Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Chiba, Y.; Terada, T.; Kameya, M.; Shimizu, K.; Arai, H.; Ishii, M.; Igarashi, Y.
    Mechanism for folate-independent aldolase reaction catalyzed by serine hydroxymethyltransferase (2012), FEBS J., 279, 504-514.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 gene HTH1832, overexpression in Escherichia coli Hydrogenobacter thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.1 E53Q site-directed mutagenesis, mutation of a substrate binding residue, the mutant retains tetrahydrofolate-independent aldolase activity Geobacillus stearothermophilus
2.1.2.1 E74Q site-directed mutagenesis, mutation of a substrate binding residue, the mutant retains tetrahydrofolate-independent aldolase activity Ovis aries
2.1.2.1 E75L site-directed mutagenesis, mutation of a substrate binding residue, the mutant retains tetrahydrofolate-independent aldolase activity Oryctolagus cuniculus
2.1.2.1 E75Q site-directed mutagenesis, mutation of a substrate binding residue, the mutant retains tetrahydrofolate-independent aldolase activity Oryctolagus cuniculus
2.1.2.1 H147N site-directed mutagenesis, mutation of a substrate binding residue, the mutant retains tetrahydrofolate-independent aldolase activity Ovis aries
2.1.2.1 Y61A site-directed mutagenesis, the bsSHMT mutant has lost aldolase activity to L-allo-threonine Geobacillus stearothermophilus
2.1.2.1 Y61F site-directed mutagenesis, the bsSHMT mutant has lost aldolase activity to L-allo-threonine Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.2.1 0.28
-
L-serine pH 7.5, 70°C Hydrogenobacter thermophilus
2.1.2.1 0.68
-
glycine pH 7.5, 70°C Hydrogenobacter thermophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytosol
-
Oryctolagus cuniculus 5829
-
2.1.2.1 cytosol
-
Ovis aries 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 48700
-
2 * 48700, SDS-PAGE Hydrogenobacter thermophilus
2.1.2.1 74000
-
gel filtration Hydrogenobacter thermophilus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Oryctolagus cuniculus
-
tetrahydrofolate + L-serine
-
?
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Geobacillus stearothermophilus
-
tetrahydrofolate + L-serine
-
?
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Ovis aries
-
tetrahydrofolate + L-serine
-
?
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Hydrogenobacter thermophilus
-
tetrahydrofolate + L-serine
-
?
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O Hydrogenobacter thermophilus TK-6 / IAM 12695
-
tetrahydrofolate + L-serine
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Geobacillus stearothermophilus
-
-
-
2.1.2.1 Hydrogenobacter thermophilus
-
gene HTH1832
-
2.1.2.1 Hydrogenobacter thermophilus TK-6 / IAM 12695
-
gene HTH1832
-
2.1.2.1 Oryctolagus cuniculus
-
-
-
2.1.2.1 Ovis aries
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.1 cell culture optimum growth temperature of 70-75°C Hydrogenobacter thermophilus
-
2.1.2.1 liver
-
Oryctolagus cuniculus
-
2.1.2.1 liver
-
Ovis aries
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
Oryctolagus cuniculus tetrahydrofolate + L-serine
-
?
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
Geobacillus stearothermophilus tetrahydrofolate + L-serine
-
?
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
Ovis aries tetrahydrofolate + L-serine
-
?
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
Hydrogenobacter thermophilus tetrahydrofolate + L-serine
-
?
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O tetrahydrofolate-dependent SHMT activity, modeling of substrate binding, overview Oryctolagus cuniculus tetrahydrofolate + L-serine
-
?
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O tetrahydrofolate-dependent SHMT activity, modeling of substrate binding, overview Geobacillus stearothermophilus tetrahydrofolate + L-serine
-
?
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O tetrahydrofolate-dependent SHMT activity, modeling of substrate binding, overview Ovis aries tetrahydrofolate + L-serine
-
?
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O tetrahydrofolate-dependent SHMT activity, modeling of substrate binding, overview Hydrogenobacter thermophilus tetrahydrofolate + L-serine
-
?
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O
-
Hydrogenobacter thermophilus TK-6 / IAM 12695 tetrahydrofolate + L-serine
-
?
2.1.2.1 5,10-methylenetetrahydrofolate + glycine + H2O tetrahydrofolate-dependent SHMT activity, modeling of substrate binding, overview Hydrogenobacter thermophilus TK-6 / IAM 12695 tetrahydrofolate + L-serine
-
?
2.1.2.1 additional information the enzyme also exhibits tetrahydrofolate-independent aldolase activity, EC 4.1.2.5, toward beta-hydroxyamino acids, producing glycine and aldehydes Oryctolagus cuniculus ?
-
?
2.1.2.1 additional information the enzyme also exhibits tetrahydrofolate-independent aldolase activity, EC 4.1.2.5, toward beta-hydroxyamino acids, producing glycine and aldehydes Geobacillus stearothermophilus ?
-
?
2.1.2.1 additional information the enzyme also exhibits tetrahydrofolate-independent aldolase activity, EC 4.1.2.5, toward beta-hydroxyamino acids, producing glycine and aldehydes Hydrogenobacter thermophilus ?
-
?
2.1.2.1 additional information the enzyme also exhibits THF-independent aldolase activity, EC 4.1.2.5, toward beta-hydroxyamino acids, producing glycine and aldehydes Ovis aries ?
-
?
2.1.2.1 additional information the enzyme also exhibits tetrahydrofolate-independent aldolase activity, EC 4.1.2.5, toward beta-hydroxyamino acids, producing glycine and aldehydes Hydrogenobacter thermophilus TK-6 / IAM 12695 ?
-
?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 homodimer
-
Geobacillus stearothermophilus
2.1.2.1 homodimer 2 * 48700, SDS-PAGE Hydrogenobacter thermophilus

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 serine hydroxymethyltransferase
-
Oryctolagus cuniculus
2.1.2.1 serine hydroxymethyltransferase
-
Geobacillus stearothermophilus
2.1.2.1 serine hydroxymethyltransferase
-
Ovis aries
2.1.2.1 serine hydroxymethyltransferase
-
Hydrogenobacter thermophilus
2.1.2.1 SHMT
-
Oryctolagus cuniculus
2.1.2.1 SHMT
-
Geobacillus stearothermophilus
2.1.2.1 SHMT
-
Ovis aries
2.1.2.1 SHMT
-
Hydrogenobacter thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.2.1 70
-
assay at Hydrogenobacter thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.2.1 additional information
-
the enzyme shows high thermostability Hydrogenobacter thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.2.1 5
-
glycine pH 7.5, 70°C Hydrogenobacter thermophilus
2.1.2.1 18.7
-
L-serine pH 7.5, 70°C Hydrogenobacter thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.2.1 7.5
-
assay at Hydrogenobacter thermophilus

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate
-
Oryctolagus cuniculus
2.1.2.1 pyridoxal 5'-phosphate
-
Geobacillus stearothermophilus
2.1.2.1 pyridoxal 5'-phosphate
-
Ovis aries
2.1.2.1 pyridoxal 5'-phosphate dependent on Hydrogenobacter thermophilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.2.1 6.4
-
glycine pH 7.5, 70°C Hydrogenobacter thermophilus
2.1.2.1 66.8
-
L-serine pH 7.5, 70°C Hydrogenobacter thermophilus