EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.210 | gene wecD, expression of N-terminal fusion protein with a noncleavable His6-tag in Escherichia coli strain BL21(DE3) | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.3.1.210 | purified recombinant N-terminally His6-tagged WecD free and in complex with acetyl-CoA, hanging drop vapour diffusion method, mixing of 0.001 ml of 7.1 mg/ml protein in a 20 mM Tris-Cl, pH 8.0, 450 mM NaCl, and 5 mM DTT, with 0.001 ml reservoir solution aontaining 100 mM Na-acetate, pH 4.5, 10 mM ZnSO4, 20°C, 1 day, X-ray diffraction structure determination and analysis at 1.66-1.95 A resolution | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.210 | acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose | Escherichia coli | - |
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose | - |
? | |
2.3.1.210 | acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose | Escherichia coli CFT073 | - |
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.210 | Escherichia coli | Q8FBQ3 | gene wecD | - |
2.3.1.210 | Escherichia coli CFT073 | Q8FBQ3 | gene wecD | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.210 | recombinant N-terminally His6-tagged WecD from Escherichia coli strain BL21(DE3) | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.210 | acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose | - |
Escherichia coli | CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose | - |
? | |
2.3.1.210 | acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose | acetyl-CoA binding site structure, overview | Escherichia coli | CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose | - |
? | |
2.3.1.210 | acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose | - |
Escherichia coli CFT073 | CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose | - |
? | |
2.3.1.210 | acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose | acetyl-CoA binding site structure, overview | Escherichia coli CFT073 | CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.210 | dimer | WecD is a dimeric enzyme, with each monomer adopting the GNAT N-acetyltransferase fold, gel filtration and dynamic light scattering, three-dimensional structure comparison, overview | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.210 | TDP-fucosamine acetyltransferase | - |
Escherichia coli |
2.3.1.210 | WecD | - |
Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.210 | acetyl-CoA | - |
Escherichia coli |
EC Number | General Information | Comment | Organism |
---|---|---|---|
2.3.1.210 | evolution | WecD is a GNAT family member. The GNAT N-acetyltransferase fold is common to a number of enzymes involved in acetylation of histones, aminoglycoside antibiotics, serotonin, and sugars | Escherichia coli |
2.3.1.210 | additional information | usage of flexible docking to generate a WecD-bound model of the acetyl-CoATDP-fucosamine tetrahedral intermediate, representing the structure during acetyl transfer. that WecD does not possess a residue that directly functions as a catalytic base, although Tyr208 is well positioned to function as a general acid by protonating the thiolate anion of CoA, modeling of TDP-D-Fuc4N:acetyl-CoA transition state bound to WecD, overview. The WecD tetrahedral intermediate results from nucleophilic attack of the 4-amino group of TDP-fucosamine at the acetyl-CoA thioester carbon atom | Escherichia coli |
2.3.1.210 | physiological function | the enzyme is involved in biosynthesis of enterobacterial common antigen, ECA, a polysaccharide found on the outer membrane of virtually all Gram-negative enteric bacteria, that consists of three sugars, N-acetyl-D-glucosamine, N-acetyl-D-mannosaminuronic acid, and 4-acetamido-4,6-dideoxy-D-galactose, organized into trisaccharide repeating units having the sequence ->3)-alpha-D-Fuc4NAc-(1->4)-beta-D-ManNAcA-(1->4)-alpha-D-GlcNAc-(1-> | Escherichia coli |