EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.3.43 | analysis | development of a coupled enzyme assays including 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide and phenazine methosulfate, resulting in the production of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide formazan which can be monitored at 570 nm | Streptomyces fradiae |
1.1.3.44 | analysis | development of a coupled enzyme assays including 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide and phenazine methosulfate, resulting in the production of 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazoliumbromide formazan which can be monitored at 570 nm | Streptomyces lividus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.3.43 | expression in Escherichia coli | Streptomyces fradiae |
1.1.3.44 | expression in Escherichia coli | Streptomyces fradiae |
1.1.3.44 | expression in Escherichia coli | Streptomyces lividus |
2.6.1.93 | expressed in Escherichia coli | Streptomyces fradiae |
2.6.1.95 | expressed in Escherichia coli | Streptomyces fradiae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.3.43 | Streptomyces fradiae | Q53U15 | - |
- |
1.1.3.43 | Streptomyces fradiae NCIMB 8233 | Q53U15 | - |
- |
1.1.3.44 | Streptomyces fradiae | Q53U15 | - |
- |
1.1.3.44 | Streptomyces fradiae NCIMB 8233 | Q53U15 | - |
- |
1.1.3.44 | Streptomyces lividus | Q2MF66 | - |
- |
2.6.1.93 | Streptomyces fradiae | - |
- |
- |
2.6.1.95 | Streptomyces fradiae | - |
- |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.3.43 | 4.2 | - |
pH 7.5, temperature not specified in the publication | Streptomyces fradiae |
1.1.3.44 | 3.5 | - |
pH 7.5, temperature not specified in the publication | Streptomyces lividus |
1.1.3.44 | 6.4 | - |
pH 7.5, temperature not specified in the publication | Streptomyces fradiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.3.43 | paromamine + O2 | - |
Streptomyces fradiae | 6'-dehydro-paromamine + H2O2 | - |
? | |
1.1.3.43 | paromamine + O2 | - |
Streptomyces fradiae NCIMB 8233 | 6'-dehydro-paromamine + H2O2 | - |
? | |
1.1.3.44 | 2'''-N-acetyl-6'''-hydroxyneomycin C + O2 | about 15% of the rate with 6'''-deamino-6'''-hydroxyneomycin C | Streptomyces fradiae | 2'''-N-acetyl-6'''-oxoneomycin C + H2O2 | - |
? | |
1.1.3.44 | 2'''-N-acetyl-6'''-hydroxyneomycin C + O2 | less than 10% of the rate with 6'''-deamino-6'''-hydroxyneomycin C | Streptomyces lividus | 2'''-N-acetyl-6'''-oxoneomycin C + H2O2 | - |
? | |
1.1.3.44 | 2'''-N-acetyl-6'''-hydroxyneomycin C + O2 | about 15% of the rate with 6'''-deamino-6'''-hydroxyneomycin C | Streptomyces fradiae NCIMB 8233 | 2'''-N-acetyl-6'''-oxoneomycin C + H2O2 | - |
? | |
1.1.3.44 | 6'''-deamino-6'''-hydroxyneomycin C + O2 | - |
Streptomyces fradiae | 6'''-deamino-6'''-oxoneomycin C + H2O2 | - |
? | |
1.1.3.44 | 6'''-deamino-6'''-hydroxyneomycin C + O2 | - |
Streptomyces lividus | 6'''-deamino-6'''-oxoneomycin C + H2O2 | - |
? | |
1.1.3.44 | 6'''-deamino-6'''-hydroxyneomycin C + O2 | - |
Streptomyces fradiae NCIMB 8233 | 6'''-deamino-6'''-oxoneomycin C + H2O2 | - |
? | |
1.1.3.44 | additional information | enzyme does not catalyze the reaction of EC 1.1.3.43, paromamine 6'-oxidase | Streptomyces lividus | ? | - |
? | |
2.6.1.93 | 6'-dehydroparomamine + L-glutamate | - |
Streptomyces fradiae | neamine + 2-oxoglutarate | - |
? | |
2.6.1.95 | additional information | NeoB is a bifunctional enzyme that also catalyses the NH2 group transfer at the 6'''-position | Streptomyces fradiae | ? | - |
? | |
2.6.1.95 | neomycin C + 2-oxoglutarate | - |
Streptomyces fradiae | 6'''-hydroxyneomycin C + L-glutamate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.3.43 | neoQ | - |
Streptomyces fradiae |
1.1.3.44 | LivQ | - |
Streptomyces lividus |
1.1.3.44 | neoQ | - |
Streptomyces fradiae |
2.6.1.93 | neoB | - |
Streptomyces fradiae |
2.6.1.95 | neoB | - |
Streptomyces fradiae |