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Literature summary extracted from

  • Irimia, A.; Madern, D.; Zaccai, G.; Vellieux, F.M.
    Methanoarchaeal sulfolactate dehydrogenase: prototype of a new family of NADH-dependent enzymes (2004), EMBO J., 23, 1234-1244.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.337 in complex with NADH, to 2.5 A resolution. The asymmetric unit contains a tetramer of tight dimers. Structure does not contain a cofactor-binding domain with Rossmann-fold topology. The NADH is bound in an extended conformation in each active site, in a manner that explains the pro-S specificity. Cofactor binding involves residues belonging to both subunits within the tight dimers. The results indicate the existence of a substrate discrimination mechanism, which involves electrostatic interactions Methanocaldococcus jannaschii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.337 0.04
-
 3-sulfopyruvate pH 8.0, 70°C Methanocaldococcus jannaschii
1.1.1.337 0.13
-
 oxaloacetate pH 8.0, 70°C Methanocaldococcus jannaschii
1.1.1.337 1.9
-
 2-oxopentanedioic acid pH 8.0, 70°C Methanocaldococcus jannaschii
1.1.1.337 3.4
-
 oxopropandioic acid pH 8.0, 70°C Methanocaldococcus jannaschii
1.1.1.337 46
-
 glyoxylate pH 8.0, 70°C Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.337 75000
-
 2 * 75000, calculated Methanocaldococcus jannaschii
1.1.1.337 150000
-
 gel filtration and velocity sedimentation data Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.337 Methanocaldococcus jannaschii Q58820
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.337 2-oxopentanedioic acid + NADH + H+
-
 Methanocaldococcus jannaschii 2-hydroxypentandioic acid + NAD+
-
 ?
1.1.1.337 3-sulfopyruvate + NADH + H+
-
 Methanocaldococcus jannaschii 3-sulfolactate + NAD+
-
 ?
1.1.1.337 glyoxylate + NADH + H+
-
 Methanocaldococcus jannaschii 2-hydroxypropanoate + NAD+
-
 ?
1.1.1.337 additional information no substrates: pyruvate, 2-oxobutanoate, 2-oxovalerate, ketoisovalerate, 2-oxohexanedioate, 2-ketohexanedioate, 3-methyl 2-oxovalerate and 4-methyl-2-oxovalerate Methanocaldococcus jannaschii ?
-
 ?
1.1.1.337 oxaloacetate + NADH + H+
-
 Methanocaldococcus jannaschii malate + NAD+
-
 ?
1.1.1.337 oxopropandioic acid + NADH + H+
-
 Methanocaldococcus jannaschii 2-hydroxypropanedioic acid + NAD+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.337 dimer 2 * 75000, calculated Methanocaldococcus jannaschii