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Literature summary extracted from

  • Hoffmann, J.; Bona-Lovasz, J.; Beuttler, H.; Altenbuchner, J.
    In vivo and in vitro studies on the carotenoid cleavage oxygenases from Sphingopyxis alaskensis RB2256 and Plesiocystis pacifica SIR-1 revealed their substrate specificities and non-retinal-forming cleavage activities (2012), FEBS J., 279, 3911-3924.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.65 expression in Escherichia coli Plesiocystis pacifica SIR-1
1.13.11.65 expression in Escherichia coli Sphingopyxis alaskensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.65 0.1229
-
apo-8'-carotenal pH 7.4, 25°C Plesiocystis pacifica SIR-1
1.13.11.65 0.1229
-
apo-8'-carotenal pH 7.4, 25°C Sphingopyxis alaskensis

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.65 Plesiocystis pacifica SIR-1
-
-
-
1.13.11.65 Sphingopyxis alaskensis Q1GSG1
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.65 apo-8'-carotenal + O2
-
Plesiocystis pacifica SIR-1 ? cleavage at the 9'/10'- and 11'/12'-double bond ?
1.13.11.65 apo-8'-carotenal + O2
-
Sphingopyxis alaskensis ? cleavage at the 9'/10'- and 11'/12'-double bond ?
1.13.11.65 astaxanthin + O2
-
Plesiocystis pacifica SIR-1 ? single cleavage site at the 13'/14' double bond ?
1.13.11.65 canthaxanthin + O2
-
Plesiocystis pacifica SIR-1 ? two cleavage sites at the 11'/12' or 13'/14' double bond ?
1.13.11.65 decaprenoxanthin + O2
-
Plesiocystis pacifica SIR-1 ?
-
?
1.13.11.65 dihydroxylycopene + O2 i.e. 1,2,1',2'-tetrahydro-psi,psi-carotene-1,1'-diol Plesiocystis pacifica SIR-1 ?
-
?
1.13.11.65 dihydroxylycopene + O2 i.e. 1,2,1',2'-tetrahydro-psi,psi-carotene-1,1'-diol Sphingopyxis alaskensis ?
-
?
1.13.11.65 hydroxylycopene + O2 i.e. 1,2-dihydro-psi,psi-carotene-1-ol Plesiocystis pacifica SIR-1 ?
-
?
1.13.11.65 hydroxylycopene + O2 i.e. 1,2-dihydro-psi,psi-carotene-1-ol Sphingopyxis alaskensis ?
-
?
1.13.11.65 lycopene + O2
-
Sphingopyxis alaskensis ?
-
?
1.13.11.65 additional information enzyme carotenoid cleavage requires the presence of either hydroxy or keto groups Plesiocystis pacifica SIR-1 ?
-
?
1.13.11.65 additional information enzyme cleaves acyclic and monocyclic substrates Sphingopyxis alaskensis ?
-
?
1.13.11.65 nostoxanthin + O2
-
Plesiocystis pacifica SIR-1 ? two cleavage sites at the 11'/12' or 13'/14' double bond ?
1.13.11.65 sarcinaxanthin + O2
-
Plesiocystis pacifica SIR-1 ?
-
?
1.13.11.65 sarprenoxanthin + O2
-
Plesiocystis pacifica SIR-1 ?
-
?
1.13.11.65 zeaxanthin + O2
-
Plesiocystis pacifica SIR-1 ? two cleavage sites at the 11'/12' or 13'/14' double bond ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.65 ppsir_15490
-
Plesiocystis pacifica SIR-1
1.13.11.65 sala_1698
-
Sphingopyxis alaskensis
1.13.11.65 SIR-1
-
Plesiocystis pacifica SIR-1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.65 4.9
-
apo-8'-carotenal pH 7.4, 25°C Plesiocystis pacifica SIR-1
1.13.11.65 4.9
-
apo-8'-carotenal pH 7.4, 25°C Sphingopyxis alaskensis