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Literature summary extracted from

  • Trefzer, C.; Skovierova, H.; Buroni, S.; Bobovska, A.; Nenci, S.; Molteni, E.; Pojer, F.; Pasca, M.; Makarov, V.; Cole, S.; Riccardi, G.; Mikusova, K.; Johnsson, K.
    Benzothiazinones are suicide inhibitors of mycobacterial decaprenylphosphoryl-beta-D-ribofuranose 2'-oxidase DprE1 (2012), J. Am. Chem. Soc., 134, 912-915.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.333 expression in Escherichia coli Mycolicibacterium smegmatis
1.1.98.3 expression in Escherichia coli Mycolicibacterium smegmatis

Protein Variants

EC Number Protein Variants Comment Organism
1.1.98.3 C394G benzothiazinone-resistant mutant, reduces benzothiazinones to inert metabolites while avoiding covalent inactivation Mycolicibacterium smegmatis
1.1.98.3 C394S benzothiazinone-resistant mutant, reduces benzothiazinones to inert metabolites while avoiding covalent inactivation Mycolicibacterium smegmatis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.98.3 2-[(2S)-2-methyl-1,4-dioxa-8-azaspiro[4.5]dec-8-yl]-8-nitro-6-(trifluoromethyl)-4H-1,3-benzothiazin-4-one i.e. BTZ043, inhibits enzyme by formation of a semimercaptal Mycolicibacterium smegmatis

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.333 Mycolicibacterium smegmatis A0R610
-
-
1.1.98.3 Mycolicibacterium smegmatis A0R607
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.333 additional information decaprenylphosphoryl-beta-D-ribofuranose 2'-epimerase is constituted of decaprenylphospho-beta-D-ribofuranose 2-oxidase DprE1 and decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose 2-reductase DprE2 that together catalyze the epimerization of decaprenylphosphoryl-beta-D-ribofuranose to decaprenylphosphoryl-beta-D-arabinofuranose Mycolicibacterium smegmatis ?
-
 ?
1.1.1.333 trans,octacis-decaprenylphospho-beta-D-erythro-pentafuranosid-2-ulose + NADH reaction catalyzed by decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose 2-reductase DprE2 Mycolicibacterium smegmatis trans,octacis-decaprenylphospho-beta-D-arabinofuranose + NAD+
-
 ?
1.1.98.3 (2Z,6E)-farnesylphosphoryl-beta-D-ribofuranose + FAD
-
 Mycolicibacterium smegmatis (2Z,6E)-farnesylphosphoryl-D-beta-D-erythro-pentofuranosid-2-ulose + FADH2 oxidation reaction calyzed by decaprenylphospho-beta-D-ribofuranose 2-oxidase DprE1 ?
1.1.98.3 additional information decaprenylphosphoryl-beta-D-ribofuranose 2'-epimerase is constituted of decaprenylphospho-beta-D-ribofuranose 2-oxidase DprE1 and decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose 2-reductase DprE2 that together catalyze the epimerization of decaprenylphosphoryl-beta-D-ribofuranose to decaprenylphosphoryl-beta-D-arabinofuranose Mycolicibacterium smegmatis ?
-
 ?
1.1.98.3 trans,octacis-decaprenylphospho-beta-D-ribofuranose + FAD
-
 Mycolicibacterium smegmatis trans,octacis-decaprenylphospho-beta-D-erythro-pentofuranosid-2-ulose + FADH2 oxidation reaction catalyzed by decaprenylphospho-beta-D-ribofuranose 2-oxidase DprE1 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.333 DprE2
-
 Mycolicibacterium smegmatis
1.1.1.333 MSMEG_6385
-
 Mycolicibacterium smegmatis
1.1.98.3 DprE1
-
 Mycolicibacterium smegmatis
1.1.98.3 MSMEG_6382
-
 Mycolicibacterium smegmatis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.333 NADH
-
 Mycolicibacterium smegmatis
1.1.98.3 FAD
-
 Mycolicibacterium smegmatis

General Information

EC Number General Information Comment Organism
1.1.1.333 physiological function decaprenylphosphoryl-beta-D-ribofuranose 2'-epimerase is constituted of DprE1 and DprE2 that together catalyze the epimerization of decaprenylphosphoryl-beta-D-ribofuranose to decaprenylphosphoryl-beta-D-arabinofuranose Mycolicibacterium smegmatis
1.1.98.3 physiological function decaprenylphosphoryl-beta-D-ribofuranose 2'-epimerase is constituted of DprE1 and DprE2 that together catalyze the epimerization of decaprenylphosphoryl-beta-D-ribofuranose to decaprenylphosphoryl-beta-D-arabinofuranose Mycolicibacterium smegmatis