Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Scott, J.W.; Rasche, M.E.
    Purification, overproduction, and partial characterization of beta-RFAP synthase, a key enzyme in the methanopterin biosynthesis pathway (2002), J. Bacteriol., 184, 4442-4448.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.54 expression in Escherichia coli Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.54 32600
-
 2 * 32600, SDS-PAGE Methanosarcina thermophila
2.4.2.54 35500
-
 2 * 35500, SDS-PAGE Archaeoglobus fulgidus
2.4.2.54 57000
-
 gel filtration Archaeoglobus fulgidus
2.4.2.54 64500
-
 gel filtration Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.54 4-aminobenzoate + 5-phospho-alpha-D-ribose 1-diphosphate Archaeoglobus fulgidus the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
 ?
2.4.2.54 5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate Methanosarcina thermophila the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.54 Archaeoglobus fulgidus O28190
-
-
2.4.2.54 Methanosarcina thermophila
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.54
-
 Methanosarcina thermophila
2.4.2.54 partial Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.54 0.075
-
 50°C, pH 5.0 Methanosarcina thermophila
2.4.2.54 0.29
-
 50°C, pH 5.0 Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.54 4-aminobenzoate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Archaeoglobus fulgidus 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
 ?
2.4.2.54 4-aminobenzoate + 5-phospho-alpha-D-ribose 1-diphosphate the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria Archaeoglobus fulgidus 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
 ?
2.4.2.54 5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate
-
 Methanosarcina thermophila 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
 ?
2.4.2.54 5-phospho-alpha-D-ribose 1-diphosphate + 4-aminobenzoate the enzyme is involved in biosynthesis of tetrahydromethanopterin, a folate analog involved in the C1 metabolism of methanogenic archaea, sulfate-reducing archaea, and methylotrophic bacteria Methanosarcina thermophila 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate + CO2 + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.54 dimer 2 * 32600, SDS-PAGE Methanosarcina thermophila
2.4.2.54 dimer 2 * 35500, SDS-PAGE Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.54 4-(beta-D-ribofuranosyl)aminobenzene 5'-phosphate synthase
-
 Methanosarcina thermophila
2.4.2.54 AF2089
-
 Archaeoglobus fulgidus
2.4.2.54 beta-RFAP synthase
-
 Methanosarcina thermophila
2.4.2.54 beta-RFAP synthase
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.54 50
-
 assay at Methanosarcina thermophila
2.4.2.54 70
-
-
 Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.54 5
-
 assay at Methanosarcina thermophila
2.4.2.54 5.3
-
-
 Archaeoglobus fulgidus