadaverine Pyrococcus furiosus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.5.1.104 0.06
-
 propane-1,3-diamine pH 7.4, 70°C Pyrococcus furiosus
2.5.1.104 0.1
-
 agmatine pH 7.4, 70°C Pyrococcus furiosus
2.5.1.104 0.18
-
 putrescine pH 7.4, 70°C Pyrococcus furiosus
2.5.1.104 2.09
-
 cadaverine pH 7.4, 70°C Pyrococcus furiosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.104 7.4
-
 assay at Pyrococcus furiosus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5.1.104 0.023
-
 putrescine pH 7.4, 70°C Pyrococcus furiosus
2.5.1.104 0.032
-
 propane-1,3-diamine pH 7.4, 70°C Pyrococcus furiosus
2.5.1.104 0.55
-
 cadaverine pH 7.4, 70°C Pyrococcus furiosus
2.5.1.104 13.4
-
 agmatine pH 7.4, 70°C Pyrococcus furiosus