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Literature summary extracted from

  • Kudo, F.; Yamamoto, Y.; Yokoyama, K.; Eguchi, T.; Kakinuma, K.
    Biosynthesis of 2-deoxystreptamine by three crucial enzymes in Streptomyces fradiae NBRC 12773 (2005), J. Antibiot., 58, 766-774.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.329 expression in Escherichia coli Streptomyces fradiae
2.6.1.100 gene neoB, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli Streptomyces fradiae
2.6.1.101 gene neoB, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli Streptomyces fradiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.329 Zn2+ assay in presence of Streptomyces fradiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.100 L-glutamine + 2-deoxy-scyllo-inosose Streptomyces fradiae
-
2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
?
2.6.1.100 L-glutamine + 2-deoxy-scyllo-inosose Streptomyces fradiae NBRC 12773
-
2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
?
2.6.1.101 L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose Streptomyces fradiae
-
2-oxoglutaramate + 2-deoxystreptamine
-
?
2.6.1.101 L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose Streptomyces fradiae NBRC 12773
-
2-oxoglutaramate + 2-deoxystreptamine
-
?
2.6.1.101 additional information Streptomyces fradiae NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 ?
-
?
2.6.1.101 additional information Streptomyces fradiae NBRC 12773 NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.329 Streptomyces fradiae Q53U21
-
-
1.1.1.329 Streptomyces fradiae NBRC 12773 Q53U21
-
-
2.6.1.100 Streptomyces fradiae Q53U20 gene neoB, encoded in the neomycin biosynthetic gene cluster
-
2.6.1.100 Streptomyces fradiae NBRC 12773 Q53U20 gene neoB, encoded in the neomycin biosynthetic gene cluster
-
2.6.1.101 Streptomyces fradiae Q53U20 gene neoB, encoded in the neomycin biosynthetic gene cluster
-
2.6.1.101 Streptomyces fradiae NBRC 12773 Q53U20 gene neoB, encoded in the neomycin biosynthetic gene cluster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.329 2-deoxy-scyllo-inosamine + NAD+
-
Streptomyces fradiae 3-amino-2,3-dideoxy-scyllo-inosose + NADH + H+
-
?
1.1.1.329 2-deoxy-scyllo-inosamine + NAD+
-
Streptomyces fradiae NBRC 12773 3-amino-2,3-dideoxy-scyllo-inosose + NADH + H+
-
?
1.1.1.329 2-deoxy-scyllo-inosamine + NADP+
-
Streptomyces fradiae 3-amino-2,3-dideoxy-scyllo-inosose + NADPH + H+
-
?
1.1.1.329 2-deoxy-scyllo-inosamine + NADP+
-
Streptomyces fradiae NBRC 12773 3-amino-2,3-dideoxy-scyllo-inosose + NADPH + H+
-
?
2.6.1.100 L-glutamine + 2-deoxy-scyllo-inosose
-
Streptomyces fradiae 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
?
2.6.1.100 L-glutamine + 2-deoxy-scyllo-inosose
-
Streptomyces fradiae NBRC 12773 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
?
2.6.1.100 additional information NeoB also shows L-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-streptamine, EC 2.6.1.101 Streptomyces fradiae ?
-
?
2.6.1.100 additional information NeoB also shows L-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-streptamine, EC 2.6.1.101 Streptomyces fradiae NBRC 12773 ?
-
?
2.6.1.101 L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose
-
Streptomyces fradiae 2-oxoglutaramate + 2-deoxystreptamine
-
?
2.6.1.101 L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose
-
Streptomyces fradiae NBRC 12773 2-oxoglutaramate + 2-deoxystreptamine
-
?
2.6.1.101 additional information NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 Streptomyces fradiae ?
-
?
2.6.1.101 additional information NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 Streptomyces fradiae NBRC 12773 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.329 neoA
-
Streptomyces fradiae
2.6.1.100 Neo6
-
Streptomyces fradiae
2.6.1.100 neoB
-
Streptomyces fradiae
2.6.1.101 Gln:DOI aminotransferase
-
Streptomyces fradiae
2.6.1.101 L-glutamine:DOI aminotransferase
-
Streptomyces fradiae
2.6.1.101 Neo6
-
Streptomyces fradiae
2.6.1.101 neoB
-
Streptomyces fradiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.101 28
-
assay at Streptomyces fradiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.101 8
-
assay at Streptomyces fradiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.329 NAD+ enzyme accepts both NAD+ and NADP+ Streptomyces fradiae
1.1.1.329 NADP+ enzyme accepts both NAD+ and NADP+ Streptomyces fradiae
2.6.1.100 pyridoxal 5'-phosphate
-
Streptomyces fradiae
2.6.1.101 pyridoxal 5'-phosphate
-
Streptomyces fradiae

General Information

EC Number General Information Comment Organism
2.6.1.100 metabolism the enzyme is involved in the biosynthesis of 2-deoxystreptamine (DOS)-containing aminoglycoside neomycin. NeoB also shows L-glutamine:3-amino-2,3-dideoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-streptamine, EC 2.6.1.101 Streptomyces fradiae
2.6.1.100 additional information the equivalent of gene neoB, from neo gene cluster, in the btr gene cluster is gene btrS Streptomyces fradiae
2.6.1.101 metabolism the enzyme is involved in the biosynthesis of the 2-deoxystreptamine (DOS)-containing aminoglycoside neomycin. 3-Amino-2,3-dideoxy-scyllo-inosose produced by the NeoA reaction is continuously used as substrate for the natural forward transamination catalyzed by NeoB in the 2-deoxystreptamine biosynthesis. NeoB also shows L-glutamine:2-deoxy-scyllo-inosose aminotransferase activity resulting in formation of 2-deoxy-scyllo-inosamine, EC 2.6.1.100 Streptomyces fradiae
2.6.1.101 additional information the equivalent of gene neoB, from neo gene cluster, in the btr gene cluster is gene btrS Streptomyces fradiae